Cerenkov luminescence endoscopy: Improved molecular sensitivity with β--emitting radiotracers

Carpenter, Colin M.; Ma, Xiaowei; Liu, Hongguang; Sun, Conroy; Pratx, Guillem; Wang, Jing; Gambhir, Sanjiv S.; Xing, Lei; Cheng, Zhen

doi:10.2967/jnumed.114.139105

Title: Cerenkov luminescence endoscopy: Improved molecular sensitivity with β^--emitting radiotracers

Journal Article · Thu Oct 09 00:00:00 EDT 2014 · Journal of Nuclear Medicine

DOI:https://doi.org/10.2967/jnumed.114.139105· OSTI ID:1345590

Carpenter, Colin M. ^[1]; Ma, Xiaowei ^[2]; Liu, Hongguang ^[1]; Sun, Conroy ^[1]; Pratx, Guillem ^[1]; Wang, Jing ^[3]; Gambhir, Sanjiv S. ^[1]; Xing, Lei ^[4]; Cheng, Zhen ^[1]

Stanford Univ., Stanford, CA (United States)
Stanford Univ., Stanford, CA (United States); Fourth Military Medical Univ., Shaanxi (China)
Fourth Military Medical Univ., Shaanxi (China)
Stanford Univ., Stanford, CA (United States); Stanford Univ. School of Medicine, Stanford, CA (United States)

Cerenkov luminescence endoscopy (CLE) is an optical technique that captures the Cerenkov photons emitted from highly energetic moving charged particles (β⁺ or β^$$-$$) and can be used to monitor the distribution of many clinically available radioactive probes. A main limitation of CLE is its limited sensitivity to small concentrations of radiotracer, especially when used with a light guide. We investigated the improvement in the sensitivity of CLE brought about by using a β^$$-$$ radiotracer that improved Cerenkov signal due to both higher β-particle energy and lower γ noise in the imaging optics because of the lack of positron annihilation. Here, the signal-to-noise ratio (SNR) of ⁹⁰Y was compared with that of ¹⁸F in both phantoms and small-animal tumor models. Sensitivity and noise characteristics were demonstrated using vials of activity both at the surface and beneath 1 cm of tissue. Rodent U87MG glioma xenograft models were imaged with radiotracers bound to arginine-glycine-aspartate (RGD) peptides to determine the SNR. As a result, γ noise from ¹⁸F was demonstrated by both an observed blurring across the field of view and a more pronounced fall-off with distance. A decreased γ background and increased energy of the β particles resulted in a 207-fold improvement in the sensitivity of ⁹⁰Y compared with ¹⁸F in phantoms. ⁹⁰Y-bound RGD peptide produced a higher tumor-to-background SNR than ¹⁸F in a mouse model. In conclusion, the use of ⁹⁰Y for Cerenkov endoscopic imaging enabled superior results compared with an ¹⁸F radiotracer.

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Research Organization:: Stanford Univ., CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Natural Science Foundation of China (NSFC)

Grant/Contract Number:: SC0008397; W81XWH-11-1-0087; W81XWH-11-1-0070; W81XWH-10-1-0506

OSTI ID:: 1345590

Journal Information:: Journal of Nuclear Medicine, Vol. 55, Issue 11; ISSN 0161-5505

Publisher:: Society of Nuclear Medicine and Molecular ImagingCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 36 works

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