Development of a Transcription Factor-Based Lactam Biosensor

Zhang, Jingwei; Barajas, Jesus F.; Burdu, Mehmet; Ruegg, Thomas L.; Dias, Bryton; Keasling, Jay D.

doi:10.1021/acssynbio.6b00136

Title: Development of a Transcription Factor-Based Lactam Biosensor

Journal Article · Tue Dec 20 00:00:00 EST 2016 · ACS Synthetic Biology

DOI:https://doi.org/10.1021/acssynbio.6b00136· OSTI ID:1379780

Zhang, Jingwei ^[1]; Barajas, Jesus F. ^[2]; Burdu, Mehmet ^[2]; Ruegg, Thomas L. ^[2]; Dias, Bryton ^[2];

^[3]

Univ. of California, Berkeley, CA (United States); Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
Univ. of California, Berkeley, CA (United States); Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Technical Univ. of Denmark, Lyngby (Denmark)

Lactams are an important class of commodity chemicals used in the manufacture of nylons, with millions of tons produced every year. Biological production of lactams could be greatly improved by high-throughput sensors for lactam biosynthesis. To identify biosensors of lactams, we applied in this paper a chemoinformatic approach inspired by small molecule drug discovery. We define this approach as analogue generation toward catabolizable chemicals or AGTC. We discovered a lactam biosensor based on the ChnR/Pb transcription factor-promoter pair. The microbial biosensor is capable of sensing ε-caprolactam, δ-valerolactam, and butyrolactam in a dose-dependent manner. Finally, the biosensor has sufficient specificity to discriminate against lactam biosynthetic intermediates and therefore could potentially be applied for high-throughput metabolic engineering for industrially important high titer lactam biosynthesis.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF)

DOE Contract Number:: AC02-05CH11231; EEC 0540879

OSTI ID:: 1379780

Journal Information:: ACS Synthetic Biology, Vol. 6, Issue 3; ISSN 2161-5063

Publisher:: American Chemical Society (ACS)

Country of Publication:: United States

Language:: English

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