Printable enzyme-embedded materials for methane to methanol conversion

Blanchette, Craig D.; Knipe, Jennifer M.; Stolaroff, Joshuah K.; DeOtte, Joshua R.; Oakdale, James S.; Maiti, Amitesh; Lenhardt, Jeremy M.; Sirajuddin, Sarah; Rosenzweig, Amy C.; Baker, Sarah E.

doi:10.1038/ncomms11900

Printable enzyme-embedded materials for methane to methanol conversion

Journal Article · Wed Jun 15 04:00:00 EDT 2016 · Nature Communications

DOI:https://doi.org/10.1038/ncomms11900· OSTI ID:1349013

Blanchette, Craig D. ^[1]; Knipe, Jennifer M. ^[1]; Stolaroff, Joshuah K. ^[1]; DeOtte, Joshua R. ^[1]; Oakdale, James S. ^[1]; Maiti, Amitesh ^[1]; Lenhardt, Jeremy M. ^[1]; Sirajuddin, Sarah ^[2]; Rosenzweig, Amy C. ^[2]; Baker, Sarah E. ^[1]

Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Northwestern Univ., Evanston, IL (United States)

An industrial process for the selective activation of methane under mild conditions would be highly valuable for controlling emissions to the environment and for utilizing vast new sources of natural gas. The only selective catalysts for methane activation and conversion to methanol under mild conditions are methane monooxygenases (MMOs) found in methanotrophic bacteria; however, these enzymes are not amenable to standard enzyme immobilization approaches. Using particulate methane monooxygenase (pMMO), we create a biocatalytic polymer material that converts methane to methanol. We demonstrate embedding the material within a silicone lattice to create mechanically robust, gas-permeable membranes, and direct printing of micron-scale structures with controlled geometry. Remarkably, the enzymes retain up to 100% activity in the polymer construct. The printed enzyme-embedded polymer motif is highly flexible for future development and should be useful in a wide range of applications, especially those involving gas–liquid reactions.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1349013

Report Number(s):: LLNL--JRNL-678269

Journal Information:: Nature Communications, Journal Name: Nature Communications Vol. 7; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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