The ethanol pathway from Thermoanaerobacterium saccharolyticum improves ethanol production in Clostridium thermocellum

Hon, Shuen; Olson, Daniel G.; Holwerda, Evert K.; Lanahan, Anthony A.; Murphy, Sean J. L.; Maloney, Marybeth I.; Zheng, Tianyong; Papanek, Beth; Guss, Adam M.; Lynd, Lee R.

doi:10.1016/j.ymben.2017.06.011

The ethanol pathway from Thermoanaerobacterium saccharolyticum improves ethanol production in Clostridium thermocellum

Journal Article · Tue Jun 27 04:00:00 EDT 2017 · Metabolic Engineering

DOI:https://doi.org/10.1016/j.ymben.2017.06.011· OSTI ID:1367525

^[1]; Olson, Daniel G. ^[2]; Holwerda, Evert K. ^[2]; Lanahan, Anthony A. ^[2]; Murphy, Sean J. L. ^[2]; Maloney, Marybeth I. ^[2]; Zheng, Tianyong ^[2]; Papanek, Beth ^[3]; Guss, Adam M. ^[3]; Lynd, Lee R. ^[2]

Dartmouth College, Hanover, NH (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Dartmouth College
Dartmouth College, Hanover, NH (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Clostridium thermocellum ferments cellulose, is a promising candidate for ethanol production from cellulosic biomass, and has been the focus of studies aimed at improving ethanol yield. Thermoanaerobacterium saccharolyticum ferments hemicellulose, but not cellulose, and has been engineered to produce ethanol at high yield and titer. Recent research has led to the identification of four genes in T. saccharolyticum involved in ethanol production: adhE, nfnA, nfnB and adhA. We introduced these genes into C. thermocellum and observed significant improvements to ethanol yield, titer, and productivity. The four genes alone, however, were insufficient to achieve in C. thermocellum the ethanol yields and titers observed in engineered T. saccharolyticum strains, even when combined with gene deletions targeting hydrogen production. Here, this suggests that other parts of T. saccharolyticum metabolism may also be necessary to reproduce the high ethanol yield and titer phenotype in C. thermocellum.

View Accepted Manuscript (DOE)

Research Organization:: Dartmouth College, Hanover, NH (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1367525

Alternate ID(s):: OSTI ID: 1550373
OSTI ID: 1394382

Journal Information:: Metabolic Engineering, Journal Name: Metabolic Engineering Journal Issue: C Vol. 42; ISSN 1096-7176

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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The ethanol pathway from Thermoanaerobacterium saccharolyticum improves ethanol production in Clostridium thermocellum

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