Detection of bcr/abl fusion and duplication of abl in a Ph-negative CML patient by the dual-color FISH method
- Univ. of Texas, Houston, TX (United States)
We report here the use of the fluorescence in situ hybridization (FISH) method to detect duplication of the abl gene and fusion of bcr/abl in a Ph-negative CML patient. The patient was in clinical remission when studied. Conventional cytogenetic analysis revealed an apparent normal diploid karyotype. Using a metaphase-FISH method previously described for detecting residual leukemic cells in CML patients in clinical remission, we failed to detect any gross abnormality involving chromsomes 9 and 22. However, using the dual-color bcr and abl probes (Oncor), we found that in 55% of the interphases, fusion of these two genes was detectable. More interestingly, in those cells in which fusion of the bcr and abl genes occurred, we observed an extra abl signal. Further examination of bcr and abl hybridization signals on metaphases revealed that the bcr/abl fusion occurred on a chromosome that resembled chromosome 22. The two abl signals were on two chromosomes that resembled chromosome 9s and one bcr signal could be found on another chromosome 22. Thus the bcr and abl dual-color probes allow us to detect a rare form of gene fusion and duplication that is not detectable by karyotyping or chromosome painting.
- OSTI ID:
- 134559
- Report Number(s):
- CONF-941009--
- Journal Information:
- American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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