Sequence-specific DNA recognition by Cys{sub 2}, His{sub 2} zinc fingers
Conference
·
OSTI ID:134840
- Univ. of Washington, Seattle, WA (United States)
Structural and mutagenesis studies on a variety of zine finger domains have revealed a general scheme by which these motifs perform sequence-specific DNA recognition. As is the4 case for many other types of DNA binding motifs, the side chains responsible for sequence-specific interactions with DNA bases emanate from an {alpha}-helix. Thus, the small motifs can be thought of as protein scaffolds that stabilize and present the helical structure onto which specific DNA binding activities are placed. Unlike the majority of characterized DNA binding motifs, zinc finger proteins do not bind to DNA as dimers and therefore could potentially recognize any specific sequence of DNA, including nonpalidromic sequences. These properties suggest that the Cys{sub 2}, His{sub 2} zinc finger motif may be an excellent starting place for the design of new DNA-binding proteins. Continuing studies in this area are sure to reveal new and intriguing insights to our current understanding of the mechanisms of sequence-specific DNA recognition by proteins.
- Research Organization:
- New York Academy of Sciences, New York, NY (United States)
- OSTI ID:
- 134840
- Report Number(s):
- CONF-9307221--; CNN: Grant P01 GM32681
- Country of Publication:
- United States
- Language:
- English
Similar Records
Signatures of Protein-DNA Recognition in Free DNA Binding Sites
Global analysis of Drosophila Cys2-His2 zinc finger proteins reveals a multitude of novel recognition motifs and binding determinants
GAL4 transcription factor is not a zinc finger but forms a Zn(II) sub 2 Cys sub 6 binuclear cluster
Journal Article
·
Wed Dec 31 23:00:00 EST 2008
· Journal of Molecular Biology
·
OSTI ID:980452
Global analysis of Drosophila Cys2-His2 zinc finger proteins reveals a multitude of novel recognition motifs and binding determinants
Journal Article
·
Wed Mar 06 23:00:00 EST 2013
· Genome Research
·
OSTI ID:1625622
GAL4 transcription factor is not a zinc finger but forms a Zn(II) sub 2 Cys sub 6 binuclear cluster
Journal Article
·
Wed Feb 28 23:00:00 EST 1990
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
·
OSTI ID:6169799