Identifying the metabolic differences of a fast-growth phenotype in Synechococcus UTEX 2973

Mueller, Thomas J.; Ungerer, Justin L.; Pakrasi, Himadri B.; Maranas, Costas D.

doi:10.1038/srep41569

Title: Identifying the metabolic differences of a fast-growth phenotype in Synechococcus UTEX 2973

Journal Article · Tue Jan 31 00:00:00 EST 2017 · Scientific Reports

DOI:https://doi.org/10.1038/srep41569· OSTI ID:1347426

Mueller, Thomas J. ^[1]; Ungerer, Justin L. ^[2]; Pakrasi, Himadri B. ^[2]; Maranas, Costas D. ^[1]

Pennsylvania State Univ., University Park, PA (United States)
Washington Univ., St. Louis, MO (United States)

The photosynthetic capabilities of cyanobacteria make them interesting candidates for industrial bioproduction. One obstacle to large-scale implementation of cyanobacteria is their limited growth rates as compared to industrial mainstays. Synechococcus UTEX 2973, a strain closely related to Synechococcus PCC 7942, was recently identified as having the fastest measured growth rate among cyanobacteria. To facilitate the development of 2973 as a model organism we developed in this study the genome-scale metabolic model iSyu683. Experimental data were used to define CO₂ uptake rates as well as the biomass compositions for each strain. The inclusion of constraints based on experimental measurements of CO₂ uptake resulted in a ratio of the growth rates of Synechococcus 2973 to Synechococcus 7942 of 2.03, which nearly recapitulates the in vivo growth rate ratio of 2.13. This identified the difference in carbon uptake rate as the main factor contributing to the divergent growth rates. Additionally four SNPs were identified as possible contributors to modified kinetic parameters of metabolic enzymes and candidates for further study. As a result, comparisons against more established cyanobacterial strains identified a number of differences between the strains along with a correlation between the number of cytochrome c oxidase operons and heterotrophic or diazotrophic capabilities.

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Research Organization:: Washington Univ., St. Louis, MO (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0012722

OSTI ID:: 1347426

Journal Information:: Scientific Reports, Vol. 7; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 38 works

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Title: Identifying the metabolic differences of a fast-growth phenotype in Synechococcus UTEX 2973

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