Understanding Cultivar-Specificity and Soil Determinants of the Cannabis Microbiome

Winston, Max E.; Hampton-Marcell, Jarrad; Zarraonaindia, Iratxe; Owens, Sarah M.; Moreau, Corrie S.; Gilbert, Jack A.; Hartsel, Josh; Kennedy, Suzanne J.; Gibbons, Sean M.; Berg, Gabriele

doi:10.1371/journal.pone.0099641

Title: Understanding Cultivar-Specificity and Soil Determinants of the Cannabis Microbiome

Journal Article · Mon Jun 16 00:00:00 EDT 2014 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0099641· OSTI ID:1392596

Winston, Max E. ^[1]; Hampton-Marcell, Jarrad ^[2]; Zarraonaindia, Iratxe ^[3]; Owens, Sarah M. ^[2]; Moreau, Corrie S. ^[4]; Gilbert, Jack A. ^[2]; Hartsel, Josh ^[5]; Kennedy, Suzanne J. ^[6]; Gibbons, Sean M. ^[2]; Berg, Gabriele ^[7]

Dept. of Science and Education, Chicago, IL (United States); Univ. of Chicago, Chicago, IL (United States)
Argonne National Lab. (ANL), Lemont, IL (United States); Univ. of Chicago, Chicago, IL (United States)
Argonne National Lab. (ANL), Lemont, IL (United States); Basque Country Government, Bilbao (Spain)
Dept. of Science and Education, Chicago, IL (United States)
Cannavest, San Diego, CA (United States)
MO BIO Labs, Carlsbad, CA (United States)
Graz Univ. of Technology (TU Graz) (Austria)

Understanding microbial partnerships with the medicinally and economically important crop Cannabis has the potential to affect agricultural practice by improving plant fitness and production yield. Furthermore, Cannabis presents an interesting model to explore plant-microbiome interactions as it produces numerous secondary metabolic compounds. Here we present the first description of the endorhiza-, rhizosphere-, and bulk soil-associated microbiome of five distinct Cannabis cultivars. Bacterial communities of the endorhiza showed significant cultivar-specificity. When controlling cultivar and soil type the microbial community structure was significantly different between plant cultivars, soil types, and between the endorhiza, rhizosphere and soil. In conclusion, the influence of soil type, plant cultivar and sample type differentiation on the microbial community structure provides support for a previously published two-tier selection model, whereby community composition across sample types is determined mainly by soil type, while community structure within endorhiza samples is determined mainly by host cultivar.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: Argonne National Laboratory; USDOE; U.S. Department of Education; USEPA

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1392596

Journal Information:: PLoS ONE, Vol. 9, Issue 6; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 37 works

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Title: Understanding Cultivar-Specificity and Soil Determinants of the Cannabis Microbiome

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