Light-sheet microscopy by confocal line scanning of dual-Bessel beams
- Washington Univ., St. Louis, MO (United States)
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Here, we have developed a light-sheet microscope that uses confocal scanning of dual-Bessel beams for illumination. A digital micromirror device (DMD) is placed in the intermediate image plane of the objective used to collect fluorescence and is programmed with two lines of pixels in the “on” state such that the DMD functions as a spatial filter to reject the out-of-focus background generated by the side-lobes of the Bessel beams. The optical sectioning and out-of-focus background rejection capabilities of this microscope were demonstrated by imaging of fluorescently stained actin in human A431 cells. The dual-Bessel beam system enables twice as many photons to be detected per imaging scan, which is useful for low light applications (e.g., single-molecule localization) or imaging at high speed with a superior signal to noise. While demonstrated for two Bessel beams, this approach is scalable to a larger number of beams.
- Research Organization:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Organization:
- USDOE Laboratory Directed Research and Development (LDRD) Program
- Grant/Contract Number:
- AC52-06NA25396
- OSTI ID:
- 1356125
- Report Number(s):
- LA-UR-15-24283
- Journal Information:
- Journal of Biomedical Optics, Vol. 21, Issue 10; ISSN 1083-3688
- Publisher:
- SPIECopyright Statement
- Country of Publication:
- United States
- Language:
- English
Web of Science
Technical implementations of light sheet microscopy
|
journal | January 2018 |
Minutes-timescale 3D isotropic imaging of entire organs at subcellular resolution by content-aware compressed-sensing light-sheet microscopy
|
journal | April 2020 |
Light sheet approaches for improved precision in 3D localization-based super-resolution imaging in mammalian cells [Invited]
|
journal | January 2018 |
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