Toxin-Antitoxin systems eliminate defective cells and preserve symmetry in B acillus subtilis biofilms: Defective cells and preserve symmetry in Bacillus subtilis biofilms

Bloom-Ackermann, Zohar; Steinberg, Nitai; Rosenberg, Gili; Oppenheimer-Shaanan, Yaara; Pollack, Dan; Ely, Shir; Storzi, Nimrod; Levy, Asaf; Kolodkin-Gal, Ilana

doi:10.1111/1462-2920.13471

Toxin-Antitoxin systems eliminate defective cells and preserve symmetry in B acillus subtilis biofilms: Defective cells and preserve symmetry in Bacillus subtilis biofilms

Journal Article · Thu Aug 25 00:00:00 EDT 2016 · Environmental Microbiology

DOI:https://doi.org/10.1111/1462-2920.13471· OSTI ID:1379576

Bloom-Ackermann, Zohar ^[1]; Steinberg, Nitai ^[1]; Rosenberg, Gili ^[1]; Oppenheimer-Shaanan, Yaara ^[1]; Pollack, Dan ^[1]; Ely, Shir ^[1]; Storzi, Nimrod ^[1]; Levy, Asaf ^[1]; Kolodkin-Gal, Ilana ^[1]

Weizmann Inst. of Science, Rehovot (Israel)

Toxin-antitoxin modules are gene pairs encoding a toxin and its antitoxin, and are found on the chromosomes of many bacteria, including pathogens. This paper characterizes the specific contribution of the TxpA and YqcG toxins in elimination of defective cells from developing Bacillus subtilis biofilms. On nutrient limitation, defective cells accumulated in the biofilm breaking its symmetry. Deletion of the toxins resulted in accumulation of morphologically abnormal cells, and interfered with the proper development of the multicellular community. Dual physiological responses are of significance for TxpA and YqcG activation: nitrogen deprivation enhances the transcription of both TxpA and YqcG toxins, and simultaneously sensitizes the biofilm cells to their activity. Furthermore, we demonstrate that while both toxins when overexpressed affect the morphology of the developing biofilm, the toxin TxpA can act to lyse and dissolve pre-established B. subtilis biofilms.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1379576

Journal Information:: Environmental Microbiology, Journal Name: Environmental Microbiology Journal Issue: 12 Vol. 18; ISSN 1462-2912

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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