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Title: Spatial mapping and profiling of metabolite distributions during germination

Abstract

Germination is a highly complex process by which seeds begin to develop and establish themselves as viable organisms. In this paper, we utilize a combination of GC-MS, LC-fluorescence, and mass spectrometry imaging (MSI) approaches to profile and visualize the metabolic distributions of germinating seeds from two different inbreds of maize seeds, B73 and Mo17. GC and LC analyses demonstrate that the two inbreds are highly differentiated in their metabolite profiles throughout the course of germination, especially with regard to amino acids, sugar alcohols, and small organic acids. Crude dissection of the seed followed by GC-MS analysis of polar metabolites also revealed that many compounds were highly sequestered among the various seed tissue types. To further localize compounds, matrix-assisted laser desorption/ionization MSI is utilized to visualize compounds in fine detail in their native environments over the course of germination. Most notably, the fatty acyl chain-dependent differential localization of phospholipids and TAGs were observed within the embryo and radicle, showing correlation with the heterogeneous distribution of fatty acids. Furthermore, other interesting observations include unusual localization of ceramides on the endosperm/scutellum boundary, and subcellular localization of ferulate in the aleurone.

Authors:
 [1];  [1];  [2];  [1];  [2];  [1];  [1]
  1. Ames Lab. and Iowa State Univ., Ames, IA (United States)
  2. Iowa State Univ., Ames, IA (United States)
Publication Date:
Research Org.:
Ames Lab., Ames, IA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1371899
Report Number(s):
IS-J-9341
Journal ID: ISSN 0032-0889
Grant/Contract Number:
AC02-07CH11358
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Plant Physiology (Bethesda)
Additional Journal Information:
Journal Name: Plant Physiology (Bethesda); Journal Volume: 174; Journal Issue: 3; Journal ID: ISSN 0032-0889
Publisher:
American Society of Plant Biologists
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; amino acids; B73; ceramides; ferulate; germination; maize; mass spectrometry imaging; metabolomics; Mo17; phospholipids

Citation Formats

Feenstra, Adam D., Alexander, Liza E., Song, Zhihong, Korte, Andrew R., Yandeau-Nelson, Marna D., Nikolau, Basil J., and Lee, Young -Jin. Spatial mapping and profiling of metabolite distributions during germination. United States: N. p., 2017. Web. doi:10.1104/pp.17.00652.
Feenstra, Adam D., Alexander, Liza E., Song, Zhihong, Korte, Andrew R., Yandeau-Nelson, Marna D., Nikolau, Basil J., & Lee, Young -Jin. Spatial mapping and profiling of metabolite distributions during germination. United States. doi:10.1104/pp.17.00652.
Feenstra, Adam D., Alexander, Liza E., Song, Zhihong, Korte, Andrew R., Yandeau-Nelson, Marna D., Nikolau, Basil J., and Lee, Young -Jin. Tue . "Spatial mapping and profiling of metabolite distributions during germination". United States. doi:10.1104/pp.17.00652. https://www.osti.gov/servlets/purl/1371899.
@article{osti_1371899,
title = {Spatial mapping and profiling of metabolite distributions during germination},
author = {Feenstra, Adam D. and Alexander, Liza E. and Song, Zhihong and Korte, Andrew R. and Yandeau-Nelson, Marna D. and Nikolau, Basil J. and Lee, Young -Jin},
abstractNote = {Germination is a highly complex process by which seeds begin to develop and establish themselves as viable organisms. In this paper, we utilize a combination of GC-MS, LC-fluorescence, and mass spectrometry imaging (MSI) approaches to profile and visualize the metabolic distributions of germinating seeds from two different inbreds of maize seeds, B73 and Mo17. GC and LC analyses demonstrate that the two inbreds are highly differentiated in their metabolite profiles throughout the course of germination, especially with regard to amino acids, sugar alcohols, and small organic acids. Crude dissection of the seed followed by GC-MS analysis of polar metabolites also revealed that many compounds were highly sequestered among the various seed tissue types. To further localize compounds, matrix-assisted laser desorption/ionization MSI is utilized to visualize compounds in fine detail in their native environments over the course of germination. Most notably, the fatty acyl chain-dependent differential localization of phospholipids and TAGs were observed within the embryo and radicle, showing correlation with the heterogeneous distribution of fatty acids. Furthermore, other interesting observations include unusual localization of ceramides on the endosperm/scutellum boundary, and subcellular localization of ferulate in the aleurone.},
doi = {10.1104/pp.17.00652},
journal = {Plant Physiology (Bethesda)},
number = 3,
volume = 174,
place = {United States},
year = {Tue Jun 20 00:00:00 EDT 2017},
month = {Tue Jun 20 00:00:00 EDT 2017}
}

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