Genomic comparisons of a bacterial lineage that inhabits both marine and terrestrial deep subsurface systems

Jungbluth, Sean P.; Glavina del Rio, Tijana; Tringe, Susannah G.; Stepanauskas, Ramunas; Rappé, Michael S.

doi:10.7717/peerj.3134

Title: Genomic comparisons of a bacterial lineage that inhabits both marine and terrestrial deep subsurface systems

Journal Article · Thu Apr 06 00:00:00 EDT 2017 · PeerJ

DOI:https://doi.org/10.7717/peerj.3134· OSTI ID:1358388

Jungbluth, Sean P. ^[1]; Glavina del Rio, Tijana ^[2]; Tringe, Susannah G. ^[2]; Stepanauskas, Ramunas ^[3]; Rappé, Michael S. ^[4]

Univ. of Hawaii at Manoa, Honolulu, HI (United States); Univ. of Southern California, Los Angeles, CA (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Bigelow Lab. for Ocean Sciences, East Boothbay, ME (United States)
Univ. of Hawaii at Manoa, Kaneohe, HI (United States)

It is generally accepted that diverse, poorly characterized microorganisms reside deep within Earth’s crust. One such lineage of deep subsurface-dwelling bacteria is an uncultivated member of theFirmicutesphylum that can dominate molecular surveys from both marine and continental rock fracture fluids, sometimes forming the sole member of a single-species microbiome. Here, we reconstructed a genome from basalt-hosted fluids of the deep subseafloor along the eastern Juan de Fuca Ridge flank and used a phylogenomic analysis to show that, despite vast differences in geographic origin and habitat, it forms a monophyletic clade with the terrestrial deep subsurface genome of “CandidatusDesulforudis audaxviator” MP104C. While a limited number of differences were observed between the marine genome of “CandidatusDesulfopertinax cowenii” modA32 and its terrestrial relative that may be of potential adaptive importance, here it is revealed that the two are remarkably similar thermophiles possessing the genetic capacity for motility, sporulation, hydrogenotrophy, chemoorganotrophy, dissimilatory sulfate reduction, and the ability to fix inorganic carbon via the Wood-Ljungdahl pathway for chemoautotrophic growth. Our results provide insights into the genetic repertoire within marine and terrestrial members of a bacterial lineage that is widespread in the global deep subsurface biosphere, and provides a natural means to investigate adaptations specific to these two environments.

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Research Organization:: USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1358388

Alternate ID(s):: OSTI ID: 1379633

Journal Information:: PeerJ, Vol. 5; ISSN 2167-8359

Publisher:: PeerJ Inc.Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 23 works

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59 BASIC BIOLOGICAL SCIENCES
deep subsurface
microorganisms
Firmicutes
Juan de Fuca Ridge
chemoautotrophy
basement biosphere
sulfate reduction
sporulation
genomic
metagenomic

Title: Genomic comparisons of a bacterial lineage that inhabits both marine and terrestrial deep subsurface systems

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