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Title: Evaluation of Relationships between Growth Rate, Tree Size, Lignocellulose Composition, and Enzymatic Saccharification in Interspecific Corymbia Hybrids and Parental Taxa

Abstract

In order for a lignocellulosic bioenergy feedstock to be considered sustainable,it must possess a high rate of growth to supply biomass for conversion. Despite the desirability of a fast growth rate for industrial application,it is unclear what effect growth rate has on biomass composition or saccharification. We characterized Klason lignin,glucan,and xylan content with response to growth in Corymbia interspecific F1 hybrid families (HF) and parental species Corymbia torelliana and C. citriodora subspecies variegata and measured the effects on enzymatic hydrolysis from hydrothermally pretreated biomass. Analysis of biomass composition within Corymbia populations found similar amounts of Klason lignin content (19.7–21.3%) among parental and hybrid populations,whereas glucan content was clearly distinguished within C. citriodora subspecies variegata (52%) and HF148 (60%) as compared to other populations (28–38%). Multiple linear regression indicates that biomass composition is significantly impacted by tree size measured at the same age,with Klason lignin content increasing with diameter breast height (DBH) (+0.12% per cm DBH increase),and glucan and xylan typically decreasing per DBH cm increase (-0.7 and -0.3%,respectively). Polysaccharide content within C. citriodora subspecies variegata and HF-148 were not significantly affected by tree size. High-throughput enzymatic saccharification of hydrothermally pretreated biomass found significant differences among Corymbia populations for total glucosemore » production from biomass,with parental Corymbia torelliana and hybrids HF-148 and HF-51 generating the highest amounts of glucose (~180 mg/g biomass,respectively),with HF-51 undergoing the most efficient glucan-to-glucose conversion (74%). Based on growth rate,biomass composition,and further optimization of enzymatic saccharification yield,high production Corymbia hybrid trees are potentially suitable for fast-rotation bioenergy or biomaterial production.« less

Authors:
 [1];  [2];  [3];  [4];  [5];  [6];  [6];  [5];  [5];  [1]
  1. Univ. of Queensland, St. Lucia, QLD (Australia)
  2. Univ. of the Sunshine Coast, Marochydore, QLD (Australia); Dept. of Agriculture and Fisheries, Gympie, QLD (Australia)
  3. Prozess Technologie, St. Louis, MO (United States)
  4. Lawrence Berkeley National Lab. (LBNL), Emeryville, CA (United States)
  5. Lawrence Berkeley National Lab. (LBNL), Emeryville, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
  6. Univ. of Milan, Milan (Italy)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1379588
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Frontiers in Plant Science
Additional Journal Information:
Journal Volume: 7; Journal Issue: NOVEMBER2016; Journal ID: ISSN 1664-462X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; Corymbia; biofuels; eucalypt; saccharification; growth rate; lignin; glucan; xylan

Citation Formats

Healey, Adam L., Lee, David J., Lupoi, Jason S., Papa, Gabriella, Guenther, Joel M., Corno, Luca, Adani, Fabrizio, Singh, Seema, Simmons, Blake A., and Henry, Robert J. Evaluation of Relationships between Growth Rate, Tree Size, Lignocellulose Composition, and Enzymatic Saccharification in Interspecific Corymbia Hybrids and Parental Taxa. United States: N. p., 2016. Web. doi:10.3389/fpls.2016.01705.
Healey, Adam L., Lee, David J., Lupoi, Jason S., Papa, Gabriella, Guenther, Joel M., Corno, Luca, Adani, Fabrizio, Singh, Seema, Simmons, Blake A., & Henry, Robert J. Evaluation of Relationships between Growth Rate, Tree Size, Lignocellulose Composition, and Enzymatic Saccharification in Interspecific Corymbia Hybrids and Parental Taxa. United States. doi:10.3389/fpls.2016.01705.
Healey, Adam L., Lee, David J., Lupoi, Jason S., Papa, Gabriella, Guenther, Joel M., Corno, Luca, Adani, Fabrizio, Singh, Seema, Simmons, Blake A., and Henry, Robert J. Fri . "Evaluation of Relationships between Growth Rate, Tree Size, Lignocellulose Composition, and Enzymatic Saccharification in Interspecific Corymbia Hybrids and Parental Taxa". United States. doi:10.3389/fpls.2016.01705. https://www.osti.gov/servlets/purl/1379588.
@article{osti_1379588,
title = {Evaluation of Relationships between Growth Rate, Tree Size, Lignocellulose Composition, and Enzymatic Saccharification in Interspecific Corymbia Hybrids and Parental Taxa},
author = {Healey, Adam L. and Lee, David J. and Lupoi, Jason S. and Papa, Gabriella and Guenther, Joel M. and Corno, Luca and Adani, Fabrizio and Singh, Seema and Simmons, Blake A. and Henry, Robert J.},
abstractNote = {In order for a lignocellulosic bioenergy feedstock to be considered sustainable,it must possess a high rate of growth to supply biomass for conversion. Despite the desirability of a fast growth rate for industrial application,it is unclear what effect growth rate has on biomass composition or saccharification. We characterized Klason lignin,glucan,and xylan content with response to growth in Corymbia interspecific F1 hybrid families (HF) and parental species Corymbia torelliana and C. citriodora subspecies variegata and measured the effects on enzymatic hydrolysis from hydrothermally pretreated biomass. Analysis of biomass composition within Corymbia populations found similar amounts of Klason lignin content (19.7–21.3%) among parental and hybrid populations,whereas glucan content was clearly distinguished within C. citriodora subspecies variegata (52%) and HF148 (60%) as compared to other populations (28–38%). Multiple linear regression indicates that biomass composition is significantly impacted by tree size measured at the same age,with Klason lignin content increasing with diameter breast height (DBH) (+0.12% per cm DBH increase),and glucan and xylan typically decreasing per DBH cm increase (-0.7 and -0.3%,respectively). Polysaccharide content within C. citriodora subspecies variegata and HF-148 were not significantly affected by tree size. High-throughput enzymatic saccharification of hydrothermally pretreated biomass found significant differences among Corymbia populations for total glucose production from biomass,with parental Corymbia torelliana and hybrids HF-148 and HF-51 generating the highest amounts of glucose (~180 mg/g biomass,respectively),with HF-51 undergoing the most efficient glucan-to-glucose conversion (74%). Based on growth rate,biomass composition,and further optimization of enzymatic saccharification yield,high production Corymbia hybrid trees are potentially suitable for fast-rotation bioenergy or biomaterial production.},
doi = {10.3389/fpls.2016.01705},
journal = {Frontiers in Plant Science},
number = NOVEMBER2016,
volume = 7,
place = {United States},
year = {Fri Nov 18 00:00:00 EST 2016},
month = {Fri Nov 18 00:00:00 EST 2016}
}

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Works referenced in this record:

Repression of lignin biosynthesis promotes cellulose accumulation and growth in transgenic trees
journal, August 1999

  • Hu, Wen-Jing; Harding, Scott A.; Lung, Jrhau
  • Nature Biotechnology, Vol. 17, Issue 8, p. 808-812
  • DOI: 10.1038/11758

Inhibition of cellulases by phenols
journal, March 2010