Localizing Exciton Recombination Sites in Conformationally Distinct Single Conjugated Polymers by Super-resolution Fluorescence Imaging

Park, Heungman; Hoang, Dat Tien; Paeng, Keewook; Kaufman, Laura J.

doi:10.1021/acsnano.5b00086

Localizing Exciton Recombination Sites in Conformationally Distinct Single Conjugated Polymers by Super-resolution Fluorescence Imaging

Journal Article · Fri Mar 06 00:00:00 EST 2015 · ACS Nano

DOI:https://doi.org/10.1021/acsnano.5b00086· OSTI ID:1370377

Park, Heungman ^[1]; Hoang, Dat Tien ^[1]; Paeng, Keewook ^[2]; Kaufman, Laura J. ^[1]

Columbia Univ., New York, NY (United States)
Columbia Univ., New York, NY (United States); Sungkyunkwan Univ., Suwon (Korea)

To thoroughly elucidate how molecular conformation and photophysical properties of conjugated polymers (CPs) are related requires simultaneous probing of both. Here, previous efforts used fluorescence imaging with one nanometer accuracy (FIONA) to image CPs, which allowed simultaneous estimation of molecular conformation and probing of fluorescence intensity decay. We show that calculating the molecular radius of gyration for putative folded and unfolded poly(2-methoxy-5-(2'-ethylhexyloxy)1,4-phenylenevinylene) (MEH-PPV) molecules using FIONA underestimates molecular extension by averaging over emitters during localization. In contrast, employing algorithms based on single molecule high resolution imaging with photobleaching (SHRImP), including an approach we term all-frames SHRImP, allows localization of individual emitters. SHRImP processing corroborates that compact MEH-PPV molecules have distinct photophysical properties from extended ones. Estimated radii of gyration for isolated 168 kDa MEH-PPV molecules immobilized in polystyrene and exhibiting either stepwise or continuous intensity decays are found to be 12.6 and 25.3 nm, respectively, while the distance between exciton recombination sites is estimated to be ~10 nm independent of molecular conformation.

View Accepted Manuscript (DOE)

Research Organization:: Energy Frontier Research Centers (EFRC) (United States). Re-Defining Photovoltaic Efficiency Through Molecule Scale Control (RPEMSC)

Sponsoring Organization:: National Science Foundation; USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: SC0001085

OSTI ID:: 1370377

Journal Information:: ACS Nano, Journal Name: ACS Nano Journal Issue: 3 Vol. 9; ISSN 1936-0851

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
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Localizing Exciton Recombination Sites in Conformationally Distinct Single Conjugated Polymers by Super-resolution Fluorescence Imaging

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Cited By (2)

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