Simultaneous electrical recording of cardiac electrophysiology and contraction on chip

Qian, Fang; Huang, Chao; Lin, Yi-Dong; Ivanovskaya, Anna N.; O'Hara, Thomas J.; Booth, Ross H.; Creek, Cameron J.; Enright, Heather A.; Soscia, David A.; Belle, Anna M.; Liao, Ronglih; Lightstone, Felice C.; Kulp, Kristen S.; Wheeler, Elizabeth K.

doi:10.1039/C7LC00210F

Simultaneous electrical recording of cardiac electrophysiology and contraction on chip

Journal Article · Tue Apr 18 00:00:00 EDT 2017 · Lab on a Chip

DOI:https://doi.org/10.1039/C7LC00210F· OSTI ID:1361595

^[1]; Huang, Chao ^[1]; Lin, Yi-Dong ^[2]; Ivanovskaya, Anna N. ^[1]; O'Hara, Thomas J. ^[1]; Booth, Ross H. ^[1]; Creek, Cameron J. ^[1]; Enright, Heather A. ^[1]; Soscia, David A. ^[1]; Belle, Anna M. ^[1]; Liao, Ronglih ^[2]; Lightstone, Felice C. ^[1]; Kulp, Kristen S. ^[1]; Wheeler, Elizabeth K. ^[1]

Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Brigham and Women's Hospital (Harvard Medical School), Boston, MA (United States). Dept. of Medicine

Prevailing commercialized cardiac platforms for in vitro drug development utilize planar microelectrode arrays to map action potentials, or impedance sensing to record contraction in real time, but cannot record both functions on the same chip with high spatial resolution. We report a novel cardiac platform that can record cardiac tissue adhesion, electrophysiology, and contractility on the same chip. The platform integrates two independent yet interpenetrating sensor arrays: a microelectrode array for field potential readouts and an interdigitated electrode array for impedance readouts. Together, these arrays provide real-time, non-invasive data acquisition of both cardiac electrophysiology and contractility under physiological conditions and under drug stimuli. Furthermore, we cultured human induced pluripotent stem cell-derived cardiomyocytes as a model system, and used to validate the platform with an excitation–contraction decoupling chemical. Preliminary data using the platform to investigate the effect of the drug norepinephrine are combined with computational efforts. Finally, this platform provides a quantitative and predictive assay system that can potentially be used for comprehensive assessment of cardiac toxicity earlier in the drug discovery process.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1361595

Report Number(s):: LLNL-JRNL--714498

Journal Information:: Lab on a Chip, Journal Name: Lab on a Chip Journal Issue: 10 Vol. 17; ISSN LCAHAM; ISSN 1473-0197

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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