In situ nuclear magnetic resonance microimaging of live biofilms in a microchannel

Renslow, R. S.; Marshall, M. J.; Tucker, A. E.; Chrisler, W. B.; Yu, X. -Y.

doi:10.1039/C7AN00078B

In situ nuclear magnetic resonance microimaging of live biofilms in a microchannel

Journal Article · Sat Dec 31 23:00:00 EST 2016 · Analyst

DOI:https://doi.org/10.1039/C7AN00078B· OSTI ID:1367386

Renslow, R. S.; Marshall, M. J.; Tucker, A. E.; Chrisler, W. B.;

Nuclear magnetic resonance (NMR) microimaging and spectroscopy was used to interrogate fluids of biological importance (e.g., water, buffer, medium solution) and live biofilms in a microchannel compatible for analyses at ambient pressure and under vacuum. Studies using buffer, growth medium, and actively growing Shewanella oneidensis biofilms were used to demonstrate in situ NMR microimaging measurement capabilities including velocity mapping, diffusion coefficient mapping, relaxometry, localized spectroscopy, and 2D and 3D imaging within a microchannel suitable for different analytical platforms. This technique is promising for diverse applications of correlative imaging using a portable microfluidic platform.

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1367386

Report Number(s):: PNNL-SA-109594; 48569; 49694

Journal Information:: Analyst, Journal Name: Analyst Journal Issue: 13 Vol. 142; ISSN 0003-2654

Publisher:: Royal Society of Chemistry

Country of Publication:: United States

Language:: English

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Related Subjects

Environmental Molecular Sciences Laboratory
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nuclear magnetic resonance
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In situ nuclear magnetic resonance microimaging of live biofilms in a microchannel

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