Characterizing non-photochemical quenching in leaves through fluorescence lifetime snapshots

Sylak-Glassman, Emily J.; Zaks, Julia; Amarnath, Kapil; Leuenberger, Michelle; Fleming, Graham R.

doi:10.1007/s11120-015-0104-2

Title: Characterizing non-photochemical quenching in leaves through fluorescence lifetime snapshots

Journal Article · Thu Mar 12 00:00:00 EDT 2015 · Photosynthesis Research

DOI:https://doi.org/10.1007/s11120-015-0104-2· OSTI ID:1378941

Sylak-Glassman, Emily J. ^[1]; Zaks, Julia ^[2]; Amarnath, Kapil ^[3]; Leuenberger, Michelle ^[4]; Fleming, Graham R. ^[5]

Univ. of California, Berkeley, CA (United States). Dept. of Chemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division; IDA Science and Technology Policy Inst. (STPI), Washington, DC (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division; Univ. of California, Berkeley, CA (United States). Graduate Program in Applied Science and Technology; Agilent Technologies, Santa Clara, CA (United States)
Univ. of California, Berkeley, CA (United States). Dept. of Chemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division; Harvard Univ., Cambridge, MA (United States). FAS Center for Systems Biology
Univ. of California, Berkeley, CA (United States). Dept. of Chemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division
Univ. of California, Berkeley, CA (United States). Dept. of Chemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division; Univ. of California, Berkeley, CA (United States). Graduate Program in Applied Science and Technology

A technique is described to measure the fluorescence decay profiles of intact leaves during adaptation to high light and subsequent relaxation to dark conditions. We illustrate how to ensure that photosystem II reaction centers are closed and compare data for wild type Arabidopsis thaliana with conventional pulse-amplitude modulated (PAM) fluorescence measurements. Unlike PAM measurements, the lifetime measurements are not sensitive to photobleaching or chloroplast shielding, and the form of the fluorescence decay provides additional information to test quantitative models of excitation dynamics in intact leaves.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF)

DOE Contract Number:: AC02-05CH11231

OSTI ID:: 1378941

Journal Information:: Photosynthesis Research, Vol. 127, Issue 1; ISSN 0166-8595

Publisher:: Springer

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Arabidopsis thaliana
Chlorophyll
Fluorescence lifetime
Fluorescence yield
Non-photochemical quenching

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