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Title: Extra Large G-Protein Interactome Reveals Multiple Stress Response Function and Partner-Dependent XLG Subcellular Localization

Journal Article · · Frontiers in Plant Science
 [1];  [2];  [3]
  1. Northwest A&F Univ., Xianyang (China). College of Natural Resources and Environment; Univ. of North Carolina, Chapel Hill, NC (United States). Dept. of Biology
  2. Northwest A&F Univ., Xianyang (China). College of Natural Resources and Environment
  3. Univ. of North Carolina, Chapel Hill, NC (United States). Dept. of Biology and Dept. of Pharmacology
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The three-member family of Arabidopsis extra-large G proteins (XLG1-3) defines the prototype of an atypical Ga subunit in the heterotrimeric G protein complex. Some recent evidence indicate that XLG subunits operate along with its Gbg dimer in root morphology, stress responsiveness, and cytokinin induced development, however downstream targets of activated XLG proteins in the stress pathways are rarely known. In order to assemble a set of candidate XLG-targeted proteins, a yeast two-hybrid complementation-based screen was performed using XLG protein baits to query interactions between XLG and partner protein found in glucose-treated seedlings, roots, and Arabidopsis cells in culture. Seventy two interactors were identified and >60% of a test set displayed in vivo interaction with XLG proteins. Gene co-expression analysis shows that >70% of the interactors are positively correlated with the corresponding XLG partners. Gene Ontology enrichment for all the candidates indicates stress responses and posits a molecular mechanism involving a specific set of transcription factor partners to XLG. Genes encoding two of these transcription factors, SZF1 and 2, require XLG proteins for full NaCl-induced expression. Furthermore, the subcellular localization of the XLG proteins in the nucleus, endosome, and plasma membrane is dependent on the specific interacting partner.

Research Organization:
University of North Carolina, Chapel Hill, NC (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
Grant/Contract Number:
FG02-05ER15671; R01GM065989; MCB-0718202
OSTI ID:
1374569
Journal Information:
Frontiers in Plant Science, Vol. 8; ISSN 1664-462X
Publisher:
Frontiers Research FoundationCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 24 works
Citation information provided by
Web of Science

References (55)

A Reevaluation of the Role of the Heterotrimeric G Protein in Coupling Light Responses in Arabidopsis journal April 2003
G-Protein Complex Mutants Are Hypersensitive to Abscisic Acid Regulation of Germination and Postgermination Development journal March 2006
Extra-Large G Proteins Expand the Repertoire of Subunits in Arabidopsis Heterotrimeric G Protein Signaling journal July 2015
The role of PLDα1 in providing specificity to signal-response coupling by heterotrimeric G-protein components in Arabidopsis journal April 2016
Abscisic acid signal transduction in guard cells is mediated by phospholipase D activity journal October 1999
SPX1 is a phosphate-dependent inhibitor of PHOSPHATE STARVATION RESPONSE 1 in Arabidopsis journal September 2014
Colocalization of fluorescent markers in confocal microscope images of plant cells journal March 2008
The α -Subunit of the Arabidopsis Heterotrimeric G Protein, GPA1, Is a Regulator of Transpiration Efficiency journal March 2010
The heterotrimeric G-protein β subunit, AGB1, plays multiple roles in the A rabidopsis salinity response: G-protein subunit AGB1 functions in Na + homeostasis journal May 2015
Evidence for an unusual transmembrane configuration of AGG3, a class C Gγ subunit of Arabidopsis journal December 2014
The Many Faces of G Protein Signaling journal January 1998
Phosphatidic acid binding inhibits RGS1 activity to affect specific signaling pathways in Arabidopsis journal March 2017
Investigations on N-rich protein (NRP) of Arabidopsis thaliana under different stress conditions journal March 2011
The Endosomal Protein CHARGED MULTIVESICULAR BODY PROTEIN1 Regulates the Autophagic Turnover of Plastids in Arabidopsis journal February 2015
An empirical framework for binary interactome mapping journal December 2008
EXTRA-LARGE G PROTEINs Interact with E3 Ligases PUB4 and PUB2 and Function in Cytokinin and Developmental Processes journal December 2016
A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae journal August 2001
Mammalian G Proteins and Their Cell Type Specific Functions journal October 2005
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method journal December 2001
G Protein Activation without a GEF in the Plant Kingdom journal June 2012
A Central Regulatory System Largely Controls Transcriptional Activation and Repression Responses to Phosphate Starvation in Arabidopsis journal September 2010
T-DNA insertion mutants reveal complex expression patterns of the aldehyde dehydrogenase 3H1 locus in Arabidopsis thaliana journal March 2012
A G-Protein β Subunit, AGB1, Negatively Regulates the ABA Response and Drought Tolerance by Down-Regulating AtMPK6-Related Pathway in Arabidopsis journal January 2015
The ESCRT-Related CHMP1A and B Proteins Mediate Multivesicular Body Sorting of Auxin Carriers in Arabidopsis and Are Required for Plant Development journal March 2009
The CCCH-Type Zinc Finger Proteins AtSZF1 and AtSZF2 Regulate Salt Stress Responses in Arabidopsis journal June 2007
Arabidopsis G‐protein interactome reveals connections to cell wall carbohydrates and morphogenesis journal January 2011
EARLY RESPONSIVE TO DEHYDRATION 15 , a Negative Regulator of Abscisic Acid Responses in Arabidopsis journal October 2006
Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis journal June 2007
Arabidopsis extra-large G proteins (XLGs) regulate root morphogenesis: XLGs negatively regulate root growth journal October 2007
Saltational evolution of the heterotrimeric G protein signaling mechanisms in the plant kingdom journal September 2016
ERD15—An attenuator of plant ABA responses and stomatal aperture journal January 2012
Heterotrimeric G protein activation by G-protein-coupled receptors journal January 2008
Aldehyde Dehydrogenases in Arabidopsis thaliana: Biochemical Requirements, Metabolic Pathways, and Functional Analysis journal January 2011
Aldehyde dehydrogenase (ALDH) superfamily in plants: gene nomenclature and comparative genomics journal September 2012
Novel ABA- and dehydration-inducible aldehyde dehydrogenase genes isolated from the resurrection plant Craterostigma plantagineum and Arabidopsis thaliana: Novel stress-inducible aldehyde dehydrogenases journal December 2001
The β-Subunit of the Arabidopsis G Protein Negatively Regulates Auxin-Induced Cell Division and Affects Multiple Developmental Processes journal January 2003
Heterotrimeric G protein α and β subunits antagonistically modulate stomatal density in Arabidopsis thaliana journal December 2008
Rice DEP1, encoding a highly cysteine-rich G protein γ subunit, confers cadmium tolerance on yeast cells and plants journal October 2013
Subcellular Localization and Functional Domain Studies of DEFECTIVE KERNEL1 in Maize and Arabidopsis Suggest a Model for Aleurone Cell Fate Specification Involving CRINKLY4 and SUPERNUMERARY ALEURONE LAYER1 journal October 2007
Overexpression of the regulator of G-protein signalling protein enhances ABA-mediated inhibition of root elongation and drought tolerance in Arabidopsis journal January 2006
BiNGO: a Cytoscape plugin to assess overrepresentation of Gene Ontology categories in Biological Networks journal June 2005
Transcriptional Coordination of the Metabolic Network in Arabidopsis journal August 2006
A Seven-Transmembrane RGS Protein That Modulates Plant Cell Proliferation journal September 2003
Membrane-Localized Extra-Large G Proteins and Gβγ of the Heterotrimeric G Proteins Form Functional Complexes Engaged in Plant Immunity in Arabidopsis journal January 2015
Growth attenuation under saline stress is mediated by the heterotrimeric G protein complex journal January 2014
An atypical heterotrimeric G-protein γ-subunit is involved in guard cell K+-channel regulation and morphological development in Arabidopsis thaliana: Identification of a novel class of G-protein γ-subunits journal June 2011
Heterotrimeric G Protein Signaling in Filamentous Fungi journal October 2007
Heterotrimeric G protein signalling in the plant kingdom journal March 2013
Yeast two-hybrid: State of the art journal October 1999
d -Glucose sensing by a plasma membrane regulator of G signaling protein, At RGS1 journal September 2008
Ca 2+ -dependent GTPase, Extra-large G Protein 2 (XLG2), Promotes Activation of DNA-binding Protein Related to Vernalization 1 (RTV1), Leading to Activation of Floral Integrator Genes and Early Flowering in Arabidopsis journal January 2012
Phospholipase D Is a Negative Regulator of Proline Biosynthesis in Arabidopsis thaliana journal January 2004
Co-Expression Among Constituents of a Motif in the Protein–Protein Interaction Network journal February 2009
Transcriptome and de novo analysis of Rosa xanthina f. spontanea in response to cold stress journal October 2021
Arabidopsis heterotrimeric G proteins regulate immunity by directly coupling to the FLS2 receptor journal April 2016

Cited By (4)

Multi-functionality of proteins involved in GPCR and G protein signaling: making sense of structure–function continuum with intrinsic disorder-based proteoforms journal August 2019
Development and cell death domain-containing asparagine-rich protein (DCD/NRP): an essential protein in plant development and stress responses journal December 2018
GCR1 and GPA1 coupling regulates nitrate, cell wall, immunity and light responses in Arabidopsis journal April 2019
Genetic and Systematic Approaches Toward G Protein-Coupled Abiotic Stress Signaling in Plants journal September 2018

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
arabidopsis
extra-large G protein
XLG protein interactome
salt stress
SZF
yeast two hybrid
NaCl