Methane-Oxidizing Bacteria Shunt Carbon to Microbial Mats at a Marine Hydrocarbon Seep

Paul, Blair G.; Ding, Haibing; Bagby, Sarah C.; Kellermann, Matthias Y.; Redmond, Molly C.; Andersen, Gary L.; Valentine, David L.

doi:10.3389/fmicb.2017.00186

Methane-Oxidizing Bacteria Shunt Carbon to Microbial Mats at a Marine Hydrocarbon Seep

Journal Article · Mon Feb 27 00:00:00 EST 2017 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2017.00186· OSTI ID:1379742

Paul, Blair G. ^[1]; Ding, Haibing ^[2]; Bagby, Sarah C. ^[3]; Kellermann, Matthias Y. ^[4]; Redmond, Molly C. ^[5]; Andersen, Gary L. ^[6]; Valentine, David L. ^[1]

Univ. of California, Santa Barbara, CA (United States)
Ocean Univ. of China, Qingdao (China)
Univ. of California, Santa Barbara, CA (United States); Case Western Reserve Univ., Cleveland, OH (United States)
Univ. of California, Santa Barbara, CA (United States); Carl von Ossietzky Univ., Wilhelmshaven (Germany)
Univ. of California, Santa Barbara, CA (United States); Univ. of North Carolina, Charlotte, NC (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

The marine subsurface is a reservoir of the greenhouse gas methane. While microorganisms living in water column and seafloor ecosystems are known to be a major sink limiting net methane transport from the marine subsurface to the atmosphere, few studies have assessed the flow of methane-derived carbon through the benthic mat communities that line the seafloor on the continental shelf where methane is emitted. We analyzed the abundance and isotope composition of fatty acids in microbial mats grown in the shallow Coal Oil Point seep field off Santa Barbara, CA, USA, where seep gas is a mixture of methane and CO₂. We further used stable isotope probing (SIP) to track methane incorporation into mat biomass. We found evidence that multiple allochthonous substrates supported the rich growth of these mats, with notable contributions from bacterial methanotrophs and sulfur-oxidizers as well as eukaryotic phototrophs. Fatty acids characteristic of methanotrophs were shown to be abundant and ¹³C-enriched in SIP samples, and DNA-SIP identified members of the methanotrophic family Methylococcaceae as major ¹³CH₄ consumers. Members of Sulfuricurvaceae, Sulfurospirillaceae, and Sulfurovumaceae are implicated in fixation of seep CO₂. The mats’ autotrophs support a diverse assemblage of co-occurring bacteria and protozoa, with Methylophaga as key consumers of methane-derived organic matter. This study identifies the taxa contributing to the flow of seep-derived carbon through microbial mat biomass, revealing the bacterial and eukaryotic diversity of these remarkable ecosystems.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1379742

Journal Information:: Frontiers in Microbiology, Journal Name: Frontiers in Microbiology Journal Issue: FEB Vol. 8; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

References (77)

Oceanic Methane Biogeochemistry Reeburgh, William S. ChemInform, Vol. 38, Issue 20 https://doi.org/10.1002/chin.200720267	journal	May 2007
Aerobic Methylotrophic Prokaryotes Lidstrom, Mary E. The Prokaryotes https://doi.org/10.1007/0-387-30742-7_20	book	January 2006
Scanning electron microscopy coupled with energy-dispersive X-ray spectrometry for quick detection of sulfur-oxidizing bacteria in environmental water samples Sun, Chengjun; Jiang, Fenghua; Gao, Wei Chinese Journal of Oceanology and Limnology, Vol. 35, Issue 1 https://doi.org/10.1007/s00343-016-5175-1	journal	March 2016
Natural oil seepage at coal oil point, Santa Barbara, California Allen, Alan A.; Schlueter, Roger S.; Mikolaj, Paul G. Biological Conservation, Vol. 4, Issue 2 https://doi.org/10.1016/0006-3207(72)90008-0	journal	January 1972
Meiofauna and chlorophyll associated with Beggiatoa mats of a natural submarine petroleum seep Montagna, Paul A.; Spies, Robert B. Marine Environmental Research, Vol. 16, Issue 4 https://doi.org/10.1016/0141-1136(85)90021-2	journal	January 1985
Carbon and hydrogen isotope fractionation associated with the aerobic microbial oxidation of methane, ethane, propane and butane Kinnaman, Franklin S.; Valentine, David L.; Tyler, Stanley C. Geochimica et Cosmochimica Acta, Vol. 71, Issue 2 https://doi.org/10.1016/j.gca.2006.09.007	journal	January 2007
Structural diversity and fate of intact polar lipids in marine sediments Lipp, Julius Sebastian; Hinrichs, Kai-Uwe Geochimica et Cosmochimica Acta, Vol. 73, Issue 22 https://doi.org/10.1016/j.gca.2009.08.003	journal	November 2009
Stable carbon isotopic compositions of intact polar lipids reveal complex carbon flow patterns among hydrocarbon degrading microbial communities at the Chapopote asphalt volcano Schubotz, Florence; Lipp, Julius S.; Elvert, Marcus Geochimica et Cosmochimica Acta, Vol. 75, Issue 16 https://doi.org/10.1016/j.gca.2011.05.018	journal	August 2011
Biochemical degradation of algal fatty acids in oxic and anoxic sediment–seawater interface systems: effects of structural association and relative roles of aerobic and anaerobic bacteria Ding, Haibing; Sun, Ming-Yi Marine Chemistry, Vol. 93, Issue 1 https://doi.org/10.1016/j.marchem.2004.04.004	journal	January 2005
The spatial scales, distribution, and intensity of natural marine hydrocarbon seeps near Coal Oil Point, California Washburn, Libe; Clark, Jordan F.; Kyriakidis, Phaedon Marine and Petroleum Geology, Vol. 22, Issue 4 https://doi.org/10.1016/j.marpetgeo.2004.08.006	journal	April 2005
Relationships between sulfide-oxidizing bacterial mats and their carbon sources in northern Gulf of Mexico cold seeps Gilhooly, William P.; Carney, Robert S.; Macko, Stephen A. Organic Geochemistry, Vol. 38, Issue 3 https://doi.org/10.1016/j.orggeochem.2006.06.005	journal	March 2007
Microbial sources of intact polar diacylglycerolipids in the Western North Atlantic Ocean Popendorf, Kimberly J.; Lomas, Michael W.; Van Mooy, Benjamin A. S. Organic Geochemistry, Vol. 42, Issue 7 https://doi.org/10.1016/j.orggeochem.2011.05.003	journal	August 2011
Symbiont–host relationships in chemosynthetic mussels: A comprehensive lipid biomarker study Kellermann, Matthias Y.; Schubotz, Florence; Elvert, Marcus Organic Geochemistry, Vol. 43 https://doi.org/10.1016/j.orggeochem.2011.10.005	journal	February 2012
Basic local alignment search tool Altschul, Stephen F.; Gish, Warren; Miller, Webb Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410 https://doi.org/10.1016/s0022-2836(05)80360-2	journal	October 1990
Phospholipid analysis as a tool to study complex microbial communities in marine sediments Rütters, Heike; Sass, Henrik; Cypionka, Heribert Journal of Microbiological Methods, Vol. 48, Issue 2-3 https://doi.org/10.1016/s0167-7012(01)00319-0	journal	February 2002
Autonomous Marine Robotic Technology Reveals an Expansive Benthic Bacterial Community Relevant to Regional Nitrogen Biogeochemistry Valentine, David L.; Fisher, G. Burch; Pizarro, Oscar Environmental Science & Technology, Vol. 50, Issue 20 https://doi.org/10.1021/acs.est.6b03584	journal	October 2016
Disentangling Oil Weathering at a Marine Seep Using GC×GC: Broad Metabolic Specificity Accompanies Subsurface Petroleum Biodegradation Wardlaw, George D.; Arey, J. Samuel; Reddy, Christopher M. Environmental Science & Technology, Vol. 42, Issue 19 https://doi.org/10.1021/es8013908	journal	October 2008
Weathering and the Fallout Plume of Heavy Oil from Strong Petroleum Seeps Near Coal Oil Point, CA Farwell, Christopher; Reddy, Christopher M.; Peacock, Emily Environmental Science & Technology, Vol. 43, Issue 10 https://doi.org/10.1021/es802586g	journal	May 2009
Biogeography and phylogenetic diversity of a cluster of exclusively marine myxobacteria Brinkhoff, Thorsten; Fischer, Doreen; Vollmers, John The ISME Journal, Vol. 6, Issue 6 https://doi.org/10.1038/ismej.2011.190	journal	December 2011
Analysis of bacterial and archaeal diversity in coastal microbial mats using massive parallel 16S rRNA gene tag sequencing Bolhuis, Henk; Stal, Lucas J. The ISME Journal, Vol. 5, Issue 11 https://doi.org/10.1038/ismej.2011.52	journal	May 2011
Cyanobacterial reuse of extracellular organic carbon in microbial mats Stuart, Rhona K.; Mayali, Xavier; Lee, Jackson Z. The ISME Journal, Vol. 10, Issue 5 https://doi.org/10.1038/ismej.2015.180	journal	October 2015
Novel microbial communities of the Haakon Mosby mud volcano and their role as a methane sink Niemann, Helge; Lösekann, Tina; de Beer, Dirk Nature, Vol. 443, Issue 7113 https://doi.org/10.1038/nature05227	journal	October 2006
Phytoplankton in the ocean use non-phosphorus lipids in response to phosphorus scarcity Van Mooy, Benjamin A. S.; Fredricks, Helen F.; Pedler, Byron E. Nature, Vol. 458, Issue 7234 https://doi.org/10.1038/nature07659	journal	March 2009
Urban aerosols harbor diverse and dynamic bacterial populations Brodie, E. L.; DeSantis, T. Z.; Parker, J. P. M. Proceedings of the National Academy of Sciences, Vol. 104, Issue 1, p. 299-304 https://doi.org/10.1073/pnas.0608255104	journal	December 2006
Natural gas and temperature structured a microbial community response to the Deepwater Horizon oil spill Redmond, M. C.; Valentine, D. L. Proceedings of the National Academy of Sciences, Vol. 109, Issue 50 https://doi.org/10.1073/pnas.1108756108	journal	October 2011
Global dispersion and local diversification of the methane seep microbiome Ruff, S. Emil; Biddle, Jennifer F.; Teske, Andreas P. Proceedings of the National Academy of Sciences, Vol. 112, Issue 13 https://doi.org/10.1073/pnas.1421865112	journal	March 2015
Clustal W and Clustal X version 2.0 Larkin, M. A.; Blackshields, G.; Brown, N. P. Bioinformatics, Vol. 23, Issue 21, p. 2947-2948 https://doi.org/10.1093/bioinformatics/btm404	journal	September 2007
Bacterial diversity in marine hydrocarbon seep sediments LaMontagne, Michael G.; Leifer, Ira; Bergmann, Sandra Environmental Microbiology, Vol. 6, Issue 8 https://doi.org/10.1111/j.1462-2920.2004.00613.x	journal	August 2004
Detection of microbial biomass by intact polar membrane lipid analysis in the water column and surface sediments of the Black Sea Schubotz, Florence; Wakeham, Stuart G.; Lipp, Julius S. Environmental Microbiology, Vol. 11, Issue 10 https://doi.org/10.1111/j.1462-2920.2009.01999.x	journal	October 2009
Comparative microbial diversity analyses of modern marine thrombolitic mats by barcoded pyrosequencing: Microbial diversity in modern thrombolitic mats Mobberley, Jennifer M.; Ortega, Maya C.; Foster, Jamie S. Environmental Microbiology, Vol. 14, Issue 1 https://doi.org/10.1111/j.1462-2920.2011.02509.x	journal	June 2011
Bacteria and diatom co-occurrence patterns in microbial mats from polar desert streams: Diatom : bacteria co-occurrence in Dry Valley streams Stanish, Lee F.; O'Neill, Sean P.; Gonzalez, Antonio Environmental Microbiology, Vol. 15, Issue 4 https://doi.org/10.1111/j.1462-2920.2012.02872.x	journal	September 2012
Oleomonas sagaranensis gen. nov., sp. nov., represents a novel genus in the Î±- Proteobacteria Kanamori, Takeshi; Rashid, Naeem; Morikawa, Masaaki FEMS Microbiology Letters, Vol. 217, Issue 2 https://doi.org/10.1111/j.1574-6968.2002.tb11484.x	journal	December 2002
Starvation and recovery in the deep-sea methanotroph M ethyloprofundus sedimenti : Stress response in Tavormina, Patricia L.; Kellermann, Matthias Y.; Antony, Chakkiath Paul Molecular Microbiology, Vol. 103, Issue 2 https://doi.org/10.1111/mmi.13553	journal	November 2016
Deep-Sea Oil Plume Enriches Indigenous Oil-Degrading Bacteria Hazen, Terry C.; Dubinsky, Eric A.; DeSantis, Todd Z. Science, Vol. 330, Issue 6001 https://doi.org/10.1126/science.1195979	journal	August 2010
A Persistent Oxygen Anomaly Reveals the Fate of Spilled Methane in the Deep Gulf of Mexico Kessler, J. D.; Valentine, D. L.; Redmond, M. C. Science, Vol. 331, Issue 6015 https://doi.org/10.1126/science.1199697	journal	January 2011
A Methanotrophic Marine Molluscan (Bivalvia, Mytilidae) Symbiosis: Mussels Fueled by Gas Childress, J. J.; Fisher, C. R.; Brooks, J. M. Science, Vol. 233, Issue 4770 https://doi.org/10.1126/science.233.4770.1306	journal	September 1986
Diversity and Abundance of Aerobic and Anaerobic Methane Oxidizers at the Haakon Mosby Mud Volcano, Barents Sea Losekann, T.; Knittel, K.; Nadalig, T. Applied and Environmental Microbiology, Vol. 73, Issue 10 https://doi.org/10.1128/aem.00016-07	journal	March 2007
Identification of Novel Methane-, Ethane-, and Propane-Oxidizing Bacteria at Marine Hydrocarbon Seeps by Stable Isotope Probing Redmond, M. C.; Valentine, D. L.; Sessions, A. L. Applied and Environmental Microbiology, Vol. 76, Issue 19 https://doi.org/10.1128/aem.00271-10	journal	July 2010
New Screening Software Shows that Most Recent Large 16S rRNA Gene Clone Libraries Contain Chimeras Ashelford, K. E.; Chuzhanova, N. A.; Fry, J. C. Applied and Environmental Microbiology, Vol. 72, Issue 9 https://doi.org/10.1128/aem.00556-06	journal	September 2006
Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities Schloss, P. D.; Westcott, S. L.; Ryabin, T. Applied and Environmental Microbiology, Vol. 75, Issue 23, p. 7537-7541 https://doi.org/10.1128/aem.01541-09	journal	October 2009
Insights into the Diversity of Eukaryotes in Acid Mine Drainage Biofilm Communities Baker, B. J.; Tyson, G. W.; Goosherst, L. Applied and Environmental Microbiology, Vol. 75, Issue 7 https://doi.org/10.1128/aem.02500-08	journal	February 2009
Characterization of Large, Autotrophic Beggiatoa spp. Abundant at Hydrothermal Vents of the Guaymas Basin † Nelson, Douglas C.; Wirsen, Carl O.; Jannasch, Holger W. Applied and Environmental Microbiology, Vol. 55, Issue 11 https://doi.org/10.1128/aem.55.11.2909-2917.1989	journal	January 1989
Diversity and Distribution of Methanotrophic Archaea at Cold Seeps Knittel, K.; Losekann, T.; Boetius, A. Applied and Environmental Microbiology, Vol. 71, Issue 1 https://doi.org/10.1128/aem.71.1.467-479.2005	journal	January 2005
Subsurface Microbial Methanotrophic Mats in the Black Sea Treude, T.; Knittel, K.; Blumenberg, M. Applied and Environmental Microbiology, Vol. 71, Issue 10 https://doi.org/10.1128/aem.71.10.6375-6378.2005	journal	October 2005
In Vitro Study of Lipid Biosynthesis in an Anaerobically Methane-Oxidizing Microbial Mat Blumenberg, M.; Seifert, R.; Nauhaus, K. Applied and Environmental Microbiology, Vol. 71, Issue 8 https://doi.org/10.1128/aem.71.8.4345-4351.2005	journal	August 2005
Methanotrophic bacteria. Hanson, R. S.; Hanson, T. E. Microbiological reviews, Vol. 60, Issue 2 https://doi.org/10.1128/mr.60.2.439-471.1996	journal	January 1996
Temporal variability and the relationship between benthic meiofaunal and microbial populations of a natural coastal petroleum seep Montagna, Paul A.; Bauer, James E.; Toal, Jon Journal of Marine Research, Vol. 45, Issue 3 https://doi.org/10.1357/002224087788326894	journal	August 1987
Microbial Iron Mats at the Mid-Atlantic Ridge and Evidence that Zetaproteobacteria May Be Restricted to Iron-Oxidizing Marine Systems Scott, Jarrod J.; Breier, John A.; Luther, George W. PLOS ONE, Vol. 10, Issue 3 https://doi.org/10.1371/journal.pone.0119284	journal	March 2015
Role of microbial mats in Sulaibikhat Bay (Kuwait) mudflat food webs: evidence from δ13C analysis Al-Zaidan, Asy; Kennedy, H.; Jones, Da Marine Ecology Progress Series, Vol. 308 https://doi.org/10.3354/meps308027	journal	February 2006
Exopolysaccharides produced by marine bacteria and their applications as glycosaminoglycan-like molecules Delbarre-Ladrat, Christine; Sinquin, Corinne; Lebellenger, Lou Frontiers in Chemistry, Vol. 2 https://doi.org/10.3389/fchem.2014.00085	journal	October 2014
Intact polar membrane lipids in prokaryotes and sediments deciphered by high-performance liquid chromatography/electrospray ionization multistage mass spectrometry—new biomarkers for biogeochemistry and microbial ecology Sturt, Helen F.; Summons, Roger E.; Smith, Kristin Rapid Communications in Mass Spectrometry, Vol. 18, Issue 6 https://doi.org/10.1002/rcm.1378	journal	January 2004
The Methanotrophs — The Families Methylococcaceae and Methylocystaceae Bowman, John The Prokaryotes https://doi.org/10.1007/0-387-30745-1_15	book	January 2006
Compositional changes in natural gas bubble plumes: observations from the Coal Oil Point marine hydrocarbon seep field Clark, Jordan F.; Leifer, Ira; Washburn, Libe Geo-Marine Letters, Vol. 23, Issue 3-4 https://doi.org/10.1007/s00367-003-0137-y	journal	December 2003
Distribution of betaine lipids in marine algae Kato, Misako; Sakai, Miho; Adachi, Kyoko Phytochemistry, Vol. 42, Issue 5 https://doi.org/10.1016/0031-9422(96)00115-X	journal	July 1996
Basic local alignment search tool Altschul, Stephen F.; Gish, Warren; Miller, Webb Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410 https://doi.org/10.1016/S0022-2836(05)80360-2	journal	October 1990
Phospholipid analysis as a tool to study complex microbial communities in marine sediments Rütters, Heike; Sass, Henrik; Cypionka, Heribert Journal of Microbiological Methods, Vol. 48, Issue 2-3 https://doi.org/10.1016/S0167-7012(01)00319-0	journal	February 2002
Oceanic Methane Biogeochemistry Reeburgh, William S. Chemical Reviews, Vol. 107, Issue 2 https://doi.org/10.1021/cr050362v	journal	February 2007
The world's most spectacular marine hydrocarbon seeps (Coal Oil Point, Santa Barbara Channel, California): Quantification of emissions Hornafius, J. Scott; Quigley, Derek; Luyendyk, Bruce P. Journal of Geophysical Research: Oceans, Vol. 104, Issue C9 https://doi.org/10.1029/1999JC900148	journal	September 1999
Symbiosis of methylotrophic bacteria and deep-sea mussels Cavanaugh, Colleen M.; Levering, Piet R.; Maki, James S. Nature, Vol. 325, Issue 6102 https://doi.org/10.1038/325346a0	journal	January 1987
DNA stable-isotope probing Neufeld, Josh D.; Vohra, Jyotsna; Dumont, Marc G. Nature Protocols, Vol. 2, Issue 4 https://doi.org/10.1038/nprot.2007.109	journal	April 2007
Methyloprofundus sedimenti gen. nov., sp. nov., an obligate methanotroph from ocean sediment belonging to the 'deep sea-1' clade of marine methanotrophs Tavormina, P. L.; Hatzenpichler, R.; McGlynn, S. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 65, Issue Pt 1 https://doi.org/10.1099/ijs.0.062927-0	journal	October 2014
Bacterial and archaeal diversities in Yunnan and Tibetan hot springs, China: Microbial diversities in hot springs Song, Zhao-Qi; Wang, Feng-Ping; Zhi, Xiao-Yang Environmental Microbiology, Vol. 15, Issue 4 https://doi.org/10.1111/1462-2920.12025	journal	November 2012
Novel uncultured Epsilonproteobacteria dominate a filamentous sulphur mat from the 13Â°N hydrothermal vent field, East Pacific Rise: Novel uncultured Epsilonproteobacteria Moussard, HÃ©lÃ¨ne; Corre, Erwan; Cambon-Bonavita, Marie-Anne FEMS Microbiology Ecology, Vol. 58, Issue 3 https://doi.org/10.1111/j.1574-6941.2006.00192.x	journal	December 2006
Natural Oil Seepage at Coal Oil Point, Santa Barbara, California Allen, A. A.; Schlueter, R. S.; Mikolaj, P. G. Science, Vol. 170, Issue 3961 https://doi.org/10.1126/science.170.3961.974	journal	November 1970
Diversity and Abundance of Aerobic and Anaerobic Methane Oxidizers at the Haakon Mosby Mud Volcano, Barents Sea Losekann, T.; Knittel, K.; Nadalig, T. Applied and Environmental Microbiology, Vol. 73, Issue 10 https://doi.org/10.1128/AEM.00016-07	journal	March 2007
Identification of Novel Methane-, Ethane-, and Propane-Oxidizing Bacteria at Marine Hydrocarbon Seeps by Stable Isotope Probing Redmond, M. C.; Valentine, D. L.; Sessions, A. L. Applied and Environmental Microbiology, Vol. 76, Issue 19 https://doi.org/10.1128/AEM.00271-10	journal	July 2010
New Screening Software Shows that Most Recent Large 16S rRNA Gene Clone Libraries Contain Chimeras Ashelford, K. E.; Chuzhanova, N. A.; Fry, J. C. Applied and Environmental Microbiology, Vol. 72, Issue 9 https://doi.org/10.1128/AEM.00556-06	journal	September 2006
Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities Schloss, P. D.; Westcott, S. L.; Ryabin, T. Applied and Environmental Microbiology, Vol. 75, Issue 23, p. 7537-7541 https://doi.org/10.1128/AEM.01541-09	journal	October 2009
Insights into the Diversity of Eukaryotes in Acid Mine Drainage Biofilm Communities Baker, B. J.; Tyson, G. W.; Goosherst, L. Applied and Environmental Microbiology, Vol. 75, Issue 7 https://doi.org/10.1128/AEM.02500-08	journal	February 2009
Characterization of Large, Autotrophic Beggiatoa spp. Abundant at Hydrothermal Vents of the Guaymas Basin † Nelson, Douglas C.; Wirsen, Carl O.; Jannasch, Holger W. Applied and Environmental Microbiology, Vol. 55, Issue 11 https://doi.org/10.1128/AEM.55.11.2909-2917.1989	journal	January 1989
Diversity and Distribution of Methanotrophic Archaea at Cold Seeps Knittel, K.; Losekann, T.; Boetius, A. Applied and Environmental Microbiology, Vol. 71, Issue 1 https://doi.org/10.1128/AEM.71.1.467-479.2005	journal	January 2005
Subsurface Microbial Methanotrophic Mats in the Black Sea Treude, T.; Knittel, K.; Blumenberg, M. Applied and Environmental Microbiology, Vol. 71, Issue 10 https://doi.org/10.1128/AEM.71.10.6375-6378.2005	journal	October 2005
Lipid Biomarkers and Carbon Isotope Signatures of a Microbial (Beggiatoa) Mat Associated with Gas Hydrates in the Gulf of Mexico Zhang, C. L.; Huang, Z.; Cantu, J. Applied and Environmental Microbiology, Vol. 71, Issue 4 https://doi.org/10.1128/AEM.71.4.2106-2112.2005	journal	April 2005
Characteristics and Living Patterns of Marine Myxobacterial Isolates Zhang, Y. -q.; Li, Y. -z.; Wang, B. Applied and Environmental Microbiology, Vol. 71, Issue 6 https://doi.org/10.1128/AEM.71.6.3331-3336.2005	journal	June 2005
In Vitro Study of Lipid Biosynthesis in an Anaerobically Methane-Oxidizing Microbial Mat Blumenberg, M.; Seifert, R.; Nauhaus, K. Applied and Environmental Microbiology, Vol. 71, Issue 8 https://doi.org/10.1128/AEM.71.8.4345-4351.2005	journal	August 2005
Methanotrophic bacteria. Hanson, R. S.; Hanson, T. E. Microbiological reviews, Vol. 60, Issue 2 https://doi.org/10.1128/MMBR.60.2.439-471.1996	journal	January 1996
A laboratory experiment of intact polar lipid degradation in sandy sediments Logemann, J.; Graue, J.; Köster, J. Biogeosciences, Vol. 8, Issue 9 https://doi.org/10.5194/bg-8-2547-2011	journal	January 2011

Cited By (5)

Microbial megacities fueled by methane oxidation in a mineral spring cave Karwautz, Clemens; Kus, Günter; Stöckl, Michael The ISME Journal, Vol. 12, Issue 1 https://doi.org/10.1038/ismej.2017.146	journal	September 2017
In situ development of a methanotrophic microbiome in deep-sea sediments Ruff, S. E.; Felden, J.; Gruber-Vodicka, H. R. The ISME Journal, Vol. 13, Issue 1 https://doi.org/10.1038/s41396-018-0263-1	journal	August 2018
Possible cross-feeding pathway of facultative methylotroph Methyloceanibacter caenitepidi Gela4 on methanotroph Methylocaldum marinum S8 Takeuchi, Mio; Ozaki, Haruka; Hiraoka, Satoshi PLOS ONE, Vol. 14, Issue 3 https://doi.org/10.1371/journal.pone.0213535	journal	March 2019
Communal metabolism by Methylococcaceae and Methylophilaceae is driving rapid aerobic methane oxidation in sediments of a shallow seep near Elba, Italy Taubert, Martin; Grob, Carolina; Crombie, Andrew Environmental Microbiology, Vol. 21, Issue 10 https://doi.org/10.1111/1462-2920.14728	journal	July 2019
Novel facultative Methylocella strains are active methane consumers at terrestrial natural gas seeps Farhan Ul Haque, Muhammad; Crombie, Andrew T.; Murrell, J. Colin Microbiome, Vol. 7, Issue 1 https://doi.org/10.1186/s40168-019-0741-3	journal	October 2019

Similar Records

Comparative metagenomics of hydrocarbon and methane seeps of the Gulf of Mexico

Journal Article · Tue Nov 21 19:00:00 EST 2017 · Scientific Reports · OSTI ID:1543776

Carbonate-hosted microbial communities are prolific and pervasive methane oxidizers at geologically diverse marine methane seep sites

Journal Article · Sun Jun 13 20:00:00 EDT 2021 · Proceedings of the National Academy of Sciences of the United States of America · OSTI ID:1826336

Microbial sulfate reduction rates and sulfur and oxygen isotope fractionations at oil and gas seeps in deepwater Gulf of Mexico

Journal Article · Fri Dec 31 23:00:00 EST 1999 · Geochimica et Cosmochimica Acta · OSTI ID:20014546

Related Subjects

16S rRNA gene
54 ENVIRONMENTAL SCIENCES
59 BASIC BIOLOGICAL SCIENCES
intact polar lipids (IPL)
methanotrophs
microbial mats
stable isotope probing
sulfide-oxidizing bacteria

Methane-Oxidizing Bacteria Shunt Carbon to Microbial Mats at a Marine Hydrocarbon Seep

Citation Formats

References (77)

Cited By (5)

Similar Records

Related Subjects