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Altering carbon allocation in hybrid poplar ( Populus alba × grandidentata ) impacts cell wall growth and development

Journal Article · · Plant Biotechnology Journal
DOI:https://doi.org/10.1111/pbi.12682· OSTI ID:1374538
 [1];  [2];  [3];  [2];  [4]
  1. Univ. of British Columbia, Vancouver, BC (Canada). Dept. of Wood Science; University of British Columbia
  2. Univ. of Wisconsin, Madison, WI (United States). Dept. of Biochemistry; Wisconsin Energy Inst., Madison, WI (United States). Dept. of Energy Great Lakes Bioenergy Research Center
  3. Agricultural Research Council, Pretoria (South Africa). Biotechnology Platform
  4. Univ. of British Columbia, Vancouver, BC (Canada). Dept. of Wood Science; Wisconsin Energy Inst., Madison, WI (United States). Dept. of Energy Great Lakes Bioenergy Research Center
+ Show Author Affiliations
Galactinol synthase is a pivotal enzyme involved in the synthesis of the raffinose family of oligosaccharides (RFOs) that function as transport carbohydrates in the phloem, as storage compounds in sink tissues and as soluble metabolites that combat both abiotic and biotic stress in several plant species. For hybrid poplar (Populus alba 9 grandidentata) overexpressing the Arabidopsis thaliana GolS3 (AtGolS3) gene showed clear effects on development; the extreme overexpressing lines were stunted and had cell wall traits characteristic of tension wood, whereas lines with only moderate up-regulation grew normally and had moderately altered secondary cell wall composition and ultrastructure. Stem cross-sections of the developing xylem revealed a significant increase in the number of vessels, as well as the clear presence of a G-layer in the fibres. Furthermore, AtGolS3-OE lines possessed higher cellulose and lower lignin contents, an increase in cellulose crystallinity, and significantly altered hemicellulose-derived carbohydrates, notably manifested by their mannose and xylose contents. Additionally, the transgenic plants displayed elevated xylem starch content. Transcriptome interrogation of the transgenic plants showed a significant up-regulation of genes involved in the synthesis of myo-inositol, along with genes involved in sucrose degradation. Our results suggest that the over expression of GolS and its product galactinol may serve as a molecular signal that initiates metabolic changes, culminating in a change in cell wall development and potentially the formation of tension wood.
Research Organization:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Grant/Contract Number:
FC02-07ER64494
OSTI ID:
1374538
Journal Information:
Plant Biotechnology Journal, Journal Name: Plant Biotechnology Journal Journal Issue: 7 Vol. 15; ISSN 1467-7644
Publisher:
Society for Experimental Biology; Association of Applied BiologyCopyright Statement
Country of Publication:
United States
Language:
English

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Maize HSFA 2 and HSBP 2 antagonistically modulate raffinose biosynthesis and heat tolerance in Arabidopsis journal July 2019

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
RFOs
cellulose
galactinol synthase
lignin
sugar signalling
tension wood