Excision of uracil from double-stranded DNA by uracil-DNA glycosylase is sequence specific
Conference
·
OSTI ID:134863
- Univ. of Trondheim (Norway)
Removal of uracil in DNA originating from incorporation of dUMP during DNA replication or deamination of cytosine is conducted by uracil-DNA glycosylase (UDG). The best-characterized UDGs are highly conserved single polypeptide-chain enzymes that remove uracil from single-stranded DNA more efficiently than from double-stranded DNA. In an early work by Delort et al., some sequence specificity of UDG on single-stranded DNA was reported. We have applied UDGs from three sources on double-stranded dUMP-containing DNA to establish a putative sequence specificity in uracil-DNA repair. The results reveal a more than 10-fold variation in the rate of repair in different sequences, with identical sequence specificities for UDG from Escherichia coli, calf thymus, and a truncated version of the recombinant human cDNA clone UNG15, which we have recently shown to code for the protein present in both the nucleus and mitochondria of human cells.
- Research Organization:
- New York Academy of Sciences, New York, NY (United States)
- OSTI ID:
- 134863
- Report Number(s):
- CONF-9307221--
- Country of Publication:
- United States
- Language:
- English
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