Peptide recognition by heterochromatin protein 1 (HP1) chromoshadow domains revisited: Plasticity in the pseudosymmetric histone binding site of human HP1

Liu, Yanli; Qin, Su; Lei, Ming; Tempel, Wolfram; Zhang, Yuzhe; Loppnau, Peter; Li, Yanjun; Min, Jinrong

doi:10.1074/jbc.M116.768374

Peptide recognition by heterochromatin protein 1 (HP1) chromoshadow domains revisited: Plasticity in the pseudosymmetric histone binding site of human HP1

Journal Article · Tue Feb 21 00:00:00 EST 2017 · Journal of Biological Chemistry

DOI:https://doi.org/10.1074/jbc.M116.768374· OSTI ID:1368285

Liu, Yanli ^[1]; Qin, Su ^[1]; Lei, Ming ^[1]; Tempel, Wolfram ^[1]; Zhang, Yuzhe ^[1]; Loppnau, Peter ^[1]; Li, Yanjun ^[1]; Min, Jinrong ^[1]

Univ. of Toronto, ON (Canada)

Heterochromatin protein 1 (HP1), a highly conserved non-histone chromosomal protein in eukaryotes, plays important roles in the regulation of gene transcription. Each of the three human homologs of HP1 includes a chromoshadow domain (CSD). The CSD interacts with various proteins bearing the PXVXL motif but also with a region of histone H3 that bears the similar PXXVXL motif. The latter interaction has not yet been resolved in atomic detail. Here we demonstrate that the CSDs of all three human HP1 homologs have comparable affinities to the PXXVXL motif of histone H3. The HP1 C-terminal extension enhances the affinity, as does the increasing length of the H3 peptide. The crystal structure of the human HP1γ CSD (CSDγ) in complex with an H3 peptide suggests that recognition of H3 by CSDγ to some extent resembles CSD-PXVXL interaction. Nevertheless, the prolyl residue of the PXXVXL motif appears to play a role distinct from that of Pro in the known HP1β CSD-PXVXL complexes. Lastly, we consequently generalize the historical CSD-PXVXL interaction model and expand the search scope for additional CSD binding partners.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: AbbVie, Boehringer Ingelheim; Canadian Inst. of Health Research (CIHR); National Natural Science Foundation of China; Ontario Genomics Inst.; Ontario Ministry of Economic Development and Innovation; USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); Wellcome Trust

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1368285

Journal Information:: Journal of Biological Chemistry, Journal Name: Journal of Biological Chemistry Journal Issue: 14 Vol. 292; ISSN 0021-9258

Publisher:: American Society for Biochemistry and Molecular BiologyCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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59 BASIC BIOLOGICAL SCIENCES
chromatin regulation
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heterochromatin
histone
peptide interaction

Peptide recognition by heterochromatin protein 1 (HP1) chromoshadow domains revisited: Plasticity in the pseudosymmetric histone binding site of human HP1

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