An RNA Aptamer Capable of Forming a Hydrogel by Self-Assembly

Huang, Zhen; Kangovi, Gagan N.; Wen, Wei; Lee, Sangwoo; Niu, Li

doi:10.1021/acs.biomac.7b00314

An RNA Aptamer Capable of Forming a Hydrogel by Self-Assembly

Journal Article · Tue Jun 13 00:00:00 EDT 2017 · Biomacromolecules

DOI:https://doi.org/10.1021/acs.biomac.7b00314· OSTI ID:1374625

Huang, Zhen ^[1]; Kangovi, Gagan N. ^[2]; Wen, Wei ^[1]; Lee, Sangwoo ^[2]; ^[1]

Univ. at Albany, State Univ. of New York, NY (United States)
Rensselaer Polytechnic Inst., Troy, NY (United States)

Hydrogels are supramolecular assemblies with both solute transport properties like liquids and mechanical properties like elastomers. To date, every type of biomolecules except ribonucleic acid (RNA), is capable of forming a hydrogel. Here, we report an RNA that forms a hydrogel by self-assembly. This RNA is originally identified by systematic evolution of ligands by exponential enrichment (SELEX) to enhance the activity of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors as a potential RNA drug for the treatment of cognitive disorders. The RNA hydrogel exhibits an elastic modulus plateau on the order of 102 Pa and shows dynamic RNA chain interactions with relaxation behaviors similar to living wormlike micellar solutions. Small-angle X-ray scattering and cryogenic electron microscopy characterization support the RNA network structures. Here, by sequence mutation and rheological measurements, we reveal two key sequence motifs in the RNA responsible for intermolecular recognition and the formation of a polymer network by self-assembly.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: DOD; NIH/NINDS; USDOE Office of Science (SC)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1374625

Journal Information:: Biomacromolecules, Journal Name: Biomacromolecules Journal Issue: 7 Vol. 18; ISSN 1525-7797

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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59 BASIC BIOLOGICAL SCIENCES
RNA aptamers
AMPA receptors
SELEX
chemical structure hydrogels
genetics
hydrogel
peptides and proteins
polymer
self organization

An RNA Aptamer Capable of Forming a Hydrogel by Self-Assembly

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References (59)

Cited By (2)

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