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Title: Kinetics of large-scale chromosomal movement during asymmetric cell division in Escherichia coli

Authors:
; ORCiD logo; ; ORCiD logo;
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1346443
Alternate Identifier(s):
OSTI ID: 1344820
Grant/Contract Number:
CNMS2015-231
Resource Type:
Journal Article: Published Article
Journal Name:
PLoS Genetics
Additional Journal Information:
Journal Volume: 13; Journal Issue: 2; Related Information: CHORUS Timestamp: 2017-06-25 07:21:27; Journal ID: ISSN 1553-7404
Publisher:
Public Library of Science (PLoS)
Country of Publication:
United States
Language:
English

Citation Formats

Männik, Jaana, Bailey, Matthew W., O’Neill, Jordan C., Männik, Jaan, and Burkholder, ed., William F. Kinetics of large-scale chromosomal movement during asymmetric cell division in Escherichia coli. United States: N. p., 2017. Web. doi:10.1371/journal.pgen.1006638.
Männik, Jaana, Bailey, Matthew W., O’Neill, Jordan C., Männik, Jaan, & Burkholder, ed., William F. Kinetics of large-scale chromosomal movement during asymmetric cell division in Escherichia coli. United States. doi:10.1371/journal.pgen.1006638.
Männik, Jaana, Bailey, Matthew W., O’Neill, Jordan C., Männik, Jaan, and Burkholder, ed., William F. Fri . "Kinetics of large-scale chromosomal movement during asymmetric cell division in Escherichia coli". United States. doi:10.1371/journal.pgen.1006638.
@article{osti_1346443,
title = {Kinetics of large-scale chromosomal movement during asymmetric cell division in Escherichia coli},
author = {Männik, Jaana and Bailey, Matthew W. and O’Neill, Jordan C. and Männik, Jaan and Burkholder, ed., William F.},
abstractNote = {},
doi = {10.1371/journal.pgen.1006638},
journal = {PLoS Genetics},
number = 2,
volume = 13,
place = {United States},
year = {Fri Feb 24 00:00:00 EST 2017},
month = {Fri Feb 24 00:00:00 EST 2017}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1371/journal.pgen.1006638

Citation Metrics:
Cited by: 3works
Citation information provided by
Web of Science

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  • A new method is presented for determining the growth rate and the probability of cell division (separation) during the cell cycle, using size distributions of cell populations grown under steady-state conditions. The method utilizes the cell life-length distribution, i.e., the probability that a cell will have any specific size during its life history. This method was used to analyze cell length distributions of six cultures of Escherichia coli, for which doubling times varied from 19 to 125 min. The results for each culture are in good agreement with a single model of growth and division kinetics: exponential elongation of cellsmore » during growth phase of the cycle, and normal distributions of length at birth and at division. The average value of the coefficient of variation was 13.5% for all strains and growth rates. These results, based upon 5955 observations, support and extend earlier proposals that growth and division patterns of E. coli are similar at all growth rates and, in addition, identify the general growth pattern of these cells to be exponential.« less
  • The magnitudes of several pools of radioactively labeled precursors for RNA and protein synthesis were determined as a function of cell age during the division cycle of Escherichia coli 15 THU. Uracil, histidine, and methionine pools increased from low initial values for cells at birth to maxima during midcycle and then subsided again. These pools were small or nonexistent at the beginning and the end of the cycle, and their average values during the cycle were less that 4% of the total cellular radioactivity. The results are consistent with a linear pattern of growth for cells during the division cyclemore » and provide strong evidence against exponential or bilinear growth of E. coli cells.« less
  • Increase in the mean cell mass of undivided cells was determined during the division cycle of Escherichia coli B/rA. Cell buoyant densities during the division cycle were determined after cells from an exponentially growing culture were separated by size. The buoyant densities of these cells were essentially independent of cell age, with a mean value of 1.094 g ml/sup -1/. Mean cell volume and buoyant density were also determined during synchronous growth in two different media, which provided doubling times of 40 and 25 min. Cell volume and mass increased linearly at both growth rates, as buoyant density did notmore » vary significantly. The results are consistent with only one of the three major models of cell growth, linear growth, which specifies that the rate of increase in cell mass is constant throughout the division cycle.« less