The role of Rnf in ion gradient formation in Desulfovibrio alaskensis

Wang, Luyao; Bradstock, Peter; Li, Chuang; McInerney, Michael J.; Krumholz, Lee R.

doi:10.7717/peerj.1919

Title: The role of Rnf in ion gradient formation in Desulfovibrio alaskensis

Journal Article · Thu Apr 14 00:00:00 EDT 2016 · PeerJ

DOI:https://doi.org/10.7717/peerj.1919· OSTI ID:1375918

Wang, Luyao ^[1]; Bradstock, Peter ^[1]; Li, Chuang ^[1]; McInerney, Michael J. ^[1]; Krumholz, Lee R. ^[1]

Univ. of Oklahoma, Norman, OK (United States)

Rnf is a membrane protein complex that has been shown to be important in energy conservation. Here, Desulfovibrio alaskensis G20 and Rnf mutants of G20 were grown with different electron donor and acceptor combinations to determine the importance of Rnf in energy conservation and the type of ion gradient generated. The addition of the protonophore TCS strongly inhibited lactate-sulfate dependent growth whereas the sodium ionophore ETH2120 had no effect, indicating a role for the proton gradient during growth. Mutants in rnfA and rnfD were more sensitive to the protonophore at 5 µM than the parental strain, suggesting the importance of Rnf in the generation of a proton gradient. The electrical potential (ΔΨ), ΔpH and proton motive force were lower in thernfAmutant than in the parental strain of D.alaskensis G20. In conclusion, these results provide evidence that the Rnf complex in D. alaskensis functions as a primary proton pump whose activity is important for growth.

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Research Organization:: Office of Scientific and Technical Information, Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI ID:: 1375918

Journal Information:: PeerJ, Vol. 4; ISSN 2167-8359

Publisher:: PeerJ Inc.Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 6 works

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59 BASIC BIOLOGICAL SCIENCES
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energetics

Title: The role of Rnf in ion gradient formation in Desulfovibrio alaskensis

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