RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production

Bewg, William P.; Poovaiah, Charleson; Lan, Wu; Ralph, John; Coleman, Heather D.

doi:10.1186/s13068-016-0683-y

Title: RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production

Journal Article · Tue Dec 20 00:00:00 EST 2016 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-016-0683-y· OSTI ID:1618659

Bewg, William P.;

;

Sugarcane is a subtropical crop that produces large amounts of biomass annually. It is a key agricultural crop in many countries for the production of sugar and other products. Residual bagasse following sucrose extraction is currently underutilized and it has potential as a carbohydrate source for the production of biofuels. As with all lignocellulosic crops, lignin acts as a barrier to accessing the polysaccharides, and as such, is the focus of transgenic efforts. In this study, we used RNAi to individually reduce the expression of three key genes in the lignin biosynthetic pathway in sugarcane. Furthermore, these genes, caffeoyl-CoA O-methyltransferase (CCoAOMT), ferulate 5-hydroxylase (F5H) and caffeic acid O-methyltransferase (COMT), impact lignin content and/or composition.

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Research Organization:: Syracuse Univ., NY (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: FC02-07ER64494

OSTI ID:: 1618659

Alternate ID(s):: OSTI ID: 1363952

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Vol. 9 Journal Issue: 1; ISSN 1754-6834

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: Netherlands

Language:: English

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Cited by: 24 works

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Related Subjects

09 BIOMASS FUELS
lignin biosynthesis
ferulate 5-hydroxylase
caffeic acid O-methyltransferase
caffeoyl-CoA O-methyltransferase
sugarcane
RNAi

RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production [Supplemental Data] Bewg, William; Poovaiah, Charleson; Lan, Wu https://doi.org/10.6084/m9.figshare.c.3649808 The current record is supplemented by this dataset	dataset	December 2016
RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production [Supplemental Data] Bewg, William; Poovaiah, Charleson; Lan, Wu https://doi.org/10.6084/m9.figshare.c.3649808.v1 The current record is supplemented by this dataset	dataset	December 2016
MOESM2 of RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production Bewg, William; Poovaiah, Charleson; Lan, Wu https://doi.org/10.6084/m9.figshare.c.3649808_d1 The current record is supplemented by this dataset	dataset	December 2016
MOESM1 of RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production Bewg, William; Lan, Wu; Ralph, John https://doi.org/10.6084/m9.figshare.c.3649808_d2 The current record is supplemented by this figure	figure	December 2016
MOESM5 of RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production Beng, William; Poovaiah, Charleson; Lan, Wu https://doi.org/10.6084/m9.figshare.c.3649808_d3 The current record is supplemented by this dataset	dataset	December 2016
MOESM5 of RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production Bewg, William; Poovaiah, Charleson; Lan, Wu https://doi.org/10.6084/m9.figshare.c.3649808_d3.v1 The current record is supplemented by this dataset	dataset	December 2016
MOESM3 of RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production Bewg, William; Lan, Wu; Ralph, John https://doi.org/10.6084/m9.figshare.c.3649808_d4 The current record is supplemented by this figure	figure	December 2016
MOESM4 of RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production Bewg, William; Poovaiah, Charleson; Lan, Wu https://doi.org/10.6084/m9.figshare.c.3649808_d5 The current record is supplemented by this figure	figure	December 2016
MOESM2 of RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production Bewg, William; Poovaiah, Charleson; Lan, Wu https://doi.org/10.6084/m9.figshare.c.3649808_d1.v1 The current record is supplemented by this dataset	dataset	December 2016
MOESM1 of RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production Bewg, William; Poovaiah, Charleson; Lan, Wu https://doi.org/10.6084/m9.figshare.c.3649808_d2.v1 The current record is supplemented by this dataset	dataset	December 2016
MOESM4 of RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production Bewg, William; Poovaiah, Charleson; Lan, Wu https://doi.org/10.6084/m9.figshare.c.3649808_d5.v1 The current record is supplemented by this figure	figure	December 2016
MOESM3 of RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production Bewg, William; Poovaiah, Charleson; Lan, Wu https://doi.org/10.6084/m9.figshare.c.3649808_d4.v1 The current record is supplemented by this dataset	dataset	December 2016