Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli

Yung, Mimi C.; Bourguet, Feliza A.; Carpenter, Timothy S.; Coleman, Matthew A.

doi:10.1186/s12934-017-0685-x

Title: Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli

Journal Article · Wed Apr 26 00:00:00 EDT 2017 · Microbial Cell Factories

DOI:https://doi.org/10.1186/s12934-017-0685-x· OSTI ID:1618889

; Bourguet, Feliza A.; Carpenter, Timothy S.; Coleman, Matthew A.

Recombinant expression of toxic proteins remains a challenging problem. Furthermore, one potential method to shield toxicity and thus improve expression of these proteins is to encapsulate them within protein compartments to sequester them away from their targets. Many bacteria naturally produce so-called bacterial microcompartments (BMCs) in which enzymes comprising a biosynthetic pathway are encapsulated in a proteinaeous shell, which is in part thought to shield the cells from the toxicity of reaction intermediates. As a proof-of-concept, we attempted to encapsulate toxic, lysis protein E (E) from bacteriophage ΦX174 inside recombinant BMCs to enhance its expression and achieve higher yields during downstream purification.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: 15-LW-013; AC52-07NA27344

OSTI ID:: 1618889

Alternate ID(s):: OSTI ID: 1357376

Report Number(s):: LLNL-JRNL-725506; 71; PII: 685

Journal Information:: Microbial Cell Factories, Journal Name: Microbial Cell Factories Vol. 16 Journal Issue: 1; ISSN 1475-2859

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: United Kingdom

Language:: English

Citation Metrics:

Cited by: 16 works

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Cited By (3)

Bio-engineering of bacterial microcompartments: a mini review Planamente, Sara; Frank, Stefanie Biochemical Society Transactions, Vol. 47, Issue 3 https://doi.org/10.1042/bst20170564	journal	June 2019
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Engineering the Bacterial Microcompartment Domain for Molecular Scaffolding Applications Young, Eric J.; Burton, Rodney; Mahalik, Jyoti P. Frontiers in Microbiology, Vol. 8 https://doi.org/10.3389/fmicb.2017.01441	journal	July 2017

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
bacterial microcompartment
BMC
lysis protein E
bacteriophage phiX174
toxic protein expression

Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli [Supplemental Data] Yung, Mimi; Carpenter, Timothy; Coleman, Matthew https://doi.org/10.6084/m9.figshare.c.3757043 The current record is supplemented by this figure	figure	April 2017
Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli [Supplemental Data] Yung, Mimi; Bourguet, Feliza; Carpenter, Timothy https://doi.org/10.6084/m9.figshare.c.3757043.v1 The current record is supplemented by this dataset	dataset	April 2017
MOESM1 of Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ĎX174 in Escherichia coli Yung, Mimi; Bourguet, Feliza; Carpenter, Timothy https://doi.org/10.6084/m9.figshare.c.3757043_d1 The current record is supplemented by this dataset	dataset	April 2017
MOESM1 of Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage Φ X174 in Escherichia coli Yung, Mimi; Bourguet, Feliza; Carpenter, Timothy https://doi.org/10.6084/m9.figshare.c.3757043_d1.v1 The current record is supplemented by this dataset	dataset	April 2017