O{sup 6}-methylguanine in DNA inhibits DNA replication and stimulates DNA repair synthesis in vitro
Conference
·
OSTI ID:134872
- Istituto Superiore di Sanita, Rome (Italy)
- Clare Hall Labs., South Mimms (United Kingdom)
O{sup 6}-methylguanine (O{sup 6}-meGua) in DNA does not block replication if purified DNA polymerases are used ina template/primer system, although some slowing of incorporation is apparent. In the SV40 system, we have observed that O{sup 6}-meGua can block replication and at the same time elicit a type of non-semiconservative synthesis that tends to be associated with incompletely repaired, nicked plasmids. It is possible that replication is impaired by the simultaneous occurrence of these {open_quotes}repair{close_quotes} events and that the stimulation of ineffective excision repair at O{sup 6}-meGua in DNA contributes to the cytotoxicity of this methylated base.
- Research Organization:
- New York Academy of Sciences, New York, NY (United States)
- OSTI ID:
- 134872
- Report Number(s):
- CONF-9307221--
- Country of Publication:
- United States
- Language:
- English
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