Staphylococcal SCCmec elements encode an active MCM-like helicase and thus may be replicative

Mir-Sanchis, Ignacio; Roman, Christina A.; Misiura, Agnieszka; Pigli, Ying Z.; Boyle-Vavra, Susan; Rice, Phoebe A.

doi:10.1038/nsmb.3286

Staphylococcal SCCmec elements encode an active MCM-like helicase and thus may be replicative

Journal Article · Mon Aug 29 04:00:00 EDT 2016 · Nature Structural & Molecular Biology

DOI:https://doi.org/10.1038/nsmb.3286· OSTI ID:1347024

Mir-Sanchis, Ignacio ^[1]; Roman, Christina A. ^[1]; Misiura, Agnieszka ^[2]; Pigli, Ying Z. ^[1]; Boyle-Vavra, Susan ^[3]; Rice, Phoebe A. ^[1]

Univ. of Chicago, IL (United States). Dept. of Biochemistry and Molecular Biology
Univ. of Chicago, IL (United States). Dept. of Biochemistry and Molecular Biology; Abbott Lab., North Chicago, IL (United States)
Univ. of Chicago, IL (United States). Dept. of Pediatrics; Univ. of Chicago, IL (United States). MRSA Research Center

Methicillin-resistant Staphylococcus aureus (MRSA) is a public-health threat worldwide. Although the mobile genomic island responsible for this phenotype, staphylococcal cassette chromosome (SCC), has been thought to be nonreplicative, we predicted DNA-replication-related functions for some of the conserved proteins encoded by SCC. We show that one of these, Cch, is homologous to the self-loading initiator helicases of an unrelated family of genomic islands, that it is an active 3'-to-5' helicase and that the adjacent ORF encodes a single-stranded DNA–binding protein. Our 2.9-Å crystal structure of intact Cch shows that it forms a hexameric ring. Cch, like the archaeal and eukaryotic MCM-family replicative helicases, belongs to the pre–sensor II insert clade of AAA+ ATPases. Additionally, we found that SCC elements are part of a broader family of mobile elements, all of which encode a replication initiator upstream of their recombinases. Replication after excision would enhance the efficiency of horizontal gene transfer.

Research Organization:: Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)

Sponsoring Organization:: USDOE Office of Science (SC); National Institutes of Health (NIH)

OSTI ID:: 1347024

Journal Information:: Nature Structural & Molecular Biology, Journal Name: Nature Structural & Molecular Biology Journal Issue: 10 Vol. 23; ISSN 1545-9993

Publisher:: Nature Publishing Group

Country of Publication:: United States

Language:: ENGLISH

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