Modular synthetic inverters from zinc finger proteins and small RNAs

Hsia, Justin; Holtz, William J.; Maharbiz, Michel M.; Arcak, Murat; Keasling, Jay D.; Rao, Christopher V.

doi:10.1371/journal.pone.0149483

Title: Modular synthetic inverters from zinc finger proteins and small RNAs

Journal Article · Wed Feb 17 00:00:00 EST 2016 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0149483· OSTI ID:1256959

Hsia, Justin ^[1]; Holtz, William J. ^[2]; Maharbiz, Michel M. ^[1]; Arcak, Murat ^[1]; Keasling, Jay D. ^[3]; Rao, Christopher V. ^[4]

Univ. of California, Berkeley, CA (United States)
Univ. of California, Berkeley, CA (United States); Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Univ. of Illinois, Champaign, IL (United States)

© 2016 Hsia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Synthetic zinc finger proteins (ZFPs) can be created to target promoter DNA sequences, repressing transcription. The binding of small RNA (sRNA) to ZFP mRNA creates an ultrasensitive response to generate higher effective Hill coefficients. Here we combined three "off the shelf" ZFPs and three sRNAs to create new modular inverters in E. col i and quantify their behavior using induction fold. We found a general ordering of the effects of the ZFPs and sRNAs on induction fold that mostly held true when combining these parts. We then attempted to construct a ring oscillator using our new inverters. Our chosen parts performed insufficiently to create oscillations, but we include future directions for improvement upon our work presented here.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231; FA9550-14-1-0089

OSTI ID:: 1256959

Alternate ID(s):: OSTI ID: 1379062

Journal Information:: PLoS ONE, Vol. 11, Issue 2; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 5 works

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Cited By (2)

Programmable biomolecular switches for rewiring flux in Escherichia coli Gao, Cong; Hou, Jianshen; Xu, Peng Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-11793-7	journal	August 2019
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59 BASIC BIOLOGICAL SCIENCES
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