High-throughput simulations reveal membrane-mediated effects of alcohols on MscL gating

Melo, Manuel N.; Arnarez, Clement; Sikkema, Hendrik; Kumar, Neeraj; Walko, Martin; Berendsen, Herman J. C.; Kocer, Armagan; Marrink, Siewert J.; Ingolfsson, Helgi I.

doi:10.1021/jacs.6b11091

High-throughput simulations reveal membrane-mediated effects of alcohols on MscL gating

Journal Article · Thu Jan 26 00:00:00 EST 2017 · Journal of the American Chemical Society

DOI:https://doi.org/10.1021/jacs.6b11091· OSTI ID:1346131

Melo, Manuel N. ^[1]; Arnarez, Clement ^[1]; Sikkema, Hendrik ^[1]; Kumar, Neeraj ^[1]; Walko, Martin ^[1]; Berendsen, Herman J. C. ^[1]; Kocer, Armagan ^[1]; Marrink, Siewert J. ^[1]; ^[2]

Univ. of Groningen, Groningen (The Netherlands)
Univ. of Groningen, Groningen (The Netherlands); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

The mechanosensitive channels of large conductance (MscL) are bacterial membrane proteins that serve as last resort emergency release valves in case of severe osmotic downshock. Sensing bilayer tension, MscL channels are sensitive to changes in the bilayer environment and are, therefore, an ideal test case for exploring membrane protein coupling. Here, we use high-throughput coarse-grained molecular dynamics simulations to characterize MscL gating kinetics in different bilayer environments under the influence of alcohols. We performed over five hundred simulations to obtain sufficient statistics to reveal the subtle effects of changes in the membrane environment on MscL gating. MscL opening times were found to increase with the addition of the straight-chain alcohols ethanol, octanol, and to some extent dodecanol but not with hexadecanol. Increasing concentration of octanol increased the impeding effect, but only up to 10–20 mol %. Our in silico predictions were experimentally confirmed using reconstituted MscL in a liposomal fluorescent efflux assay. Our combined data reveal that the effect of alcohols on MscL gating arises not through specific binding sites but through a combination of the alcohol-induced changes to a number of bilayer properties and their alteration of the MscL–bilayer interface. Finally, our work provides a key example of how extensive molecular simulations can be used to predict the functional modification of membrane proteins by subtle changes in their bilayer environment.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1346131

Report Number(s):: LLNL-JRNL--715277

Journal Information:: Journal of the American Chemical Society, Journal Name: Journal of the American Chemical Society Journal Issue: 7 Vol. 139; ISSN 0002-7863

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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