Base excision repair in E. coli - an overview

Conference · Sat Dec 31 00:00:00 EST 1994

OSTI ID:134847

Kow, Y W ^[1]

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1. Univ. of Vermont, Burlington, VT (United States)

Damages in DNA can arise spontaneously or through the acxtion of envrionmental agents. Oxidants, redox chemicals, as well as ionizing and UV irradiation can produce not only base damages, but also damages to the sufar moiety, generating abasic sites and DNA chain scissions. In E. coli, several pathways exist to facilitate the removal of these lesions. In general, oxidative base damages are repaired by the base excision repair pathway, by which damaged or modified DNA bases are recognized and removed by DNA N-glycosylases. These enzymes hydrolyze the N-glycosylic bond between the modified base and the sugar moiety, leaving behind an abasic (AP) site in DNA. AP sites are then further processed by AP endonucleases, which make incisions either 5{prime} or 3{prime} to the AP sites. The net result of the combined actions of N-glycosylases and AP endonucleases is the formation of a single-base gap in DNA, which can be filled in by DNA polymerase I. DNA repair is completed by a ligase reaction.

• Research Organization: New York Academy of Sciences, New York, NY (United States)
• OSTI ID: 134847
• Report Number(s): CONF-9307221-; CNN: Grant GM 37216; TRN: 95:007741-0013
• Resource Relation: Conference: DNA damage: effects on DNA structure and protein recognition, Burlington, VT (United States), 31 Jul - 4 Aug 1993; Other Information: PBD: 1994; Related Information: Is Part Of DNA damage: Effects on DNA structure and protein recognition; Wallace, S.S.; Van Houten, B.; Kow, Yoke Wah [eds.]; PB: 395 p.
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
DNA
DNA REPAIR
MUTATIONS
GENETIC RADIATION EFFECTS
STRUCTURE-ACTIVITY RELATIONSHIPS
BASES
OXIDATION
ENZYMES
ENZYME ACTIVITY
ENDONUCLEASES
ULTRAVIOLET RADIATION
IONIZING RADIATIONS
HYDROLYSIS
PYRIMIDINES
DNA POLYMERASES
LYASES