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Title: Enhancing photo-catalytic production of organic acids in the cyanobacterium S ynechocystis sp. PCC 6803 Δ glg C , a strain incapable of glycogen storage

Abstract

We describe how a key objective in microbial biofuels strain development is to maximize carbon flux to target products while minimizing cell biomass accumulation, such that ideally the algae and bacteria would operate in a photo-catalytic state. A brief period of such a physiological state has recently been demonstrated in the cyanobacterium Synechocystis sp. PCC 6803 ΔglgC strain incapable of glycogen storage. When deprived of nitrogen, the ΔglgC excretes the organic acids alpha-ketoglutarate and pyruvate for a number of days without increasing cell biomass. This study examines the relationship between the growth state and the photo-catalytic state, and characterizes the metabolic adaptability of the photo-catalytic state to increasing light intensity. It is found that the culture can transition naturally from the growth state into the photo-catalytic state when provided with limited nitrogen supply during the growth phase. Photosynthetic capacity and pigments are lost over time in the photo-catalytic state. Reversal to growth state is observed with re-addition of nitrogen nutrient, accompanied by restoration of photosynthetic capacity and pigment levels in the cells. While the overall productivity increased under high light conditions, the ratio of alpha-ketoglutarate/pyruvate is altered, suggesting that carbon partition between the two products is adaptable to environmental conditions.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States). Biosciences Center
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Fuel Cell Technologies Office (EE-3F)
OSTI Identifier:
1345698
Alternate Identifier(s):
OSTI ID: 1220612; OSTI ID: 1345699
Report Number(s):
NREL/JA-2700-62489
Journal ID: ISSN 1751-7915
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Journal Article: Published Article
Journal Name:
Microbial Biotechnology (Online)
Additional Journal Information:
Journal Name: Microbial Biotechnology (Online); Journal Volume: 8; Journal Issue: 2; Journal ID: ISSN 1751-7915
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Synechocystis sp.; photo-catalysis, organic acids

Citation Formats

Carrieri, Damian, Broadbent, Charlie, Carruth, David, Paddock, Troy, Ungerer, Justin, Maness, Pin-Ching, Ghirardi, Maria, and Yu, Jianping. Enhancing photo-catalytic production of organic acids in the cyanobacterium S ynechocystis sp. PCC 6803 Δ glg C , a strain incapable of glycogen storage. United States: N. p., 2015. Web. doi:10.1111/1751-7915.12243.
Carrieri, Damian, Broadbent, Charlie, Carruth, David, Paddock, Troy, Ungerer, Justin, Maness, Pin-Ching, Ghirardi, Maria, & Yu, Jianping. Enhancing photo-catalytic production of organic acids in the cyanobacterium S ynechocystis sp. PCC 6803 Δ glg C , a strain incapable of glycogen storage. United States. doi:10.1111/1751-7915.12243.
Carrieri, Damian, Broadbent, Charlie, Carruth, David, Paddock, Troy, Ungerer, Justin, Maness, Pin-Ching, Ghirardi, Maria, and Yu, Jianping. Fri . "Enhancing photo-catalytic production of organic acids in the cyanobacterium S ynechocystis sp. PCC 6803 Δ glg C , a strain incapable of glycogen storage". United States. doi:10.1111/1751-7915.12243.
@article{osti_1345698,
title = {Enhancing photo-catalytic production of organic acids in the cyanobacterium S ynechocystis sp. PCC 6803 Δ glg C , a strain incapable of glycogen storage},
author = {Carrieri, Damian and Broadbent, Charlie and Carruth, David and Paddock, Troy and Ungerer, Justin and Maness, Pin-Ching and Ghirardi, Maria and Yu, Jianping},
abstractNote = {We describe how a key objective in microbial biofuels strain development is to maximize carbon flux to target products while minimizing cell biomass accumulation, such that ideally the algae and bacteria would operate in a photo-catalytic state. A brief period of such a physiological state has recently been demonstrated in the cyanobacterium Synechocystis sp. PCC 6803 ΔglgC strain incapable of glycogen storage. When deprived of nitrogen, the ΔglgC excretes the organic acids alpha-ketoglutarate and pyruvate for a number of days without increasing cell biomass. This study examines the relationship between the growth state and the photo-catalytic state, and characterizes the metabolic adaptability of the photo-catalytic state to increasing light intensity. It is found that the culture can transition naturally from the growth state into the photo-catalytic state when provided with limited nitrogen supply during the growth phase. Photosynthetic capacity and pigments are lost over time in the photo-catalytic state. Reversal to growth state is observed with re-addition of nitrogen nutrient, accompanied by restoration of photosynthetic capacity and pigment levels in the cells. While the overall productivity increased under high light conditions, the ratio of alpha-ketoglutarate/pyruvate is altered, suggesting that carbon partition between the two products is adaptable to environmental conditions.},
doi = {10.1111/1751-7915.12243},
journal = {Microbial Biotechnology (Online)},
number = 2,
volume = 8,
place = {United States},
year = {Fri Jan 23 00:00:00 EST 2015},
month = {Fri Jan 23 00:00:00 EST 2015}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1111/1751-7915.12243

Citation Metrics:
Cited by: 4 works
Citation information provided by
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