A Complex Endomembrane System in the Archaeon Ignicoccus hospitalis Tapped by Nanoarchaeum equitans

Heimerl, Thomas; Flechsler, Jennifer; Pickl, Carolin; Heinz, Veronika; Salecker, Benjamin; Zweck, Josef; Wanner, Gerhard; Geimer, Stefan; Samson, Rachel Y.; Bell, Stephen D.; Huber, Harald; Wirth, Reinhard; Wurch, Louie; Podar, Mircea; Rachel, Reinhard

doi:10.3389/fmicb.2017.01072

A Complex Endomembrane System in the Archaeon Ignicoccus hospitalis Tapped by Nanoarchaeum equitans

Journal Article · Tue Jun 13 00:00:00 EDT 2017 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2017.01072· OSTI ID:1364327

Heimerl, Thomas ^[1]; Flechsler, Jennifer ^[2]; Pickl, Carolin ^[2]; Heinz, Veronika ^[3]; Salecker, Benjamin ^[3]; Zweck, Josef ^[4]; Wanner, Gerhard ^[2]; Geimer, Stefan ^[5]; Samson, Rachel Y. ^[6]; Bell, Stephen D. ^[6]; Huber, Harald ^[7]; Wirth, Reinhard ^[7]; Wurch, Louie ^[8]; Podar, Mircea ^[8]; Rachel, Reinhard ^[3]

Philipps Univ. of Marburg (Germany). LOEWE Research Center for Synthetic Microbiology (SYNMIKRO)
Ludwig Maximilian Univ., Munich (Germany). Biocenter LMU. Plant Development and Electron Microscopy
Univ. of Regensburg (Germany). Center for Electron Microscopy
Univ. of Regensburg (Germany). Inst. of Experimental and Applied Physics
Univ. of Bayreuth (Germany). Cell Biology and Electron Microscopy
Indiana Univ., Bloomington, IN (United States). Molecular and Cellular Biochemistry Dept.
Univ. of Regensburg (Germany). Microbiology Dept.
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division; Univ. of Tennessee, Knoxville, TN (United States). Microbiology Dept.

Based on serial sectioning, focused ion beam scanning electron microscopy (FIB/SEM), and electron tomography, we depict in detail the highly unusual anatomy of the marine hyperthermophilic crenarchaeon, Ignicoccus hospitalis. Our data support a complex and dynamic endomembrane system consisting of cytoplasmic protrusions, and with secretory function. Moreover, we reveal that the cytoplasm of the putative archaeal ectoparasite Nanoarchaeum equitans can get in direct contact with this endomembrane system, complementing and explaining recent proteomic, transcriptomic and metabolomic data on this inter-archaeal relationship. In addition, we identified a matrix of filamentous structures and/or tethers in the voluminous inter-membrane compartment (IMC) of I. hospitalis, which might be responsible for membrane dynamics. Overall, this unusual cellular compartmentalization, ultrastructure and dynamics in an archaeon that belongs to the recently proposed TACK superphylum prompts speculation that the eukaryotic endomembrane system might originate from Archaea.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Philipps Univ. of Marburg (Germany); Univ. of Regensburg (Germany); Univ. of Tennessee, Knoxville, TN (United States)

Sponsoring Organization:: German Research Foundation (DFG) (Germany); National Science Foundation (NSF) (United States); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); Wellcome Trust (United Kingdom)

Contributing Organization:: Indiana Univ., Bloomington, IN (United States); Ludwig Maximilian Univ., Munich (Germany); Univ. of Bayreuth (Germany)

Grant/Contract Number:: SC0006654

OSTI ID:: 1364327

Journal Information:: Frontiers in Microbiology, Journal Name: Frontiers in Microbiology Vol. 8; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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