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Title: Foot-and-Mouth Disease Virus-Associated Abortion and Vertical Transmission following Acute Infection in Cattle under Natural Conditions

Journal Article · · PLoS ONE
 [1];  [1];  [1];  [2];  [3];  [4];  [1];  [5]
  1. ICAR- Directorate of Foot and Mouth Disease, Mukteshwar, Nainital, Uttarakhand (India)
  2. Indian Veterinary Research Inst. (IVRI), Izatnagar, Bareilly, Uttar Pradesh (India). Centre for Animal Disease Research and Diagnosis (CADRAD)
  3. US Dept. of Agriculture (USDA), Greenport, NY (United States). Agricultural Research Service, Plum Island Animal Disease Center, Foreign Animal Disease Research Unit
  4. US Dept. of Agriculture (USDA), Greenport, NY (United States). Agricultural Research Service, Plum Island Animal Disease Center, Foreign Animal Disease Research Unit; Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)
  5. Foreign Animal Disease Research Unit, USDA-ARS, Plum Island Animal Disease Center, Greenport, New York (United States)
+ Show Author Affiliations

Foot-and-mouth disease (FMD) is a highly contagious and economically important viral disease of cloven-hoofed animals, including domestic and wild host species. During recent FMD outbreaks in India, spontaneous abortions were reported amongst FMD-affected and asymptomatic cows. The current study was an opportunistic investigation of these naturally occurring bovine abortions to assess causality of abortion and vertical transmission of FMDV from infected cows to fetuses. For this purpose, fetal tissue samples of eight abortuses (heart, liver, kidney, spleen, palatine tonsil, umbilical cord, soft palate, tongue, lungs, and submandibular lymph node) were collected and screened by various detection methods, including viral genome detection, virus isolation, and immunomicroscopy. Amongst these cases, gross pathological changes were observed in 3 abortuses. Gross pathological findings included blood-tinged peritoneal and pleural effusions and myocarditis. Hearts of infected calves had mild to moderate degeneration and necrosis of the myocardium with moderate infiltration by mixed inflammatory cells. Localization of FMDV antigen was demonstrated in lungs and soft palate by immunomicroscopy. FMDV serotype O viral genome was recovered from 7 of 8 cases. Infectious FMDV serotype O was rescued by chemical transfection of the total RNA extracted from three soft palate samples and was sequenced to confirm 100% identity of the VP1 (capsid) coding region with isolates collected from infected cattle during the acute phase of infection. Based upon these findings, it may be concluded that FMDV-associated abortion occurred among the infected pregnant cows included within this study and FMDV was subsequently transmitted vertically to fetuses. This is the first documentation of FMDV-associated abortions in cattle.

Research Organization:
US Dept. of Agriculture (USDA), Greenport, NY (United States). Agricultural Research Service
Sponsoring Organization:
USDOE; USDA
Grant/Contract Number:
1940-32000-057-00D; HSHQDC-12-X-0060
OSTI ID:
1378471
Journal Information:
PLoS ONE, Vol. 11, Issue 12; ISSN 1932-6203
Publisher:
Public Library of ScienceCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 16 works
Citation information provided by
Web of Science

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Cited By (4)

Development and evaluation of serotype-specific recombinase polymerase amplification combined with lateral flow dipstick assays for the diagnosis of foot-and-mouth disease virus serotype A, O and Asia1 journal November 2018
Knowledge and perception of pastoral community members about brucellosis as a cause of abortion in animals and its zoonotic importance in Amibara district, Afar Region, Ethiopia journal November 2018
First detection of foot-and-mouth disease virus O/Ind-2001d in Vietnam journal June 2017
Knowledge and behavior of cattle and sheep owners and herders regarding foot-and-mouth disease in Northern Algeria journal August 2019

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