Map-based cloning of a gene controlling Omega-3 fatty acid desaturation in Arabidopsis
- Michigan State Univ., East Lansing, MI (United States); and others
A gene from the flowering plant Arabidopsis thaliana that encodes an omega-3 desaturase was cloned on the basis of the genetic map position of a mutation affecting membrane and storage lipid fatty acid composition. Yeast artificial chromosomes covering the genetic locus were identified and used to probe a seed complementary DNA library. A complementary DNA clone for the desaturase was identified and introduced into roots of both wild-type and mutant plants by Ti plasmid-mediated transformation. Transgenic tissues of both mutant and wild-type plants had significantly increased amounts of the fatty acid produced by this desaturase. 24 refs., 2 figs., 1 tabs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 135890
- Journal Information:
- Science, Journal Name: Science Journal Issue: 5086 Vol. 258; ISSN SCIEAS; ISSN 0036-8075
- Country of Publication:
- United States
- Language:
- English
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