Tracking microbial interactions with NanoSIMS

Musat, Niculina; Musat, Florin; Weber, Peter Kilian; Pett-Ridge, Jennifer

doi:10.1016/j.copbio.2016.06.007

Title: Tracking microbial interactions with NanoSIMS

Journal Article · Tue Jul 12 00:00:00 EDT 2016 · Current Opinion in Biotechnology

DOI:https://doi.org/10.1016/j.copbio.2016.06.007· OSTI ID:1366963

Musat, Niculina ^[1]; Musat, Florin ^[1]; Weber, Peter Kilian ^[2]; Pett-Ridge, Jennifer ^[2]

Helmholtz Centre for Environmental Research - UFZ, Leipzig (Germany)
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

The combination of stable isotope probing (SIP), NanoSIMS imaging and microbe identification via fluorescence in situ hybridization (FISH) is often used to link identity to function at the cellular level in microbial communities. Many opportunities remain for nanoSIP to identify metabolic interactions and nutrient fluxes within syntrophic associations and obligate symbioses where exchanges can be extremely rapid. However, additional data, such as genomic potential, gene expression or other imaging modalities are often critical to deciphering the mechanisms underlying specific interactions, and researchers must keep sample preparation artefacts in mind. Here we focus on recent applications of nanoSIP, particularly where used to track exchanges of isotopically labelled molecules between organisms. Here, we highlight metabolic interactions within syntrophic consortia, carbon/nitrogen fluxes between phototrophs and their heterotrophic partners, and symbiont–host nutrient sharing.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC52-07NA27344; SCW1039

OSTI ID:: 1366963

Alternate ID(s):: OSTI ID: 1434211

Report Number(s):: LLNL-JRNL-700939

Journal Information:: Current Opinion in Biotechnology, Vol. 41, Issue C; ISSN 0958-1669

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 72 works

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