An early secretory pathway mediated by GNOM-LIKE 1 and GNOM is essential for basal polarity establishment in Arabidopsis thaliana

Doyle, Siamsa M.; Haeger, Ash; Vain, Thomas; Rigal, Adeline; Viotti, Corrado; Łangowska, Małgorzata; Ma, Qian; Friml, Jiří; Raikhel, Natasha V.; Hicks, Glenn R.; Robert, Stéphanie

doi:10.1073/pnas.1424856112

An early secretory pathway mediated by GNOM-LIKE 1 and GNOM is essential for basal polarity establishment in Arabidopsis thaliana

Journal Article · Mon Feb 02 00:00:00 EST 2015 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1424856112· OSTI ID:1348921

^[1]; Haeger, Ash ^[2]; Vain, Thomas ^[2]; Rigal, Adeline ^[2]; Viotti, Corrado ^[3]; Łangowska, Małgorzata ^[2]; Ma, Qian ^[2]; Friml, Jiří ^[4]; Raikhel, Natasha V. ^[5]; Hicks, Glenn R. ^[5]; Robert, Stéphanie ^[2]

Swedish Univ. of Agricultural Sciences (Sweden); DOE Office of Scientific and Technical Information (OSTI)
Swedish Univ. of Agricultural Sciences (Sweden)
Umea Univ. (Sweden); Univ. of Potsdam (Germany)
Ghent Univ. (Belgium); Institute of Science and Technology Austria (Austria)
Univ. of California, Riverside, CA (United States)

Spatial regulation of the plant hormone indole-3-acetic acid (IAA, or auxin) is essential for plant development. Auxin gradient establishment is mediated by polarly localized auxin transporters, including PIN-FORMED (PIN) proteins. Their localization and abundance at the plasma membrane are tightly regulated by endomembrane machinery, especially the endocytic and recycling pathways mediated by the ADP ribosylation factor guanine nucleotide exchange factor (ARF-GEF) GNOM. We assessed the role of the early secretory pathway in establishing PIN1 polarity in Arabidopsis thaliana by pharmacological and genetic approaches. We identified the compound endosidin 8 (ES8), which selectively interferes with PIN1 basal polarity without altering the polarity of apical proteins. ES8 alters the auxin distribution pattern in the root and induces a strong developmental phenotype, including reduced root length. The ARF-GEF–defective mutants gnom-like 1 (gnl1-1) and gnom (van7) are significantly resistant to ES8. The compound does not affect recycling or vacuolar trafficking of PIN1 but leads to its intracellular accumulation, resulting in loss of PIN1 basal polarity at the plasma membrane. In conclusion, our data confirm a role for GNOM in endoplasmic reticulum (ER)–Golgi trafficking and reveal that a GNL1/GNOM-mediated early secretory pathway selectively regulates PIN1 basal polarity establishment in a manner essential for normal plant development.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of California, Riverside, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: FG02-02ER15295

OSTI ID:: 1348921

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 7 Vol. 112; ISSN 0027-8424

Publisher:: National Academy of Sciences, Washington, DC (United States)Copyright Statement

Country of Publication:: United States

Language:: English

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Localization of Arabidopsis FORKED1 to a RABA-positive compartment suggests a role in secretion Prabhakaran Mariyamma, Neema; Hou, Hongwei; Carland, Francine M. Journal of Experimental Botany, Vol. 68, Issue 13 https://doi.org/10.1093/jxb/erx180	journal	June 2017
Phosphatidylinositol 3-phosphate 5-kinase, FAB1/PIKfyve mediates endosome maturation to establish endosome-cortical microtubule interaction in Arabidopsis Hirano, Tomoko; Munnik, Teun; Sato, Masa H. Plant Physiology https://doi.org/10.1104/pp.15.01368	journal	September 2015
The YABBY Genes of Leaf and Leaf-Like Organ Polarity in Leafless Plant Monotropa hypopitys Shchennikova, Anna V.; Slugina, Marya A.; Beletsky, Alexey V. International Journal of Genomics, Vol. 2018 https://doi.org/10.1155/2018/7203469	journal	January 2018
Genome sequencing and population genomic analyses provide insights into the adaptive landscape of silver birch Salojärvi, Jarkko; Smolander, Olli-Pekka; Nieminen, Kaisa Nature Genetics, Vol. 49, Issue 6 https://doi.org/10.1038/ng.3862	journal	May 2017
Small molecules unravel complex interplay between auxin biology and endomembrane trafficking: Fig. 1. Doyle, Siamsa M.; Vain, Thomas; Robert, Stéphanie Journal of Experimental Botany, Vol. 66, Issue 16 https://doi.org/10.1093/jxb/erv179	journal	April 2015
BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana Kitakura, Saeko; Adamowski, Maciek; Matsuura, Yuki Plant and Cell Physiology, Vol. 58, Issue 10 https://doi.org/10.1093/pcp/pcx118	journal	August 2017
A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN-FORMED protein polarity and relocalization in Arabidopsis Doyle, Siamsa M.; Rigal, Adeline; Grones, Peter bioRxiv https://doi.org/10.1101/422733	posted_content	April 2019
A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN ‐ FORMED protein polarity and relocalisation in Arabidopsis Doyle, Siamsa M.; Rigal, Adeline; Grones, Peter New Phytologist, Vol. 223, Issue 3 https://doi.org/10.1111/nph.15877	journal	May 2019

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