In situ nuclear magnetic resonance microimaging of live biofilms in a microchannel

Renslow, R. S.; Marshall, M. J.; Tucker, A. E.; Chrisler, W. B.; Yu, X. -Y.

doi:10.1039/C7AN00078B

Title: In situ nuclear magnetic resonance microimaging of live biofilms in a microchannel

Journal Article · Sun Jan 01 00:00:00 EST 2017 · Analyst

DOI:https://doi.org/10.1039/C7AN00078B· OSTI ID:1367386

Renslow, R. S.; Marshall, M. J.; Tucker, A. E.; Chrisler, W. B.;

Nuclear magnetic resonance (NMR) microimaging and spectroscopy was used to interrogate fluids of biological importance (e.g., water, buffer, medium solution) and live biofilms in a microchannel compatible for analyses at ambient pressure and under vacuum. Studies using buffer, growth medium, and actively growing Shewanella oneidensis biofilms were used to demonstrate in situ NMR microimaging measurement capabilities including velocity mapping, diffusion coefficient mapping, relaxometry, localized spectroscopy, and 2D and 3D imaging within a microchannel suitable for different analytical platforms. This technique is promising for diverse applications of correlative imaging using a portable microfluidic platform.

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Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1367386

Report Number(s):: PNNL-SA-109594; 48569; 49694

Journal Information:: Analyst, Vol. 142, Issue 13; ISSN 0003-2654

Publisher:: Royal Society of Chemistry

Country of Publication:: United States

Language:: English

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SALVI
microfluidics
diffusion coefficient
relaxometry
MRI
nuclear magnetic resonance
biofilm
Shewanella
Environmental Molecular Sciences Laboratory

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