The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography

Edlund, Petra; Takala, Heikki; Claesson, Elin; Henry, Léocadie; Dods, Robert; Lehtivuori, Heli; Panman, Matthijs; Pande, Kanupriya; White, Thomas; Nakane, Takanori; Berntsson, Oskar; Gustavsson, Emil; Båth, Petra; Modi, Vaibhav; Roy-Chowdhury, Shatabdi; Zook, James; Berntsen, Peter; Pandey, Suraj; Poudyal, Ishwor; Tenboer, Jason; Kupitz, Christopher; Barty, Anton; Fromme, Petra; Koralek, Jake D.; Tanaka, Tomoyuki; Spence, John; Liang, Mengning; Hunter, Mark S.; Boutet, Sebastien; Nango, Eriko; Moffat, Keith; Groenhof, Gerrit; Ihalainen, Janne; Stojković, Emina A.; Schmidt, Marius; Westenhoff, Sebastian

doi:10.1038/srep35279

Title: The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography

Journal Article · Wed Oct 19 00:00:00 EDT 2016 · Scientific Reports

DOI:https://doi.org/10.1038/srep35279· OSTI ID:1348400

Edlund, Petra ^[1]; Takala, Heikki ^[2]; Claesson, Elin ^[1]; Henry, Léocadie ^[1]; Dods, Robert ^[1]; Lehtivuori, Heli ^[3]; Panman, Matthijs ^[1]; Pande, Kanupriya ^[4]; White, Thomas ^[4]; Nakane, Takanori ^[5]; Berntsson, Oskar ^[1]; Gustavsson, Emil ^[1]; Båth, Petra ^[1]; Modi, Vaibhav ^[3]; Roy-Chowdhury, Shatabdi ^[6]; Zook, James ^[6]; Berntsen, Peter ^[7]; Pandey, Suraj ^[8]; Poudyal, Ishwor ^[8]; Tenboer, Jason ^[8] more »

Univ. of Gothenburg, Gothenburg (Sweden)
Univ. of Gothenburg, Gothenburg (Sweden); Univ. of Jyvaskyla, Jyvaskyla (Finland); Univ. of Helsinki, Helsinki (Finland)
Univ. of Jyvaskyla, Jyvaskyla (Finland)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
The Univ. of Tokyo, Tokyo (Japan)
Arizona State Univ., Tempe, AZ (United States)
LaTrobe Univ., Melbourne, VIC (Australia)
Univ. of Wisconsin, Milwaukee, WI (United States)
SLAC National Accelerator Lab., Menlo Park, CA (United States)
RIKEN SPring-8 Center, Hyogo (Japan)
Univ. of Chicago, Chicago, IL (United States); Argonne National Lab. (ANL), Argonne, IL (United States)
Northern Illinois Univ., Chicago, IL (United States)

Phytochromes are a family of photoreceptors that control light responses of plants, fungi and bacteria. A sequence of structural changes, which is not yet fully understood, leads to activation of an output domain. Time-resolved serial femtosecond crystallography (SFX) can potentially shine light on these conformational changes. Here we report the room temperature crystal structure of the chromophore-binding domains of the Deinococcus radiodurans phytochrome at 2.1 Å resolution. The structure was obtained by serial femtosecond X-ray crystallography from microcrystals at an X-ray free electron laser. We find overall good agreement compared to a crystal structure at 1.35 Å resolution derived from conventional crystallography at cryogenic temperatures, which we also report here. The thioether linkage between chromophore and protein is subject to positional ambiguity at the synchrotron, but is fully resolved with SFX. As a result, the study paves the way for time-resolved structural investigations of the phytochrome photocycle with time-resolved SFX.

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Research Organization:: SLAC National Accelerator Lab., Menlo Park, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1348400

Journal Information:: Scientific Reports, Vol. 6, Issue 1; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 31 works

Citation information provided by
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