skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Differential CLE peptide perception by plant receptors implicated from structural and functional analyses of TDIF-TDR interactions

Journal Article · · PLoS ONE
 [1];  [1]; ORCiD logo [1];  [2]
  1. North Carolina State Univ., Raleigh, NC (United States)
  2. Univ. of Queensland (Australia)
+ Show Author Affiliations

Tracheary Element Differentiation Inhibitory Factor (TDIF) belongs to the family of post-translationally modified CLE (CLAVATA3/embryo surrounding region (ESR)-related) peptide hormones that control root growth and define the delicate balance between stem cell proliferation and differentiation in SAM (shoot apical meristem) or RAM (root apical meristem). In Arabidopsis, Tracheary Element Differentiation Inhibitory Factor Receptor (TDR) and its ligand TDIF signaling pathway is involved in the regulation of procambial cell proliferation and inhibiting its differentiation into xylem cells. Here we present the crystal structures of the extracellular domains (ECD) of TDR alone and in complex with its ligand TDIF resolved at 2.65 Åand 2.75 Å respectively. These structures provide insights about the ligand perception and specific interactions between the CLE peptides and their cognate receptors. Our in vitro biochemical studies indicate that the interactions between the ligands and the receptors at the C-terminal anchoring site provide conserved binding. While the binding interactions occurring at the N-terminal anchoring site dictate differential binding specificities between different ligands and receptors. Our studies will open different unknown avenues of TDR-TDIF signaling pathways that will enhance our knowledge in this field highlighting the receptor ligand interaction, receptor activation, signaling network, modes of action and will serve as a structure function relationship model between the ligand and the receptor for various similar leucine-rich repeat receptor-like kinases (LRR-RLKs).

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Organization:
USDOE
OSTI ID:
1355045
Journal Information:
PLoS ONE, Vol. 12, Issue 4; ISSN 1932-6203
Publisher:
Public Library of ScienceCopyright Statement
Country of Publication:
United States
Language:
ENGLISH
Citation Metrics:
Cited by: 13 works
Citation information provided by
Web of Science

References (45)

The CLAVATA1 Receptor-like Kinase Requires CLAVATA3 for Its Assembly into a Signaling Complex That Includes KAPP and a Rho-Related Protein journal March 1999
Overview of the CCP 4 suite and current developments journal March 2011
Arabidopsis CLV3 Peptide Directly Binds CLV1 Ectodomain journal January 2008
Structural insight into brassinosteroid perception by BRI1 journal June 2011
Dodeca-CLE Peptides as Suppressors of Plant Stem Cell Differentiation journal August 2006
Coot model-building tools for molecular graphics journal November 2004
Structural insight into the activation of plant receptor kinases journal August 2014
Crystal structure of porcine ribonuclease inhibitor, a protein with leucine-rich repeats journal December 1993
TDIF Peptide Signaling Regulates Vascular Stem Cell Proliferation via the WOX4 Homeobox Gene in Arabidopsis journal August 2010
Plant GSK3 proteins regulate xylem cell differentiation downstream of TDIF–TDR signalling journal March 2014
Maturation processes and structures of small secreted peptides in plants journal July 2014
CLE peptide signaling in plants - the power of moving around journal July 2015
Non-cell-autonomous control of vascular stem cell fate by a CLE peptide/receptor system journal September 2008
Structure-function Aspects of Extracellular Leucine-rich Repeat-containing Cell Surface Receptors in Plants: eLRR Receptor Structure and Function journal September 2013
Peptide Signaling in Plant Development journal May 2011
Dependence of Stem Cell Fate in Arabidopsis on a Feedback Loop Regulated by CLV3 Activity journal July 2000
The PXY-CLE41 receptor ligand pair defines a multifunctional pathway that controls the rate and orientation of vascular cell division journal February 2010
CLE peptide signaling during plant development journal December 2009
Structure–function analysis of the GmRIC1 signal peptide and CLE domain required for nodulation control in soybean journal February 2013
The CLAVATA3/ESR Motif of CLAVATA3 Is Functionally Independent from the Nonconserved Flanking Sequences journal June 2006
Gain-of-Function Phenotypes of Chemically Synthetic CLAVATA3/ESR-Related (CLE) Peptides in Arabidopsis thaliana and Oryza sativa journal December 2007
Macromolecular TLS Refinement in REFMAC at Moderate Resolutions book January 2003
PHENIX : building new software for automated crystallographic structure determination journal October 2002
Plant CLE peptides from two distinct functional classes synergistically induce division of vascular cells journal November 2008
Dual assay for MCLV3 activity reveals structure–activity relationship of CLE peptides journal December 2008
Posttranslationally Modified Small-Peptide Signals in Plants journal April 2014
The CLE family of plant polypeptide signaling molecules journal November 2007
The CLAVATA1Gene Encodes a Putative Receptor Kinase That Controls Shoot and Floral Meristem Size in Arabidopsis journal May 1997
Protein–protein interaction and quaternary structure journal May 2008
Structural Basis for flg22-Induced Activation of the Arabidopsis FLS2-BAK1 Immune Complex journal October 2013
Antagonistic Peptide Technology for Functional Dissection of CLV3/ESR Genes in Arabidopsis journal January 2013
A Polypeptide from Tomato Leaves Induces Wound-Inducible Proteinase Inhibitor Proteins journal August 1991
Crystal structure of the plant receptor-like kinase TDR in complex with the TDIF peptide journal August 2016
A Plant Peptide Encoded by CLV3 Identified by in Situ MALDI-TOF MS Analysis journal August 2006
The Arabidopsis Unannotated Secreted Peptide Database, a Resource for Plant Peptidomics journal September 2006
Structural basis for recognition of an endogenous peptide by the plant receptor kinase PEPR1 journal December 2014
Inference of Macromolecular Assemblies from Crystalline State journal September 2007
Crystal structure of PXY-TDIF complex reveals a conserved recognition mechanism among CLE peptide-receptor pairs journal April 2016
[20] Processing of X-ray diffraction data collected in oscillation mode book January 1997
A glycopeptide regulating stem cell fate in Arabidopsis thaliana journal June 2009
Molecular Mechanism for Plant Steroid Receptor Activation by Somatic Embryogenesis Co-Receptor Kinases journal August 2013
Genome-wide annotation and characterization of CLAVATA/ESR (CLE) peptide hormones of soybean ( Glycine max ) and common bean ( Phaseolus vulgaris ), and their orthologues of Arabidopsis thaliana journal July 2015
Receptor binding and priming of the spike protein of SARS-CoV-2 for membrane fusion journal September 2020
Gain-of-Function Phenotypes of Chemically Synthetic CLAVATA3/ESR-Related (CLE) Peptides in Arabidopsis thaliana and Oryza sativa journal April 2008
Constructing Signal Transduction Pathways in Arabidopsis journal March 1999

Cited By (4)

CLE peptide-mediated signaling in shoot and vascular meristem development journal December 2017
The Extracellular Domain of Pollen Receptor Kinase 3 is structurally similar to the SERK family of co-receptors journal February 2018
Stress‐adaptive gene discovery by exploiting collective decision‐making of decentralized plant response systems journal November 2019
Regulation of plant peptide hormones and growth factors by post‐translational modification journal August 2018

Similar Records

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Crystal structure
Arabidopsis thaliana
Alanine
Sequence alignment
Molecular structure
Sequence motif analysis
Cell differentiation
Histidine