The structure of Zika virus NS5 reveals a conserved domain conformation

Wang, Boxiao; Tan, Xiao -Feng; Thurmond, Stephanie; Zhang, Zhi -Min; Lin, Asher; Hai, Rong; Song, Jikui

doi:10.1038/ncomms14763

The structure of Zika virus NS5 reveals a conserved domain conformation

Journal Article · Mon Mar 27 00:00:00 EDT 2017 · Nature Communications

DOI:https://doi.org/10.1038/ncomms14763· OSTI ID:1361212

Wang, Boxiao ^[1]; Tan, Xiao -Feng ^[2]; Thurmond, Stephanie ^[2]; Zhang, Zhi -Min ^[2]; Lin, Asher ^[2]; Hai, Rong ^[2]; Song, Jikui ^[2]

Univ. of California, Riverside, CA (United States); Department of Biochemistry, University of California, Riverside, Riverside, California 92521, USA
Univ. of California, Riverside, CA (United States)

The recent outbreak of Zika virus (ZIKV) has imposed a serious threat to public health. Here we report the crystal structure of the ZIKV NS5 protein in complex with S-adenosyl-L-homocysteine, in which the tandem methyltransferase (MTase) and RNA-dependent RNA polymerase (RdRp) domains stack into one of the two alternative conformations of flavivirus NS5 proteins. In conclusion, the activity of this NS5 protein is verified through a de novo RdRp assay on a subgenomic ZIKV RNA template. Importantly, our structural analysis leads to the identification of a potential drug-binding site of ZIKV NS5, which might facilitate the development of novel antivirals for ZIKV.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of California, Riverside, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1361212

Journal Information:: Nature Communications, Journal Name: Nature Communications Vol. 8; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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