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Title: Characterizing non-photochemical quenching in leaves through fluorescence lifetime snapshots

Abstract

A technique is described to measure the fluorescence decay profiles of intact leaves during adaptation to high light and subsequent relaxation to dark conditions. We illustrate how to ensure that photosystem II reaction centers are closed and compare data for wild type Arabidopsis thaliana with conventional pulse-amplitude modulated (PAM) fluorescence measurements. Unlike PAM measurements, the lifetime measurements are not sensitive to photobleaching or chloroplast shielding, and the form of the fluorescence decay provides additional information to test quantitative models of excitation dynamics in intact leaves.

Authors:
 [1];  [2];  [3];  [4];  [5]
  1. Univ. of California, Berkeley, CA (United States). Dept. of Chemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division; IDA Science and Technology Policy Inst. (STPI), Washington, DC (United States)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division; Univ. of California, Berkeley, CA (United States). Graduate Program in Applied Science and Technology; Agilent Technologies, Santa Clara, CA (United States)
  3. Univ. of California, Berkeley, CA (United States). Dept. of Chemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division; Harvard Univ., Cambridge, MA (United States). FAS Center for Systems Biology
  4. Univ. of California, Berkeley, CA (United States). Dept. of Chemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division
  5. Univ. of California, Berkeley, CA (United States). Dept. of Chemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division; Univ. of California, Berkeley, CA (United States). Graduate Program in Applied Science and Technology
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Science Foundation (NSF)
OSTI Identifier:
1378941
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article
Resource Relation:
Journal Name: Photosynthesis Research; Journal Volume: 127; Journal Issue: 1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Arabidopsis thaliana; Chlorophyll; Fluorescence lifetime; Fluorescence yield; Non-photochemical quenching

Citation Formats

Sylak-Glassman, Emily J., Zaks, Julia, Amarnath, Kapil, Leuenberger, Michelle, and Fleming, Graham R. Characterizing non-photochemical quenching in leaves through fluorescence lifetime snapshots. United States: N. p., 2015. Web. doi:10.1007/s11120-015-0104-2.
Sylak-Glassman, Emily J., Zaks, Julia, Amarnath, Kapil, Leuenberger, Michelle, & Fleming, Graham R. Characterizing non-photochemical quenching in leaves through fluorescence lifetime snapshots. United States. doi:10.1007/s11120-015-0104-2.
Sylak-Glassman, Emily J., Zaks, Julia, Amarnath, Kapil, Leuenberger, Michelle, and Fleming, Graham R. Thu . "Characterizing non-photochemical quenching in leaves through fluorescence lifetime snapshots". United States. doi:10.1007/s11120-015-0104-2.
@article{osti_1378941,
title = {Characterizing non-photochemical quenching in leaves through fluorescence lifetime snapshots},
author = {Sylak-Glassman, Emily J. and Zaks, Julia and Amarnath, Kapil and Leuenberger, Michelle and Fleming, Graham R.},
abstractNote = {A technique is described to measure the fluorescence decay profiles of intact leaves during adaptation to high light and subsequent relaxation to dark conditions. We illustrate how to ensure that photosystem II reaction centers are closed and compare data for wild type Arabidopsis thaliana with conventional pulse-amplitude modulated (PAM) fluorescence measurements. Unlike PAM measurements, the lifetime measurements are not sensitive to photobleaching or chloroplast shielding, and the form of the fluorescence decay provides additional information to test quantitative models of excitation dynamics in intact leaves.},
doi = {10.1007/s11120-015-0104-2},
journal = {Photosynthesis Research},
number = 1,
volume = 127,
place = {United States},
year = {Thu Mar 12 00:00:00 EDT 2015},
month = {Thu Mar 12 00:00:00 EDT 2015}
}