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Title: Sulfide Generation by Dominant Halanaerobium Microorganisms in Hydraulically Fractured Shales

Abstract

ABSTRACT Hydraulic fracturing of black shale formations has greatly increased United States oil and natural gas recovery. However, the accumulation of biomass in subsurface reservoirs and pipelines is detrimental because of possible well souring, microbially induced corrosion, and pore clogging. Temporal sampling of produced fluids from a well in the Utica Shale revealed the dominance ofHalanaerobiumstrains within thein situmicrobial community and the potential for these microorganisms to catalyze thiosulfate-dependent sulfidogenesis. From these field data, we investigated biogenic sulfide production catalyzed by aHalanaerobiumstrain isolated from the produced fluids using proteogenomics and laboratory growth experiments. Analysis ofHalanaerobiumisolate genomes and reconstructed genomes from metagenomic data sets revealed the conserved presence of rhodanese-like proteins and anaerobic sulfite reductase complexes capable of converting thiosulfate to sulfide. Shotgun proteomics measurements using aHalanaerobiumisolate verified that these proteins were more abundant when thiosulfate was present in the growth medium, and culture-based assays identified thiosulfate-dependent sulfide production by the same isolate. Increased production of sulfide and organic acids during the stationary growth phase suggests that fermentativeHalanaerobiumuses thiosulfate to remove excess reductant. These findings emphasize the potential detrimental effects that could arise from thiosulfate-reducing microorganisms in hydraulically fractured shales, which are undetected by current industry-wide corrosion diagnostics. IMPORTANCEAlthough thousands ofmore » wells in deep shale formations across the United States have been hydraulically fractured for oil and gas recovery, the impact of microbial metabolism within these environments is poorly understood. Our research demonstrates that dominant microbial populations in these subsurface ecosystems contain the conserved capacity for the reduction of thiosulfate to sulfide and that this process is likely occurring in the environment. Sulfide generation (also known as “souring”) is considered deleterious in the oil and gas industry because of both toxicity issues and impacts on corrosion of the subsurface infrastructure. Critically, the capacity for sulfide generation via reduction of sulfate was not detected in our data sets. Given that current industry wellhead tests for sulfidogenesis target canonical sulfate-reducing microorganisms, these data suggest that new approaches to the detection of sulfide-producing microorganisms may be necessary.« less

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ; ; ORCiD logo;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1390411
Report Number(s):
PNNL-SA-128950
Journal ID: ISSN 2379-5042; 48483; KP1704020
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: mSphere; Journal Volume: 2; Journal Issue: 4
Country of Publication:
United States
Language:
English
Subject:
04 OIL SHALES AND TAR SANDS; Halanaerobium; shale; thiosulfate; Environmental Molecular Sciences Laboratory

Citation Formats

Booker, Anne E., Borton, Mikayla A., Daly, Rebecca A., Welch, Susan A., Nicora, Carrie D., Hoyt, David W., Wilson, Travis, Purvine, Samuel O., Wolfe, Richard A., Sharma, Shikha, Mouser, Paula J., Cole, David R., Lipton, Mary S., Wrighton, Kelly C., Wilkins, Michael J., and McMahon, Katherine. Sulfide Generation by Dominant Halanaerobium Microorganisms in Hydraulically Fractured Shales. United States: N. p., 2017. Web. doi:10.1128/mSphereDirect.00257-17.
Booker, Anne E., Borton, Mikayla A., Daly, Rebecca A., Welch, Susan A., Nicora, Carrie D., Hoyt, David W., Wilson, Travis, Purvine, Samuel O., Wolfe, Richard A., Sharma, Shikha, Mouser, Paula J., Cole, David R., Lipton, Mary S., Wrighton, Kelly C., Wilkins, Michael J., & McMahon, Katherine. Sulfide Generation by Dominant Halanaerobium Microorganisms in Hydraulically Fractured Shales. United States. doi:10.1128/mSphereDirect.00257-17.
Booker, Anne E., Borton, Mikayla A., Daly, Rebecca A., Welch, Susan A., Nicora, Carrie D., Hoyt, David W., Wilson, Travis, Purvine, Samuel O., Wolfe, Richard A., Sharma, Shikha, Mouser, Paula J., Cole, David R., Lipton, Mary S., Wrighton, Kelly C., Wilkins, Michael J., and McMahon, Katherine. Wed . "Sulfide Generation by Dominant Halanaerobium Microorganisms in Hydraulically Fractured Shales". United States. doi:10.1128/mSphereDirect.00257-17.
@article{osti_1390411,
title = {Sulfide Generation by Dominant Halanaerobium Microorganisms in Hydraulically Fractured Shales},
author = {Booker, Anne E. and Borton, Mikayla A. and Daly, Rebecca A. and Welch, Susan A. and Nicora, Carrie D. and Hoyt, David W. and Wilson, Travis and Purvine, Samuel O. and Wolfe, Richard A. and Sharma, Shikha and Mouser, Paula J. and Cole, David R. and Lipton, Mary S. and Wrighton, Kelly C. and Wilkins, Michael J. and McMahon, Katherine},
abstractNote = {ABSTRACT Hydraulic fracturing of black shale formations has greatly increased United States oil and natural gas recovery. However, the accumulation of biomass in subsurface reservoirs and pipelines is detrimental because of possible well souring, microbially induced corrosion, and pore clogging. Temporal sampling of produced fluids from a well in the Utica Shale revealed the dominance ofHalanaerobiumstrains within thein situmicrobial community and the potential for these microorganisms to catalyze thiosulfate-dependent sulfidogenesis. From these field data, we investigated biogenic sulfide production catalyzed by aHalanaerobiumstrain isolated from the produced fluids using proteogenomics and laboratory growth experiments. Analysis ofHalanaerobiumisolate genomes and reconstructed genomes from metagenomic data sets revealed the conserved presence of rhodanese-like proteins and anaerobic sulfite reductase complexes capable of converting thiosulfate to sulfide. Shotgun proteomics measurements using aHalanaerobiumisolate verified that these proteins were more abundant when thiosulfate was present in the growth medium, and culture-based assays identified thiosulfate-dependent sulfide production by the same isolate. Increased production of sulfide and organic acids during the stationary growth phase suggests that fermentativeHalanaerobiumuses thiosulfate to remove excess reductant. These findings emphasize the potential detrimental effects that could arise from thiosulfate-reducing microorganisms in hydraulically fractured shales, which are undetected by current industry-wide corrosion diagnostics. IMPORTANCEAlthough thousands of wells in deep shale formations across the United States have been hydraulically fractured for oil and gas recovery, the impact of microbial metabolism within these environments is poorly understood. Our research demonstrates that dominant microbial populations in these subsurface ecosystems contain the conserved capacity for the reduction of thiosulfate to sulfide and that this process is likely occurring in the environment. Sulfide generation (also known as “souring”) is considered deleterious in the oil and gas industry because of both toxicity issues and impacts on corrosion of the subsurface infrastructure. Critically, the capacity for sulfide generation via reduction of sulfate was not detected in our data sets. Given that current industry wellhead tests for sulfidogenesis target canonical sulfate-reducing microorganisms, these data suggest that new approaches to the detection of sulfide-producing microorganisms may be necessary.},
doi = {10.1128/mSphereDirect.00257-17},
journal = {mSphere},
number = 4,
volume = 2,
place = {United States},
year = {Wed Jul 05 00:00:00 EDT 2017},
month = {Wed Jul 05 00:00:00 EDT 2017}
}