Dual interaction of the Hsp70 J-protein cochaperone Zuotin with the 40S and 60S ribosomal subunits

Lee, Kanghyun; Sharma, Ruchika; Shrestha, Om Kumar; Bingman, Craig A.; Craig, Elizabeth A.

doi:10.1038/nsmb.3299

Dual interaction of the Hsp70 J-protein cochaperone Zuotin with the 40S and 60S ribosomal subunits

Journal Article · Mon Sep 26 00:00:00 EDT 2016 · Nature Structural & Molecular Biology

DOI:https://doi.org/10.1038/nsmb.3299· OSTI ID:1357642

Lee, Kanghyun ^[1]; Sharma, Ruchika ^[1]; Shrestha, Om Kumar ^[1]; Bingman, Craig A. ^[1]; Craig, Elizabeth A. ^[1]

Univ. of Wisconsin, Madison, WI (United States)

Ribosome-associated J protein–Hsp70 chaperones promote nascent-polypeptide folding and normal translational fidelity. The J protein Zuo1 is known to span the ribosomal subunits, but understanding of its function is limited. Here, in this paper, we present new structural and cross-linking data allowing more precise positioning of Saccharomyces cerevisiae Zuo1 near the 60S polypeptide-exit site and suggesting interactions of Zuo1 with the ribosomal protein eL31 and 25S rRNA helix 24. The junction between the 60S-interacting and subunit-spanning helices is a hinge that positions Zuo1 on the 40S yet accommodates subunit rotation. Interaction between the Zuo1 C terminus and 40S occurs via 18S rRNA expansion segment 12 (ES12) of helix 44, which originates at the decoding site. Deletions in either ES12 or the Zuo1 C terminus alter readthrough of stop codons and –1 frameshifting. Our study offers insight into how this cotranslational chaperone system may monitor decoding-site activity and nascent-polypeptide transit, thereby coordinating protein translation and folding.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: National Cancer Institute (NCI); National Institute of General Medical Sciences (NIGMS); National Institutes of Health (NIH); USDOE Office of Science (SC)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1357642

Journal Information:: Nature Structural & Molecular Biology, Journal Name: Nature Structural & Molecular Biology Journal Issue: 11 Vol. 23; ISSN 1545-9993

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

References (83)

Comparative Protein Structure Modeling Using MODELLER Webb, Benjamin; Sali, Andrej Current Protocols in Bioinformatics, Vol. 47, Issue 1 https://doi.org/10.1002/0471250953.bi0506s47	journal	September 2014
Comparative Protein Structure Modeling Using MODELLER Webb, Benjamin; Sali, Andrej Current Protocols in Protein Science, Vol. 86, Issue 1 https://doi.org/10.1002/cpps.20	journal	November 2016
UCSF Chimera?A visualization system for exploratory research and analysis Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C. Journal of Computational Chemistry, Vol. 25, Issue 13 https://doi.org/10.1002/jcc.20084	journal	January 2004
Four major transcriptional responses in the methionine/threonine biosynthetic pathway ofSaccharomyces cerevisiae Mountain, Harry A.; Byström, Anders S.; Larsen, Jörgen Tang Yeast, Vol. 7, Issue 8 https://doi.org/10.1002/yea.320070804	journal	November 1991
Exploring the gas access routes in a [NiFeSe] hydrogenase using crystals pressurized with krypton and oxygen Zacarias, Sónia; Temporão, Adriana; Carpentier, Philippe JBIC Journal of Biological Inorganic Chemistry, Vol. 25, Issue 6 https://doi.org/10.1007/s00775-020-01814-y	journal	August 2020
The translation machinery and 70 kd heat shock protein cooperate in protein synthesis Nelson, R. John; Ziegelhoffer, Thomas; Nicolet, Charles Cell, Vol. 71, Issue 1 https://doi.org/10.1016/0092-8674(92)90269-I	journal	October 1992
Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds Mumberg, Dominik; Müller, Rolf; Funk, Martin Gene, Vol. 156, Issue 1 https://doi.org/10.1016/0378-1119(95)00037-7	journal	April 1995
The Ribosomal Exit Tunnel Functions as a Discriminating Gate Nakatogawa, Hitoshi; Ito, Koreaki Cell, Vol. 108, Issue 5 https://doi.org/10.1016/S0092-8674(02)00649-9	journal	March 2002
A conserved domain important for association of eukaryotic J-protein co-chaperones Jjj1 and Zuo1 with the ribosome Kaschner, Lindsey A.; Sharma, Ruchika; Shrestha, Om Kumar Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol. 1853, Issue 5 https://doi.org/10.1016/j.bbamcr.2015.01.014	journal	May 2015
Dynamics of co-translational protein targeting Elvekrog, Margaret M.; Walter, Peter Current Opinion in Chemical Biology, Vol. 29 https://doi.org/10.1016/j.cbpa.2015.09.016	journal	December 2015
Fidelity at the Molecular Level: Lessons from Protein Synthesis Zaher, Hani S.; Green, Rachel Cell, Vol. 136, Issue 4 https://doi.org/10.1016/j.cell.2009.01.036	journal	February 2009
The Cotranslational Function of Ribosome-Associated Hsp70 in Eukaryotic Protein Homeostasis Willmund, Felix; del Alamo, Marta; Pechmann, Sebastian Cell, Vol. 152, Issue 1-2 https://doi.org/10.1016/j.cell.2012.12.001	journal	January 2013
Ribosome kinetics and aa-tRNA competition determine rate and fidelity of peptide synthesis Fluitt, Aaron; Pienaar, Elsje; Viljoen, Hendrik Computational Biology and Chemistry, Vol. 31, Issue 5-6 https://doi.org/10.1016/j.compbiolchem.2007.07.003	journal	October 2007
Discrimination Between Defects in Elongation Fidelity and Termination Efficiency Provides Mechanistic Insights into Translational Readthrough Salas-Marco, Joe; Bedwell, David M. Journal of Molecular Biology, Vol. 348, Issue 4 https://doi.org/10.1016/j.jmb.2005.03.025	journal	May 2005
Statics of the Ribosomal Exit Tunnel: Implications for Cotranslational Peptide Folding, Elongation Regulation, and Antibiotics Binding Fulle, Simone; Gohlke, Holger Journal of Molecular Biology, Vol. 387, Issue 2 https://doi.org/10.1016/j.jmb.2009.01.037	journal	March 2009
Unfolding of the C-Terminal Domain of the J-Protein Zuo1 Releases Autoinhibition and Activates Pdr1-Dependent Transcription Ducett, Jeanette K.; Peterson, Francis C.; Hoover, Lindsey A. Journal of Molecular Biology, Vol. 425, Issue 1 https://doi.org/10.1016/j.jmb.2012.09.020	journal	January 2013
How Hsp70 Molecular Machines Interact with Their Substrates to Mediate Diverse Physiological Functions Clerico, Eugenia M.; Tilitsky, Joseph M.; Meng, Wenli Journal of Molecular Biology, Vol. 427, Issue 7 https://doi.org/10.1016/j.jmb.2015.02.004	journal	April 2015
The Ribosome as a Hub for Protein Quality Control Pechmann, Sebastian; Willmund, Felix; Frydman, Judith Molecular Cell, Vol. 49, Issue 3 https://doi.org/10.1016/j.molcel.2013.01.020	journal	February 2013
Ribosome Rescue and Protein Quality Control in Concert Hilal, Tarek; Spahn, Christian M. T. Molecular Cell, Vol. 57, Issue 3 https://doi.org/10.1016/j.molcel.2015.01.029	journal	February 2015
The ribosomal tunnel as a functional environment for nascent polypeptide folding and translational stalling Wilson, Daniel N.; Beckmann, Roland Current Opinion in Structural Biology, Vol. 21, Issue 2 https://doi.org/10.1016/j.sbi.2011.01.007	journal	April 2011
Co-translational mechanisms of protein maturation Gloge, Felix; Becker, Annemarie H.; Kramer, Günter Current Opinion in Structural Biology, Vol. 24 https://doi.org/10.1016/j.sbi.2013.11.004	journal	February 2014
Structures of Yeast 80S Ribosome-tRNA Complexes in the Rotated and Nonrotated Conformations Svidritskiy, Egor; Brilot, Axel F.; Koh, Cha San Structure, Vol. 22, Issue 8 https://doi.org/10.1016/j.str.2014.06.003	journal	August 2014
Ribosome-associated chaperones as key players in proteostasis Preissler, Steffen; Deuerling, Elke Trends in Biochemical Sciences, Vol. 37, Issue 7 https://doi.org/10.1016/j.tibs.2012.03.002	journal	July 2012
Less is more: improving proteostasis by translation slow down Sherman, Michael Y.; Qian, Shu-Bing Trends in Biochemical Sciences, Vol. 38, Issue 12 https://doi.org/10.1016/j.tibs.2013.09.003	journal	December 2013
Changed in translation: mRNA recoding by −1 programmed ribosomal frameshifting Caliskan, Neva; Peske, Frank; Rodnina, Marina V. Trends in Biochemical Sciences, Vol. 40, Issue 5 https://doi.org/10.1016/j.tibs.2015.03.006	journal	May 2015
Enhanced Bacterial Protein Expression During Auto-Induction Obtained by Alteration of Lac Repressor Dosage and Medium Composition Blommel, Paul G.; Becker, Katie J.; Duvnjak, Petar Biotechnology Progress, Vol. 23, Issue 3 https://doi.org/10.1021/bp070011x	journal	January 2007
Caught in the Act: Covalent Cross-Linking Captures Activator–Coactivator Interactions in Vivo Krishnamurthy, Malathy; Dugan, Amanda; Nwokoye, Adaora ACS Chemical Biology, Vol. 6, Issue 12 https://doi.org/10.1021/cb200308e	journal	November 2011
Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics Carter, Andrew P.; Clemons, William M.; Brodersen, Ditlev E. Nature, Vol. 407, Issue 6802 https://doi.org/10.1038/35030019	journal	September 2000
Fast, scalable generation of high‐quality protein multiple sequence alignments using Clustal Omega Sievers, Fabian; Wilm, Andreas; Dineen, David Molecular Systems Biology, Vol. 7, Issue 1 https://doi.org/10.1038/msb.2011.75	journal	January 2011
Transcriptional activation of polycomb-repressed genes by ZRF1 Richly, Holger; Rocha-Viegas, Luciana; Ribeiro, Joana Domingues Nature, Vol. 468, Issue 7327 https://doi.org/10.1038/nature09574	journal	December 2010
A cytosolic network suppressing mitochondria-mediated proteostatic stress and cell death Wang, Xiaowen; Chen, Xin Jie Nature, Vol. 524, Issue 7566 https://doi.org/10.1038/nature14859	journal	July 2015
Mistargeted mitochondrial proteins activate a proteostatic response in the cytosol Wrobel, Lidia; Topf, Ulrike; Bragoszewski, Piotr Nature, Vol. 524, Issue 7566 https://doi.org/10.1038/nature14951	journal	August 2015
I-TASSER: a unified platform for automated protein structure and function prediction Roy, Ambrish; Kucukural, Alper; Zhang, Yang Nature Protocols, Vol. 5, Issue 4 https://doi.org/10.1038/nprot.2010.5	journal	March 2010
The HSP70 chaperone machinery: J proteins as drivers of functional specificity Kampinga, Harm H.; Craig, Elizabeth A. Nature Reviews Molecular Cell Biology, Vol. 11, Issue 8 https://doi.org/10.1038/nrm2941	journal	August 2010
Cryo-EM structures of Arx1 and maturation factors Rei1 and Jjj1 bound to the 60S ribosomal subunit Greber, Basil J.; Boehringer, Daniel; Montellese, Christian Nature Structural & Molecular Biology, Vol. 19, Issue 12 https://doi.org/10.1038/nsmb.2425	journal	November 2012
Structural characterization of a eukaryotic chaperone—the ribosome-associated complex Leidig, Christoph; Bange, Gert; Kopp, Jürgen Nature Structural & Molecular Biology, Vol. 20, Issue 1 https://doi.org/10.1038/nsmb.2447	journal	December 2012
Structural basis for interaction of a cotranslational chaperone with the eukaryotic ribosome Zhang, Yixiao; Ma, Chengying; Yuan, Yi Nature Structural & Molecular Biology, Vol. 21, Issue 12 https://doi.org/10.1038/nsmb.2908	journal	November 2014
SRP meets the ribosome Wild, Klemens; Halic, Mario; Sinning, Irmgard Nature Structural & Molecular Biology, Vol. 11, Issue 11 https://doi.org/10.1038/nsmb853	journal	November 2004
The Hsp70 Ssz1 modulates the function of the ribosome-associated J-protein Zuo1 Huang, Peggy; Gautschi, Matthias; Walter, William Nature Structural & Molecular Biology, Vol. 12, Issue 6 https://doi.org/10.1038/nsmb942	journal	May 2005
Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation Tillu, Vikas A.; Rae, James; Gao, Ya Nature Communications, Vol. 12, Issue 1 https://doi.org/10.1038/s41467-021-21035-4	journal	February 2021
An SHR–SCR module specifies legume cortical cell fate to enable nodulation Dong, Wentao; Zhu, Yayun; Chang, Huizhong Nature, Vol. 589, Issue 7843 https://doi.org/10.1038/s41586-020-3016-z	journal	December 2020
The chaperones MPP11 and Hsp70L1 form the mammalian ribosome-associated complex Otto, H.; Conz, C.; Maier, P. Proceedings of the National Academy of Sciences, Vol. 102, Issue 29 https://doi.org/10.1073/pnas.0504400102	journal	July 2005
The in vivo function of the ribosome-associated Hsp70, Ssz1, does not require its putative peptide-binding domain Hundley, H.; Eisenman, H.; Walter, W. Proceedings of the National Academy of Sciences, Vol. 99, Issue 7 https://doi.org/10.1073/pnas.062048399	journal	April 2002
A functional chaperone triad on the yeast ribosome Gautschi, M.; Mun, A.; Ross, S. Proceedings of the National Academy of Sciences, Vol. 99, Issue 7 https://doi.org/10.1073/pnas.062048599	journal	April 2002
RAC, a stable ribosome-associated complex in yeast formed by the DnaK-DnaJ homologs Ssz1p and zuotin Gautschi, M.; Lilie, H.; Funfschilling, U. Proceedings of the National Academy of Sciences, Vol. 98, Issue 7 https://doi.org/10.1073/pnas.071057198	journal	March 2001
Role for the molecular chaperones Zuo1 and Ssz1 in quorum sensing via activation of the transcription factor Pdr1 Prunuske, A. J.; Waltner, J. K.; Kuhn, P. Proceedings of the National Academy of Sciences, Vol. 109, Issue 2 https://doi.org/10.1073/pnas.1119184109	journal	December 2011
Structural Analysis of the Ribosome-associated Complex (RAC) Reveals an Unusual Hsp70/Hsp40 Interaction Fiaux, Jocelyne; Horst, Janina; Scior, Annika Journal of Biological Chemistry, Vol. 285, Issue 5 https://doi.org/10.1074/jbc.M109.075804	journal	November 2009
Yeast 18 S rRNA Is Directly Involved in the Ribosomal Response to Stringent AUG Selection during Translation Initiation Nemoto, Naoki; Singh, Chingakham Ranjit; Udagawa, Tsuyoshi Journal of Biological Chemistry, Vol. 285, Issue 42 https://doi.org/10.1074/jbc.M110.146662	journal	August 2010
Interaction of Nascent Chains with the Ribosomal Tunnel Proteins Rpl4, Rpl17, and Rpl39 of Saccharomyces cerevisiae Zhang, Ying; Wölfle, Tina; Rospert, Sabine Journal of Biological Chemistry, Vol. 288, Issue 47 https://doi.org/10.1074/jbc.M113.508283	journal	September 2013
Architecture of the TIM23 Inner Mitochondrial Translocon and Interactions with the Matrix Import Motor Ting, See-Yeun; Schilke, Brenda A.; Hayashi, Masaya Journal of Biological Chemistry, Vol. 289, Issue 41 https://doi.org/10.1074/jbc.M114.588152	journal	August 2014
Functional Characterization of the Atypical Hsp70 Subunit of Yeast Ribosome-associated Complex Conz, C.; Otto, H.; Peisker, K. Journal of Biological Chemistry, Vol. 282, Issue 47 https://doi.org/10.1074/jbc.M706737200	journal	October 2007
A trans-membrane segment inside the ribosome exit tunnel triggers RAMP4 recruitment to the Sec61p translocase Pool, Martin R. The Journal of Cell Biology, Vol. 185, Issue 5 https://doi.org/10.1083/jcb.200807066	journal	May 2009
A ribosome-anchored chaperone network that facilitates eukaryotic ribosome biogenesis Albanèse, Véronique; Reissmann, Stefanie; Frydman, Judith Journal of Cell Biology, Vol. 189, Issue 1 https://doi.org/10.1083/jcb.201001054	journal	April 2010
Polytopic membrane protein folding at L17 in the ribosome tunnel initiates cyclical changes at the translocon Lin, Pen-Jen; Jongsma, Candice G.; Pool, Martin R. The Journal of Cell Biology, Vol. 195, Issue 1 https://doi.org/10.1083/jcb.201103118	journal	September 2011
ZRF1 mediates remodeling of E3 ligases at DNA lesion sites during nucleotide excision repair Gracheva, Ekaterina; Chitale, Shalaka; Wilhelm, Thomas Journal of Cell Biology, Vol. 213, Issue 2 https://doi.org/10.1083/jcb.201506099	journal	April 2016
Ribosome-associated Complex Binds to Ribosomes in Close Proximity of Rpl31 at the Exit of the Polypeptide Tunnel in Yeast Peisker, Kristin; Braun, Daniel; Wölfle, Tina Molecular Biology of the Cell, Vol. 19, Issue 12 https://doi.org/10.1091/mbc.E08-06-0661	journal	December 2008
Ribosome-associated Complex Binds to Ribosomes in Close Proximity of Rpl31 at the Exit of the Polypeptide Tunnel in Yeast Peisker, Kristin; Braun, Daniel; Wölfle, Tina Molecular Biology of the Cell, Vol. 19, Issue 12 https://doi.org/10.1091/mbc.e08-06-0661	journal	December 2008
Impact of the six nucleotides downstream of the stop codon on translation termination Namy, Olivier; Hatin, Isabelle; Rousset, Jean‐Pierre EMBO reports, Vol. 2, Issue 9 https://doi.org/10.1093/embo-reports/kve176	journal	September 2001
Zuotin, a ribosome-associated DnaJmolecular chaperone Yan, W. The EMBO Journal, Vol. 17, Issue 16 https://doi.org/10.1093/emboj/17.16.4809	journal	August 1998
A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Sikorski, R. S.; Hieter, P. Genetics, Vol. 122, Issue 1 https://doi.org/10.1093/genetics/122.1.19	journal	May 1989
MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets Kumar, Sudhir; Stecher, Glen; Tamura, Koichiro Molecular Biology and Evolution, Vol. 33, Issue 7 https://doi.org/10.1093/molbev/msw054	journal	March 2016
Complete deletion of yeast chromosomal rDNA repeats and integration of a new rDNA repeat: use of rDNA deletion strains for functional analysis of rDNA promoter elements in vivo Wai, H. H. Nucleic Acids Research, Vol. 28, Issue 18 https://doi.org/10.1093/nar/28.18.3524	journal	September 2000
Two Distinct Conformations of the Conserved RNA-rich Decoding Center of the Small Ribosomal Subunit Are Recognized by tRNAs and Release Factors Youngman, E. M.; Cochella, L.; Brunelle, J. L. Cold Spring Harbor Symposia on Quantitative Biology, Vol. 71, Issue 0 https://doi.org/10.1101/sqb.2006.71.036	journal	January 2006
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/S0907444904019158	journal	November 2004
MolProbity : all-atom structure validation for macromolecular crystallography Chen, Vincent B.; Arendall, W. Bryan; Headd, Jeffrey J. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 1 https://doi.org/10.1107/S0907444909042073	journal	December 2009
XDS Kabsch, Wolfgang Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2 https://doi.org/10.1107/S0907444909047337	journal	January 2010
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Activation of pleiotropic drug resistance by the J-protein and Hsp70-related proteins, Zuo1 and Ssz1: Ssz1 and Zuo1 function in pleiotropic drug resistance Eisenman, Helene C.; Craig, Elizabeth A. Molecular Microbiology, Vol. 53, Issue 1 https://doi.org/10.1111/j.1365-2958.2004.04134.x	journal	June 2004
Human Mpp11 J Protein: Ribosome-Tethered Molecular Chaperones Are Ubiquitous Hundley, H. A. Science, Vol. 308, Issue 5724 https://doi.org/10.1126/science.1109247	journal	May 2005
Rqc2p and 60 S ribosomal subunits mediate mRNA-independent elongation of nascent chains Shen, Peter S.; Park, Joseph; Qin, Yidan Science, Vol. 347, Issue 6217 https://doi.org/10.1126/science.1259724	journal	January 2015
Functional Specificity Among Hsp70 Molecular Chaperones James, P.; Pfund, C.; Craig, E. A. Science, Vol. 275, Issue 5298 https://doi.org/10.1126/science.275.5298.387	journal	January 1997
Specific Effects of Ribosome-Tethered Molecular Chaperones on Programmed −1 Ribosomal Frameshifting Muldoon-Jacobs, Kristi L.; Dinman, Jonathan D. Eukaryotic Cell, Vol. 5, Issue 4 https://doi.org/10.1128/EC.5.4.762-770.2006	journal	April 2006
The Chaperone Network Connected to Human Ribosome-Associated Complex Jaiswal, H.; Conz, C.; Otto, H. Molecular and Cellular Biology, Vol. 31, Issue 6 https://doi.org/10.1128/MCB.00986-10	journal	January 2011
Self-regulation of 70-kilodalton heat shock proteins in Saccharomyces cerevisiae. Stone, D. E.; Craig, E. A. Molecular and Cellular Biology, Vol. 10, Issue 4 https://doi.org/10.1128/MCB.10.4.1622	journal	April 1990
A heat shock transcription factor with reduced activity suppresses a yeast HSP70 mutant. Halladay, J. T.; Craig, E. A. Molecular and Cellular Biology, Vol. 15, Issue 9 https://doi.org/10.1128/MCB.15.9.4890	journal	September 1995
Ribosomal protein L32 of Saccharomyces cerevisiae influences both the splicing of its own transcript and the processing of rRNA. Vilardell, J.; Warner, J. R. Molecular and Cellular Biology, Vol. 17, Issue 4 https://doi.org/10.1128/MCB.17.4.1959	journal	April 1997
The Ribosome-Bound Chaperones RAC and Ssb1/2p Are Required for Accurate Translation in Saccharomyces cerevisiae Rakwalska, M.; Rospert, S. Molecular and Cellular Biology, Vol. 24, Issue 20 https://doi.org/10.1128/MCB.24.20.9186-9197.2004	journal	September 2004
Self-regulation of 70-kilodalton heat shock proteins in Saccharomyces cerevisiae Stone, D. E.; Craig, E. A. Molecular and Cellular Biology, Vol. 10, Issue 4 https://doi.org/10.1128/mcb.10.4.1622-1632.1990	journal	April 1990
Molecular Chaperone Functions in Protein Folding and Proteostasis Kim, Yujin E.; Hipp, Mark S.; Bracher, Andreas Annual Review of Biochemistry, Vol. 82, Issue 1 https://doi.org/10.1146/annurev-biochem-060208-092442	journal	June 2013
Structural Basis of the Unfolded Protein Response Korennykh, Alexei; Walter, Peter Annual Review of Cell and Developmental Biology, Vol. 28, Issue 1 https://doi.org/10.1146/annurev-cellbio-101011-155826	journal	November 2012
SRP meets the ribosome Wild, Klemens; Halic, Mario; Sinning, Irmgard GBM Annual Spring meeting Mosbach 2005, Vol. 2005, Issue Spring https://doi.org/10.1240/sav_gbm_2005_m_001095	journal	April 2005
A comprehensive analysis of translational missense errors in the yeast Saccharomyces cerevisiae Kramer, E. B.; Vallabhaneni, H.; Mayer, L. M. RNA, Vol. 16, Issue 9 https://doi.org/10.1261/rna.2201210	journal	July 2010
PHENIX: a comprehensive Python-based system for macromolecular structure solution. Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.45787	text	January 2010

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