An RNA Aptamer Capable of Forming a Hydrogel by Self-Assembly

Huang, Zhen; Kangovi, Gagan N.; Wen, Wei; Lee, Sangwoo; Niu, Li

doi:10.1021/acs.biomac.7b00314

Title: An RNA Aptamer Capable of Forming a Hydrogel by Self-Assembly

Journal Article · Tue Jun 13 00:00:00 EDT 2017 · Biomacromolecules

DOI:https://doi.org/10.1021/acs.biomac.7b00314· OSTI ID:1374625

Huang, Zhen ^[1]; Kangovi, Gagan N. ^[2]; Wen, Wei ^[1]; Lee, Sangwoo ^[2];

^[1]

Univ. at Albany, State Univ. of New York, NY (United States)
Rensselaer Polytechnic Inst., Troy, NY (United States)

Hydrogels are supramolecular assemblies with both solute transport properties like liquids and mechanical properties like elastomers. To date, every type of biomolecules except ribonucleic acid (RNA), is capable of forming a hydrogel. Here, we report an RNA that forms a hydrogel by self-assembly. This RNA is originally identified by systematic evolution of ligands by exponential enrichment (SELEX) to enhance the activity of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors as a potential RNA drug for the treatment of cognitive disorders. The RNA hydrogel exhibits an elastic modulus plateau on the order of 102 Pa and shows dynamic RNA chain interactions with relaxation behaviors similar to living wormlike micellar solutions. Small-angle X-ray scattering and cryogenic electron microscopy characterization support the RNA network structures. Here, by sequence mutation and rheological measurements, we reveal two key sequence motifs in the RNA responsible for intermolecular recognition and the formation of a polymer network by self-assembly.

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Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC); NIH/NINDS; USDOD

Grant/Contract Number:: AC02-06CH11357; RO1 NS060812; W1XWH-09-1-0568

OSTI ID:: 1374625

Journal Information:: Biomacromolecules, Vol. 18, Issue 7; ISSN 1525-7797

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 7 works

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59 BASIC BIOLOGICAL SCIENCES
self organization
peptides and proteins
genetics
chemical structure hydrogels
hydrogel
RNA aptamers
AMPA receptors
polymer
SELEX

Title: An RNA Aptamer Capable of Forming a Hydrogel by Self-Assembly

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References (59)

Cited By (2)

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