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Title: Structure of the human TRiC/CCT Subunit 5 associated with hereditary sensory neuropathy

Abstract

The human chaperonin TRiC consists of eight non-identical subunits, and its protein-folding activity is critical for cellular health. Misfolded proteins are associated with many human diseases, such as amyloid diseases, cancer, and neuropathies, making TRiC a potential therapeutic target. A detailed structural understanding of its ATP-dependent folding mechanism and substrate recognition is therefore of great importance. Of particular health-related interest is the mutation Histidine 147 to Arginine (H147R) in human TRiC subunit 5 (CCT5), which has been associated with hereditary sensory neuropathy. In this paper, we describe the crystal structures of CCT5 and the CCT5-H147R mutant, which provide important structural information for this vital protein-folding machine in humans. This first X-ray crystallographic study of a single human CCT subunit in the context of a hexadecameric complex can be expanded in the future to the other 7 subunits that form the TRiC complex.

Authors:
 [1];  [1];  [2];  [3];  [2];  [4]
  1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division
  2. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Biology Dept.
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division, Berkeley Center for Structural Biology
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division; Univ. of California, Berkeley, CA (United States). Dept. of Bioengineering
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1379932
Grant/Contract Number:
AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; chaperones; mechanisms of disease; protein aggregation; x-ray crystallography

Citation Formats

Pereira, Jose H., McAndrew, Ryan P., Sergeeva, Oksana A., Ralston, Corie Y., King, Jonathan A., and Adams, Paul D.. Structure of the human TRiC/CCT Subunit 5 associated with hereditary sensory neuropathy. United States: N. p., 2017. Web. doi:10.1038/s41598-017-03825-3.
Pereira, Jose H., McAndrew, Ryan P., Sergeeva, Oksana A., Ralston, Corie Y., King, Jonathan A., & Adams, Paul D.. Structure of the human TRiC/CCT Subunit 5 associated with hereditary sensory neuropathy. United States. doi:10.1038/s41598-017-03825-3.
Pereira, Jose H., McAndrew, Ryan P., Sergeeva, Oksana A., Ralston, Corie Y., King, Jonathan A., and Adams, Paul D.. Fri . "Structure of the human TRiC/CCT Subunit 5 associated with hereditary sensory neuropathy". United States. doi:10.1038/s41598-017-03825-3. https://www.osti.gov/servlets/purl/1379932.
@article{osti_1379932,
title = {Structure of the human TRiC/CCT Subunit 5 associated with hereditary sensory neuropathy},
author = {Pereira, Jose H. and McAndrew, Ryan P. and Sergeeva, Oksana A. and Ralston, Corie Y. and King, Jonathan A. and Adams, Paul D.},
abstractNote = {The human chaperonin TRiC consists of eight non-identical subunits, and its protein-folding activity is critical for cellular health. Misfolded proteins are associated with many human diseases, such as amyloid diseases, cancer, and neuropathies, making TRiC a potential therapeutic target. A detailed structural understanding of its ATP-dependent folding mechanism and substrate recognition is therefore of great importance. Of particular health-related interest is the mutation Histidine 147 to Arginine (H147R) in human TRiC subunit 5 (CCT5), which has been associated with hereditary sensory neuropathy. In this paper, we describe the crystal structures of CCT5 and the CCT5-H147R mutant, which provide important structural information for this vital protein-folding machine in humans. This first X-ray crystallographic study of a single human CCT subunit in the context of a hexadecameric complex can be expanded in the future to the other 7 subunits that form the TRiC complex.},
doi = {10.1038/s41598-017-03825-3},
journal = {Scientific Reports},
number = 1,
volume = 7,
place = {United States},
year = {Fri Jun 16 00:00:00 EDT 2017},
month = {Fri Jun 16 00:00:00 EDT 2017}
}

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