Single-Molecule Fluorescence Spectroscopy of Photosynthetic Systems

Kondo, Toru; Chen, Wei Jia; Schlau-Cohen, Gabriela S.

doi:10.1021/acs.chemrev.6b00195

Single-Molecule Fluorescence Spectroscopy of Photosynthetic Systems

Journal Article · Tue Jan 10 04:00:00 EST 2017 · Chemical Reviews

DOI:https://doi.org/10.1021/acs.chemrev.6b00195· OSTI ID:1388262

Kondo, Toru ^[1]; ^[1]; ^[1]

Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

Photosynthesis begins when a network of pigment–protein complexes captures solar energy and transports it to the reaction center, where charge separation occurs. When necessary (under low light conditions), photosynthetic organisms perform this energy transport and charge separation with near unity quantum efficiency. Remarkably, this high efficiency is maintained under physiological conditions, which include thermal fluctuations of the pigment–protein complexes and changing local environments. These conditions introduce multiple types of heterogeneity in the pigment–protein complexes, including structural heterogeneity, energetic heterogeneity, and functional heterogeneity. Understanding how photosynthetic light-harvesting functions in the face of these fluctuations requires understanding this heterogeneity, which, in turn, requires characterization of individual pigment–protein complexes. Single-molecule spectroscopy has the power to probe individual complexes. In this review, we present an overview of the common techniques for single-molecule fluorescence spectroscopy applied to photosynthetic systems and describe selected experiments on these systems. We discuss how these experiments provide a new understanding of the impact of heterogeneity on light harvesting and thus how these systems are optimized to capture sunlight under physiological conditions.

View Accepted Manuscript (DOE)

Research Organization:: Energy Frontier Research Centers (EFRC) (United States). Center for Excitonics (CE)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: SC0001088

OSTI ID:: 1388262

Journal Information:: Chemical Reviews, Journal Name: Chemical Reviews Journal Issue: 2 Vol. 117; ISSN 0009-2665

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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Microsecond and millisecond dynamics in the photosynthetic protein LHCSR1 observed by single-molecule correlation spectroscopy Kondo, Toru; Gordon, Jesse B.; Pinnola, Alberta Proceedings of the National Academy of Sciences, Vol. 116, Issue 23 https://doi.org/10.1073/pnas.1821207116	journal	May 2019
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