Production of jet fuel precursor monoterpenoids from engineered Escherichia coli: Production of Jet Fuel Precursor Monoterpenoids

Mendez-Perez, Daniel; Alonso-Gutierrez, Jorge; Hu, Qijun; Molinas, Margaux; Baidoo, Edward E. K.; Wang, George; Chan, Leanne J. G.; Adams, Paul D.; Petzold, Christopher J.; Keasling, Jay D.; Lee, Taek S.

doi:10.1002/bit.26296

Title: Production of jet fuel precursor monoterpenoids from engineered Escherichia coli: Production of Jet Fuel Precursor Monoterpenoids

Journal Article · Thu May 18 00:00:00 EDT 2017 · Biotechnology and Bioengineering

DOI:https://doi.org/10.1002/bit.26296· OSTI ID:1379914

Mendez-Perez, Daniel ^[1]; Alonso-Gutierrez, Jorge ^[1]; Hu, Qijun ^[1]; Molinas, Margaux ^[1]; Baidoo, Edward E. K. ^[1]; Wang, George ^[1]; Chan, Leanne J. G. ^[1]; Adams, Paul D. ^[1]; Petzold, Christopher J. ^[1]; Keasling, Jay D. ^[2];

^[1]

Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Technical Univ. of Denmark, Horsholm (Denmark); Univ. of California, Berkeley, CA (United States)

ABSTRACT Monoterpenes (C ₁₀ isoprenoids) are the main components of essential oils and are possible precursors for many commodity chemicals and high energy density fuels. Monoterpenes are synthesized from geranyl diphosphate (GPP), which is also the precursor for the biosynthesis of farnesyl diphosphate (FPP). FPP biosynthesis diverts the carbon flux from monoterpene production to C ₁₅ products and quinone biosynthesis. In this study, we tested a chromosomal mutation of Escherichia coli 's native FPP synthase (IspA) to improve GPP availability for the production of monoterpenes using a heterologous mevalonate pathway. Monoterpene production at high levels required not only optimization of GPP production but also a basal level of FPP to maintain growth. The optimized strains produced two jet fuel precursor monoterpenoids 1,8‐cineole and linalool at the titer of 653 mg/L and 505 mg/L, respectively, in batch cultures with 1% glucose. The engineered strains developed in this work provide useful resources for the production of high‐value monoterpenes. Biotechnol. Bioeng. 2017;114: 1703–1712. © 2017 Wiley Periodicals, Inc.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1379914

Alternate ID(s):: OSTI ID: 1401291

Journal Information:: Biotechnology and Bioengineering, Vol. 114, Issue 8; ISSN 0006-3592

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 55 works

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