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Title: Array-based detection of genetic alterations associated with disease

Abstract

The present invention relates to DNA sequences from regions of copy number change on chromosome 20. The sequences can be used in hybridization methods for the identification of chromosomal abnormalities associated with various diseases.

Inventors:
; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1379191
Patent Number(s):
9,753,035
Application Number:
11/900,648
Assignee:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA LBNL
DOE Contract Number:
AC03-76SF00098
Resource Type:
Patent
Resource Relation:
Patent File Date: 2007 Sep 11
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Pinkel, Daniel, Albertson, Donna G., and Gray, Joe W.. Array-based detection of genetic alterations associated with disease. United States: N. p., 2017. Web.
Pinkel, Daniel, Albertson, Donna G., & Gray, Joe W.. Array-based detection of genetic alterations associated with disease. United States.
Pinkel, Daniel, Albertson, Donna G., and Gray, Joe W.. 2017. "Array-based detection of genetic alterations associated with disease". United States. doi:. https://www.osti.gov/servlets/purl/1379191.
@article{osti_1379191,
title = {Array-based detection of genetic alterations associated with disease},
author = {Pinkel, Daniel and Albertson, Donna G. and Gray, Joe W.},
abstractNote = {The present invention relates to DNA sequences from regions of copy number change on chromosome 20. The sequences can be used in hybridization methods for the identification of chromosomal abnormalities associated with various diseases.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = 2017,
month = 9
}

Patent:

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  • The present invention relates to DNA sequences from regions of copy number change on chromosome 20. The sequences can be used in hybridization methods for the identification of chromosomal abnormalities associated with various diseases.
  • The present invention relates to a method and apparatus for selectively generating a next-in-time positional code for disconnectably connecting recording circuitry to different but contiguous sets of detectors, i.e. An ''active'' array, from among a plurality of detectors positioned along the line of survey. For this purpose a microcomputer system that includes a system bus connected (via a port) to the rollalong switch, is employed.
  • The aim of the present study was to detect complex genetic alterations in human glioblastoma multiforme (GBM) by comparative genomic in situ hybridization (CGH). Of the 24 GBM that were examined, increased fluorescence intensities indicating chromosomal polysomy of chromosome 7 and gene amplification at chromosome 7p were found in 42% of the tumors. In addition, signal enhancement of chromosome 19 was present in 29% and at 12q13-15 in 21% of the tumors. We also detected reduction of fluorescence intensities indicating gross deletions on chromosomes 10 (58%), 9p (46%), and 13 (29%). There was a close correlation of CGH results whenmore » compared with Southern analysis of the EGFR gene localized on chromosome 7 and loss of heterozygosity detection of chromosome 9 and 10 by microsatellite PCR. A close correlation was also observed between copy number changes of chromosome 7 and deletions of chromosome 10. Amplification of chromosome 12q and deletions of chromosomes 9p and 13 seemed to be complementary in the tumors investigated in the present study. 44 refs., 3 figs., 1 tab.« less
  • Methods and compositions for staining based upon nucleic acid sequence that employ nudeic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in themore » detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML), retinoblastoma, ovarian and uterine cancers, and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications.« less