Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system

Zhivin, Olga; Dassa, Bareket; Moraïs, Sarah; Utturkar, Sagar M.; Brown, Steven D.; Henrissat, Bernard; Lamed, Raphael; Bayer, Edward A.

doi:10.1186/s13068-017-0898-6

Title: Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system

Journal Article · Thu Sep 07 00:00:00 EDT 2017 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-017-0898-6· OSTI ID:1394357

Zhivin, Olga ^[1]; Dassa, Bareket ^[1]; Moraïs, Sarah ^[1]; Utturkar, Sagar M. ^[2]; Brown, Steven D. ^[3]; Henrissat, Bernard ^[4]; Lamed, Raphael ^[5];

^[1]

Weizmann Inst. of Science, Rehovot (Israel). Dept. of Biomolecular Sciences
Univ. of Tennessee, Knoxville, TN (United States). Graduate School of Genome Science and Technology; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
Univ. of Tennessee, Knoxville, TN (United States). Graduate School of Genome Science and Technology; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division, Energy and Environment Directorate
Aix-Marseille Univ., and CNRS/IN2P3, Marseille (France). Architecture et Fonction des Macromolecules Biologiques
Tel Aviv Univ., Ramat Aviv (Israel). Dept. of Molecular Microbiology and Biotechnology

The organization of the B. cellulosolvens cellulosome is unique compared to previously described cellulosome systems. In contrast to all other known cellulosomes, the cohesin types are reversed for all scaffoldins i.e., the type II cohesins are located on the enzyme-integrating primary scaffoldin, whereas the type I cohesins are located on the anchoring scaffoldins. Many of the type II dockerin-bearing ORFs include X60 modules, which are known to stabilize type II cohesin–dockerin interactions. In the present work, we focused on revealing the architectural arrangement of cellulosome structure in this bacterium by examining numerous interactions between the various cohesin and dockerin modules. In total, we cloned and expressed 43 representative cohesins and 27 dockerins. The results revealed various possible architectures of cell-anchored and cell-free cellulosomes, which serve to assemble distinctive cellulosome types via three distinct cohesin–dockerin specificities: type I, type II, and a novel-type designated R (distinct from type III interactions, predominant in ruminococcal cellulosomes). The results of this study provide novel insight into the architecture and function of the most intricate and extensive cellulosomal system known today, thereby extending significantly our overall knowledge base of cellulosome systems and their components. The robust cellulosome system of B. cellulosolvens, with its unique binding specificities and reversal of cohesin–dockerin types, has served to amend our view of the cellulosome paradigm. Revealing new cellulosomal interactions and arrangements is critical for designing high-efficiency artificial cellulosomes for conversion of plant-derived cellulosic biomass towards improved production of biofuels.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); Israel Science Foundation (ISF); United States-Israel Binational Science Foundation (BSF); National Natural Science Foundation of China (NSFC)

Grant/Contract Number:: AC05-00OR22725; 2566/16; 1349

OSTI ID:: 1394357

Journal Information:: Biotechnology for Biofuels, Vol. 10, Issue 1; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 21 works

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Inducing effects of cellulosic hydrolysate components of lignocellulose on cellulosome synthesis in Clostridium thermocellum Li, Renmin; Feng, Yingang; Liu, Shiyue Microbial Biotechnology, Vol. 11, Issue 5 https://doi.org/10.1111/1751-7915.13293	journal	June 2018
Creation of a functional hyperthermostable designer cellulosome Kahn, Amaranta; Moraïs, Sarah; Galanopoulou, Anastasia P. Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1386-y	journal	February 2019
Unraveling essential cellulosomal components of the (Pseudo)Bacteroides cellulosolvens reveals an extensive reservoir of novel catalytic enzymes Zhivin-Nissan, Olga; Dassa, Bareket; Morag, Ely Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1447-2	journal	May 2019
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Glgycoside hydrolases

Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system Zhivin, Olga; Dassa, Bareket; Morais, Sarah https://doi.org/10.6084/M9.FIGSHARE.C.3874348 The current record is supplemented by this dataset	dataset	September 2017
Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system [Supplementary Data] Zhivin, Olga; Dassa, Bareket; Moraïs, Sarah https://doi.org/10.6084/m9.figshare.c.3874348.v1 The current record is supplemented by this collection	collection	September 2017
MOESM1 of Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system Zhivin, Olga; Dassa, Bareket; Moraïs, Sarah https://doi.org/10.6084/m9.figshare.c.3874348_d1 The current record is supplemented by this dataset	dataset	September 2017
MOESM2 of Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system Zhivin, Olga; Dassa, Bareket; MoraĂŻ, Sarah https://doi.org/10.6084/m9.figshare.c.3874348_d2.v1 The current record is supplemented by this dataset	dataset	September 2017
MOESM3 of Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system Zhivin, Olga; Dassa, Bareket; Moraïs, Sarah https://doi.org/10.6084/m9.figshare.c.3874348_d3.v1 The current record is supplemented by this dataset	dataset	September 2017
MOESM4 of Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system Zhivin, Olga; Moraïs, Sarah; Utturkar, Sagar https://doi.org/10.6084/m9.figshare.c.3874348_d4 The current record is supplemented by this figure	figure	September 2017
MOESM5 of Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system Zhivin, Olga; Dassa, Bareket; Moraïs, Sarah https://doi.org/10.6084/m9.figshare.c.3874348_d5.v1 The current record is supplemented by this figure	figure	September 2017
MOESM6 of Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system Zhivin, Olga; Dassa, Bareket; Moraïs, Sarah https://doi.org/10.6084/m9.figshare.c.3874348_d6 The current record is supplemented by this dataset	dataset	September 2017
MOESM7 of Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system [Supplemental Data] Zhivin, Olga; Dassa, Bareket; Moraïs, Sarah https://doi.org/10.6084/m9.figshare.c.3874348_d7 The current record is supplemented by this dataset	dataset	September 2017
MOESM8 of Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system Zhivin, Olga; Dassa, Bareket; MoraAZs, Sarah https://doi.org/10.6084/m9.figshare.c.3874348_d8.v1 The current record is supplemented by this dataset	dataset	September 2017
MOESM3 of Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system Zhivin, Olga; Dassa, Bareket; Moraïs, Sarah https://doi.org/10.6084/m9.figshare.c.3874348_d3 The current record is supplemented by this dataset	dataset	September 2017
MOESM6 of Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system Zhivin, Olga; Dassa, Bareket; MoraAZs, Sarah https://doi.org/10.6084/m9.figshare.c.3874348_d6.v1 The current record is supplemented by this dataset	dataset	September 2017
MOESM7 of Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system Zhivin, Olga; Dassa, Bareket; MoraAZs, Sarah https://doi.org/10.6084/m9.figshare.c.3874348_d7.v1 The current record is supplemented by this dataset	dataset	September 2017
MOESM2 of Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system Zhivin, Olga; Moraïs, Sarah; Utturkar, Sagar https://doi.org/10.6084/m9.figshare.c.3874348_d2 The current record is supplemented by this dataset	dataset	September 2017
MOESM4 of Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system Zhivin, Olga; Dassa, Bareket; MoraĂŻs, Sarah https://doi.org/10.6084/m9.figshare.c.3874348_d4.v1 The current record is supplemented by this dataset	dataset	September 2017
MOESM5 of Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system Zhivin, Olga; Dassa, Bareket; Moraïs, Sarah https://doi.org/10.6084/m9.figshare.c.3874348_d5 The current record is supplemented by this dataset	dataset	September 2017
MOESM8 of Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system Zhivin, Olga; Dassa, Bareket; Moraïs, Sarah https://doi.org/10.6084/m9.figshare.c.3874348_d8 The current record is supplemented by this dataset	dataset	September 2017
MOESM1 of Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system Zhivin, Olga; Dassa, Bareket; Utturkar, Sagar https://doi.org/10.6084/m9.figshare.c.3874348_d1.v1 The current record is supplemented by this dataset	dataset	September 2017