Biodegradation of alkaline lignin by Bacillus ligniniphilus L1

Zhu, Daochen; Zhang, Peipei; Xie, Changxiao; Zhang, Weimin; Sun, Jianzhong; Qian, Wei-Jun; Yang, Bin

doi:10.1186/s13068-017-0735-y

Biodegradation of alkaline lignin by Bacillus ligniniphilus L1

Journal Article · Tue Feb 21 00:00:00 EST 2017 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-017-0735-y· OSTI ID:1357047

Zhu, Daochen ^[1]; Zhang, Peipei ^[2]; Xie, Changxiao ^[2]; Zhang, Weimin ^[3]; Sun, Jianzhong ^[2]; Qian, Wei-Jun ^[4]; Yang, Bin ^[5]

Jiangsu Univ., Zhenjiang, Jiangsu (China). School of Environment and Safety Engineering; Guangdong Inst. of Microbiology, Guangzhou, Guangdong (China). State Key Lab. of Microbial Culture Collection and Application
Jiangsu Univ., Zhenjiang, Jiangsu (China). School of Environment and Safety Engineering
Guangdong Inst. of Microbiology, Guangzhou, Guangdong (China). State Key Lab. of Microbial Culture Collection and Application
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division and Environmental Molecular Sciences Lab.
Washington State Univ., Pullman, WA (United States). Bioproducts, Sciences and Engineering Lab., Dept. of Biological Systems Engineering

Lignin is the most abundant aromatic biopolymer in the biosphere and it comprises up to 30% of plant biomass. Although lignin is the most recalcitrant component of the plant cell wall, still there are microorganisms able to decompose it or degrade it. Fungi are recognized as the most widely used microbes for lignin degradation. However, bacteria have also been known to be able to utilize lignin as a carbon or energy source. Bacillus ligniniphilus L1 was selected in this study due to its capability to utilize alkaline lignin as a single carbon or energy source and its excellent ability to survive in extreme environments. To investigate the aromatic metabolites of strain L1 decomposing alkaline lignin, GC–MS analysis was performed and fifteen single phenol ring aromatic compounds were identified. The dominant absorption peak included phenylacetic acid, 4-hydroxy-benzoicacid, and vanillic acid with the highest proportion of metabolites resulting in 42%. Comparison proteomic analysis was carried out for further study showed that approximately 1447 kinds of proteins were produced, 141 of which were at least twofold up-regulated with alkaline lignin as the single carbon source. The up-regulated proteins contents different categories in the biological functions of protein including lignin degradation, ABC transport system, environmental response factors, protein synthesis, assembly, etc. In conclusion, GC–MS analysis showed that alkaline lignin degradation of strain L1 produced 15 kinds of aromatic compounds. Comparison proteomic data and metabolic analysis showed that to ensure the degradation of lignin and growth of strain L1, multiple aspects of cells metabolism including transporter, environmental response factors, and protein synthesis were enhanced. Based on genome and proteomic analysis, at least four kinds of lignin degradation pathway might be present in strain L1, including a Gentisate pathway, the benzoic acid pathway and the β-ketoadipate pathway. The study provides an important basis for lignin degradation by bacteria.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC05-76RL01830; EE0006112

OSTI ID:: 1357047

Alternate ID(s):: OSTI ID: 1355089

Report Number(s):: PNNL-SA--124233; PII: 735

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Journal Issue: 1 Vol. 10; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Alkaline lignin
Bacillus ligniniphilus L1
GC–MS
Proteomics

Biodegradation of alkaline lignin by Bacillus ligniniphilus L1 Zhu, Daochen; Zhang, Peipei; Xie, Changxiao https://doi.org/10.6084/m9.figshare.c.3699898 The current record is supplemented by this other	other	February 2017
Biodegradation of alkaline lignin by Bacillus ligniniphilus L1 Zhu, Daochen; Zhang, Peipei; Xie, Changxiao https://doi.org/10.6084/m9.figshare.c.3699898.v1 The current record is supplemented by this dataset	dataset	February 2017
MOESM2 of Biodegradation of alkaline lignin by Bacillus ligniniphilus L1 Zhu, Daochen; Zhang, Peipei; Xie, Changxiao https://doi.org/10.6084/m9.figshare.c.3699898_d1 The current record is supplemented by this dataset	dataset	January 2017
MOESM2 of Biodegradation of alkaline lignin by Bacillus ligniniphilus L1 Zhu, Daochen; Zhang, Peipei; Xie, Changxiao https://doi.org/10.6084/m9.figshare.c.3699898_d1.v1 The current record is supplemented by this dataset	dataset	February 2017
MOESM1 of Biodegradation of alkaline lignin by Bacillus ligniniphilus L1 Zhu, Daochen; Zhang, Peipei; Xie, Changxiao https://doi.org/10.6084/m9.figshare.c.3699898_d2 The current record is supplemented by this dataset	dataset	January 2017
MOESM1 of Biodegradation of alkaline lignin by Bacillus ligniniphilus L1 Zhu, Daochen; Zhang, Peipei; Xie, Changxiao https://doi.org/10.6084/m9.figshare.c.3699898_d2.v1 The current record is supplemented by this dataset	dataset	February 2017

Biodegradation of alkaline lignin by Bacillus ligniniphilus L1

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